ORIGINAL ARTICLE
Water soluble antifungal metabolites of pea (Pisum sativum) leaves determine infection by Erysiphe pisi
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Department of Mycology and Plant Pathology, Institute of Agricultural Sciences Banaras Hindu University, Varanasi-221005, India
Corresponding author
Amar Bahadur
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences Banaras Hindu University, Varanasi-221005, India
Journal of Plant Protection Research 2009;49(2):216-220
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ABSTRACT
Antifungal activity of water soluble exudates of pea leaves was assayed. Exudates of different nodal leaves were collected by dipping them in sterilized distilled water for different length of time andconidial germination of four non-pathogenic to pea fungi (Alternaria solani, Curvularia lunata, Helminthosporium penniseti and H. echinocloa) and one pea pathogenic to pea fungus (Erysiphe pisi) was observed in the collected exudates. The leaf exudates inhibited differently conidial germination and A. solani and C.lunata were found highly susceptible. Conidial germination on exudate-depleted pea leaves showed better germination of E. pisi than on non-depleted leaves. Bipolar germination was the highest on II and III nodal leaves dipped in distilled water for 18 h while after 24 h it was the highest on I nodal leaves following dipping in distilled water. Similarly, conidial germination of E. pisi was observed on different nodal leaves of pea of different age. It was very clear that young leaves did not support germination at all and on the same nodal leaves conidia germinated after the leaves became older. No germination was observed on less than 20-day-old leaves. Moreover, 30-day-old leaves were found to be highly susceptible as bipolar germination of E. pisi conidia was maximum on such leaves and did not increase with aging
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (12)
1.
Carver T.L.W., Adaigbe M.E. 1990. Effect of oat genotype, leaf age and position and incubation humidity on germination and germling development by Erysiphe graminis f. sp. avenae. Mycol. Res. 94: 18–26.
2.
Flentje N.T. 1959. Problems and progress. p. 76–87. In: “Plant Pathology” (C.S. Holton, ed.). University of Wisconsis Press, Madison.
3.
Hilu H.M. 1965. Host-pathogen relationships of Puccinia sorghi in nearly isogenic resistant and susceptible seedling corn. Phytopathology 55: 563–569.
4.
Kozel P.C., Tukey H.B.Jr. 1968. Loss or gibberellins by leaching from stems and foliage of Chrysanthemum morifolium‘Princess‘ Anne’. Am. J. Bot. 55: 1184–1189.
5.
Leath K.T., Rowell J.B. 1966. Histological study of the resistance of Zea mays to Puccinia graminis. Phytopathology 56: 1305–1309.
6.
Mitchell C.A. 1968. Detection of carbohydrates leached from above ground plant parts. M.S. Thesis, Cornell Univ., Ithaca, N.Y.
7.
Mohanty C.R., Gangopadhyay S. 1981. Germination of Pyricularia oryzae spores in the leaf exudates of six rice varieties. Indian Phytopathol. 34: 296–299.
8.
Saxena B.N. 1978. Rice leaf exudates in relation to blast disease. Indian Phytopathol. 31: 196–198.
9.
Sadasivan T.S.S., Suryanarayan, Ramakrishna L. 1965. Effect of night temperature on pathogenicity in the rice blat disease. Proc. Symp. IRRI, 163 pp.
10.
Tukey H.B.Jr. 1966. Leaching metabolites from above-ground plant parts and its implication. Bull. Torrey Bot. Club. 93: 385–401.
11.
Tukey H.B.Jr. 1969. Implications of allelopathy in agricultural plant science. Bot. Rev. 35: 1–16.
12.
Yamada Y., Bukovac M.J., Wittwer S.H. 1964. Penetration of ions through isolated cuticles, Plant Physiol. 39: 28–32.