ORIGINAL ARTICLE
Use of cultural filtrates of certain microbial isolates for powdery mildew control in squash
1
Agricultural Botany Department Faculty of Agriculture Kafr El-Shiekh Univ., Kafr El-Shiekh 33516 Egypt
2
Pesticides Department Faculty of Agriculture Kafr El-Shiekh Univ., Kafr El-Shiekh 33516, Egypt
Corresponding author
Gaber Abd Elwenees Elkot
Agricultural Botany Department Faculty of Agriculture Kafr El-Shiekh Univ., Kafr El-Shiekh 33516 Egypt
Agricultural Botany Department Faculty of Agriculture Kafr El-Shiekh Univ., Kafr El-Shiekh 33516 Egypt
Aly Soliman Hamed Derbalah
Pesticides Department Faculty of Agriculture Kafr El-Shiekh Univ., Kafr El-Shiekh 33516, Egypt
Pesticides Department Faculty of Agriculture Kafr El-Shiekh Univ., Kafr El-Shiekh 33516, Egypt
Journal of Plant Protection Research 2011;51(3):252-260
KEYWORDS
TOPICS
ABSTRACT
Powdery mildew induces significant losses in yield and quality of squash. Therefore, culture filtrates of certain microbial
isolates, (
Epicoccum nigrum, Epicoccum minitans, Epicoccum
sp
., Trichoderma harzianum, Trichoderma viride
and
Bacillus pumilus
) were
used alone, and in combination with the fungicide penconazole to control powdery mildew in squash, under field conditions. Moreover, GC-MS analysis was carried out to identify the chemical components of the most effective culture filtrates against powdery
mildew pathogen. The results showed that culture filtrates of different microbial isolates (except for
Trichoderma harzianum
) were more
effective against powdery mildew in squash than the tested fungicide alone at the recommended levels,
in both tested seasons
.
The
results also showed that mixing different culture filtrates with penconazole improved efficiency against powdery mildew compared
to using the fungicide alone, in both tested seasons. The efficacy of the culture filtrates of the tested microbial isolates against powdery
mildew were due to the presence of a mixture of known antifungal compounds. The results suggest the possible use of the culture
filtrates of the tested microbial isolates as alternative to fungicides, in powdery mildew control. Also, this study suggests the possible
mixing of the culture filtrate of the tested biocontrol agents with fungicides to minimize the applied amount of fungicides.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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