ORIGINAL ARTICLE
Virulence of Alternaria alternata infecting noni associated with production of cell wall degrading enzymes
1
Department of Plant Pathology, Centre for Plant Protection Studies
Tamil Nadu Agricultural University, Coimbatore – 641003, India
Corresponding author
Manjunath Hubballi
Department of Plant Pathology, Centre for Plant Protection Studies Tamil Nadu Agricultural University, Coimbatore – 641003, India
Department of Plant Pathology, Centre for Plant Protection Studies Tamil Nadu Agricultural University, Coimbatore – 641003, India
Journal of Plant Protection Research 2011;51(1):87-92
KEYWORDS
TOPICS
ABSTRACT
Different methods of inoculations were tried for proving Koch postulates. Among them, the pin prick plus spore suspension
spray was found be the best method used in a glasshouse. Out of the fifteen isolates of
Alternaria alternata
, isolate AA1 was highly
virulent and AA6 was avirulent. The virulent isolate of
A. alternata
produced more cellulolytic (C1 and Cx) and pectinolytic (macerat-
ing enzymes, pectin methyl esterase and endo-polygalacturonase) enzymes
in vitro
than the avirulent one. The activity of cellulolytic
enzymes increased with the increase in the age of the culture. Whereas, the pectinolytic enzymes were highly active in 10-day-old
culture and the activities decreased with the increase in the age of the culture. Then again, the activity of enzymes produced by the
avirulent isolate of pathogens did not decrease and these enzyme activities increased with the increase in the age of the culture
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (25)
1.
Anand T. 2002. Studies on fruit rot of chilli (Capsicum annuum L.) caused by Colletotrichum capsici (Syd.) Butler and Bisby and Alternaria alternata(Fr) Keissler. M.Sc. (Ag.) thesis, Tamil Nadu Agric. Univ. Coimbatore, India, 157 pp.
2.
Anand T., Bhaskaran R., Raguchander T., Karthikeyan G., Rajesh M., Senthilraja G. 2008. Production of cell wall degrading enzymes and toxins by Colletotrichum capsici and Alternaria Alternata causing fruit rot of chillies. J. Plant Protection Res. 48 (4): 437–451.
3.
Awasthi R.P., Kolte S.J. 1989. Variability in Alternaria brassicae affecting rape seed and mustard. Indian Phytopathol. 42, p. 863.
4.
Bateman D.F. 1964. Cellulase and the Rhizoctoniadisease of bean. Phytopathology 54 (11):1372–1377.
5.
Bateman D.F., Miller R. 1966. Pectic enzymes in tissue degradation. Annu. Rev. Phytopathol. 4: 119–146.
6.
Bhaskaran R., Kandaswamy T.K. 1978. Production of a toxic metabolite by Alternaria helianthi in vitro and in vivo. Madras Agric. J. 65: 801–804.
7.
Chenglin Y.,William S.C., Carl E.S. 1996. Purification and characterization of polygalacturanase produced by Penicillium expansum in apple fruit. Phytopatholgy 86 (11): 1160–1166.
8.
Gomez K.A., Gomez A.A. 1984. Statistical Procedure for Agricultural Research. Wiley, New York, 680 pp.
9.
Goodman R.N., Kiraly Z., Zaitlin M. 1967. The Biochemistry and Physiology of Infectious Plant Disease. Co., Inc. Princeton, New Jersey, 354 pp.
10.
Gupta T. 1970. In vitro and in vivo production of pectolytic and cellulolytic enzymes by four isolates of Colletotrichum.p. 197–207. In: “Physiology of Parasitism” (G.P. Agarwal, K.S. Bilgrami, eds.). Today and Tomorrow’s, New Delhi, 284 pp.
11.
Hancock J.G., Miller R.L., Lorbeer J.W. 1964. Pectinolytic and cellulolytic enzymes produced by Botrytis alli,Botrytis cinerea and Botrytis squamosa in vitro and in vivo. Phytopathology 54 (8): 928–931.
12.
Jha D.K., Gupta D.P. 1988. Production of pectinolytic enzymes by Alternaria triticina. Indian Phytopathol. 41, p. 652.
13.
Lakshmanan P., Jeyarajan R., Vidhyasekaran P. 1990. A boll rot of cotton caused by Corynespora cassiicola in Tamil Nadu, India. Phytoparasitica 18 (2): 171–173.
14.
Mahadevan A. 1965. Production of protopectinase by Fusarium vasinfectum.Curr. Sci.19, p. 566.
15.
Marimuthu T., Bhaskaran R., Shanmugam N., Purushothaman D. 1974. In vitro production of cell wall splitting enzymes by Alternaria sesami. Labd. J. Sci. Tech. 12B: 26–28.
16.
Mc Kinney H.H. 1923. A new system of grading plant diseases. J. Agric. Res. 26 (2): 195–218.Muthulakshmi P. 1990. Studies on fruit rot of chilli (Capsicum annuum L.) caused by Alternaria tenis Nees.M. Sc. (Ag.) thesis, Tamil Nadu Agric. Univ. Madurai, India, 139 pp.
17.
Nelson S.C. 2006. Species profiles for Pacific island agroforestry, Morinda citrifolia (noni) http://www.traditionaltree.org., p. 18.
18.
Norkrans B. 1950. Influence of cellulolytic enzymes from hyphomycetes on cellulose preparations of different crystallinity. Physiol. Plantarum 3 (3): 75–85.
19.
Peter P.I. 2009. Energetic vibrations of noni. Noni Search 2009 – Fourth National Symposium. World Noni Research Foundation, Chennai, India, 25–26 October 2009, p. 18.
20.
Rangaswami G. 1958. An agar blocks technique for isolating soil micro organisms with special reference to Pythiaceous fungi. Sci. Cult. 24, p. 85.
21.
Riker A.J., Riker R.S. 1936. Introduction to Research on Plant Diseases. John Swift Co., St. Louis, Chicago, 117 pp.
22.
Singh R.S. 2002. Introduction to Principles of Plant Pathology. 4th ed. Oxford IBH Publishing Co. Pvt. Ltd, New Delhi, 398 pp.
23.
TNAU (Tamil Nadu Agricultural University). 1980. Score Chart for Crop Diseases. Coimbatore, India, 56 pp.
24.
Wood R.K.S., Balio A., Graniti A. 1972. Phytotoxins in Plant Disease. Academic Press, New York, 530 pp.
25.
Yang Z., Rogers L.M., Song Y., Guo W., Kolattukudy P.E. 2005. Homoserine and asparagines are host signals that trigger in planta expression of a pathogenesis gene in Nectria haematococca. Proc. Natl. Acad. Sci. USA 102: 4197–4202.
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