ORIGINAL ARTICLE
Utilization of carbohydrates by Beauveria bassiana isolates obtained from forest pests
1
Plant Protection Institute, 35 Panajot Volov Str., 2230 Kostinbrod, Bulgaria
2
Institute of Biodiversity and Ecosystem Research, 1000 Sofia, Bulgaria
3
University of Forestry, 1756 Sofia, Bulgaria
Corresponding author
Slavimira Atanasova Draganova
Plant Protection Institute, 35 Panajot Volov Str., 2230 Kostinbrod, Bulgaria
Plant Protection Institute, 35 Panajot Volov Str., 2230 Kostinbrod, Bulgaria
Journal of Plant Protection Research 2011;51(4):349-354
KEYWORDS
TOPICS
ABSTRACT
Carbohydrate utilization profiles of ten isolates of the entomopathogenic fungus
Beauveria bassiana
(Bals.-Criv.) Vuillemin
were studied and compared to assist in determining their phenotypic characterization. Isolates were obtained in pure cultures from
dead individuals of several forest pests collected from different regions in Bulgaria. Studies on utilization profiles were based on the
acidification of twenty carbohydrates.
The results indicate that sucrose, maltose and trehalose were assimilated at a high degree compared to esculin, arabinose and dulcitol. According to the results of a cluster analysis of the carbohydrate utilization profiles, the
B. bassiana
isolates were divided into two
larger groupings. All isolates in the first larger cluster were obtained from the coleopteran insects –
Stenomax aeneus
,
Ips typographus
,
I. sexdentatus
and
Dryocoetes autographus
. Isolates from the other cluster were obtained from the lepidopteran larvae of
Thaumetopoea
pityocampa
and
Lymantria dispar,
and from an adult of the coleopteran species
Hylurgops palliatus
. We determined that each
B. bassiana
isolate exhibited a different and specific carbohydrate utilization profile but differences at the p-level < 0.05 were significant among
some of them. The most distinguishable was the isolate 560Bb obtained from
T. pityocampa
. Differences between the isolate 560Bb and
the other nine
B. bassiana
isolates were highly significant at the p-level < 0.005. Isolate 433Bb obtained from a dead adult of
I. typogra-
phus
was significantly different from five of the studied isolates at the p-level < 0.05.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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