ORIGINAL ARTICLE
Vegetative compatibility among Fusarium oxysporum isolates from bitter gourd and bottle gourd in the Philippines
1
Crop Protection Cluster, College of Agriculture, University of the Philippines Los Baños,
College, Laguna 4031 Philippines
2
East West Seed Company, San Ildefonso, Bulacan 3008 Philippines
Corresponding author
Christian Joseph R. Cumagun
Crop Protection Cluster, College of Agriculture, University of the Philippines Los Baños, College, Laguna 4031 Philippines
Crop Protection Cluster, College of Agriculture, University of the Philippines Los Baños, College, Laguna 4031 Philippines
Journal of Plant Protection Research 2008;48(3):283-293
KEYWORDS
Fusarium oxysporum f. sp. momordicaeFusarium oxysporum f. sp. lagenariaebitter gourdbottle gourdVegetative compatibility groups
TOPICS
ABSTRACT
Vegetative compatibility groups (VCGs) were studied in 57 Fusarium oxysporum isolates from Momordica charantia L. (bitter gourd) and Lagenaria siceraria (Mol.) Standley (bottle gourd) using nitrate-non-utilizing (nit) mutants. Out of these, 24 isolates that sectored frequently in chlorate medium were genetically unstable and not further used in the experiment. Only 32 isolates were used, among them 21 from F. oxysporum f. sp. momordicae and 11 from F. oxysporum f. sp. lagenariae. Sixty one nit mutants were generated from 21 isolates F. oxysporum f. sp. momordicae with their respective frequencies: nit1 (31), nit3 (11), nitM (19). Twenty five nit mutants were generated from 11 isolates of F. oxysporum f. sp. lagenariae with their respective frequencies: nit1 (13), nit3 (5), nitM (7). F. oxysporum f. sp. momordicae populations have higher frequency of reversion to wild type (39.4%) than F. oxysporum f. sp. lagenariae (27.3%). Non-reverted mutants were used in complementation pests. Four VCGs of F. oxysporum f. sp. momordicae were identified with the majority belonging to a single VCG. Five VCGs of F. oxysporum f. sp. lagenariae were identified. Low VCG diversity ratio (VCGdiv = 0.19) was
observed for F. oxysporum f. sp. momordicae whereas a higher value (VCGdiv = 0.45) was obtained for F. oxysporum f. sp. lagenariae. F. oxysporum f. sp. momordicae and F. oxysporum f. sp. lagenariae isolates were not vegetatively compatible.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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