ORIGINAL ARTICLE
Tubercle disease of sugar beets, the lost pathogen, and the thin border between pathogenicity and stimulatory effect
1
Institute of Environmental Engineering and Biotechnology, Opole University, Opole, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-05-31
Acceptance date: 2023-07-17
Online publication date: 2023-08-17
Corresponding author
Ewa B. Moliszewska
Institute of Environmental Engineering and Biotechnology, Opole University, Opole, Poland
Institute of Environmental Engineering and Biotechnology, Opole University, Opole, Poland
Journal of Plant Protection Research 2023;63(3):366-374
HIGHLIGHTS
- Two bacterial strains deposited in the NCPPB at the beginning of the XX century as Xanthomonas beticola (tubercle disease) are not pathogenic to sugar beets.
- They presented stimulatory properties to sugar beets and should be treated as beneficial microorganisms.
- They showed some properties to protect sugar beets against Rhizoctonia solani.
- Their taxonomic status is unclear, however, they belong to the Bacillus genus.
KEYWORDS
TOPICS
ABSTRACT
Tubercle disease or a bacterial pocket disease of sugar beets are names used to describe one
of the gall-malformed diseases of sugar beet roots. Xanthomonas beticola is the historical
name of the pathogen supposedly causing bacterial pocket disease. There were no isolates
deposited in any collection corresponding to the originally isolated bacteria, except two
strains from the NCPPB (National Collection of Plant Pathogenic Bacteria, UK). However,
both isolates were identified as related to Bacillus pumilus, which raised doubts about their
pathogenicity. In our laboratory, greenhouse, and preliminary field experiments, we demonstrated
that such strains are not pathogenic to sugar beets. Furthermore, both strains
promoted their growth, improved their yield quality, and partly protected them against
Rhizoctonia solani in a field experiment.
ACKNOWLEDGEMENTS
Authors wish to acknowledge Ms. Julia Dickenson, the
Polish-American Fulbright Commission grantee, for
her language support.
RESPONSIBLE EDITOR
Natasza Borodynko-Filas
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (34)
2.
Barash I., Manulis-Sasson S. 2007. Virulence mechanisms and host specificity of gall-forming Pantoea agglomerans. Trends in Microbiology 15 (12): 538–545. DOI: 10.1016/j.tim.2007.10.009.
3.
Barash I., Manulis-Sasson, S. 2009. Recent Evolution of Bacterial Pathogens: The Gall-Forming Pantoea agglomerans Case. Annual Review of Phytopathology 47: 133–152. DOI: https://doi.org/10.1146/annure....
4.
Benada J., Špaček J., Šedivy J. 1984. Atlas chorób i szkodników buraka. [Atlas of Beet Diseases and Pests]. PWRiL Warsaw, PL, 264 pp. (in Polish).
5.
Brown N.A. 1928. Bacterial pocket disease of the sugar beet. Journal of Agricultural Research 37 (3): 155–168.
6.
Burr T.J., Katz B.H., Abawi G.S., Crosier D.C. 1991. Comparison of tumorigenic strains of Erwinia herbicola isolated from table beet with E. h. gypsophilae. Plant Disease 75 (8): 855–858. DOI: 10.1094/PD-75-0855.
7.
Draycott A.P. 2006. Sugar beet. Blackwell Publishing Ltd., Oxford, UK, 474 pp. DOI: 10.1017/S0014479706334539.
8.
Enya J., Koitabashi M., Shinohara H., Yoshida S., Tsukiboshi T., Negishi H., Suyama K., Tsushima S. 2007a. Phylogenetic diversities of dominant culturable Bacillus, Pseudomonas and Pantoea species on tomato leaves and their possibility as biological control agents. Journal of Phytopathology 155 (7–8): 446–453. DOI: https://doi.org/10.1111/j.1439....
9.
Enya J., Shinohara H., Yoshida S,. Tsukiboshi T., Negishi H., Suyama K., Tsushima S. 2007b. Culturable leaf-associated bacteria on tomato plants and their potential as biological control agents. Microbial Ecology 53 (4): 524–356. DOI: 10.1007/s00248-006-9085-1.
10.
Ezra D., Barash I., Valinsky L., Manulis S. 2000. The dual function in virulence and host-range restriction of a gene isolated from the pPATHEhg plasmid of Erwinia herbicola pv. gypsophilae. Molecular Plant-Microbe Interactions 13: 683–692. DOI: https://doi.org/10.1094/MPMI.2....
11.
Galach’yan RM. 1961. The ways of infection of beet gall. p. 41–52. In:. “Problems of Microbiology I”. [Voprosy mikrobiologii. I.] (A.K. Panosyan, ed.). Mikrobiol. Sborn. Akad. Nauk. Armyan. S.S.R. Erevan:, Akad. Nauk Armyan. S.S.R.
12.
Goto K., Omura T., Hara Y., Sadaie Y. 2000. Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus, The Journal of General and Applied Microbiology 46 (1): 1–8. DOI: 10.2323/jgam.46.1.
13.
Harveson R.M., Hanson L.E., Hein G.L. 2009. Compendium of Beet Diseases and Pests. 2nd. ed., The American Phytopathological Society, St. Paul, USA, 140 pp. DOI: https://doi.org/10.1017/S00218....
14.
Huang X.F., Zhou D., Guo J., Manter D.K., Reardon K.F., Vivanco J.M. 2014. Bacillus spp. from rainforest soil promote plant growth under limited nitrogen conditions. Journal of Applied Microbiology 118 (3): 672–684. DOI: 10.1111/jam.12720.
15.
Huang X., Yong X., Zhang R., Shen Q., Yang X. 2013. The supernatant of Bacillus pumilus SQR-N43 has antifungal activity towards Rhizoctonia solani. Journal of Basic Microbiology 53 (8): 657–663. DOI: https://doi.org/10.1002/jobm.2....
16.
Liu Y., Lai Q., Du J., Shao Z. 2016. Bacillus zhangzhouensis sp. nov. and Bacillus australimaris sp. nov. International Journal of Systematic and Evolutionary Microbiology 66: 1193–1199. DOI: 10.1099/ijsem.0.000856.
17.
Kozyrovskaya N.A., Gvozdyak R.I., Muras V.A., Kordyum V.A. 1984. Changes in properties of phytopathogenic bacteria effected by plasmid pRD1. Archives of Microbiology 137 (4): 338–343. DOI: 10.1007/BF00410731.
18.
Kumar S., Stecher G., Li M., Knyaz C., Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35 (6): 1547–1549. DOI: 10.1093/molbev/msy096.
19.
Lazarev A.M. 2003-2009. Xanthomonas beticola. Interactive Agricultural Ecological Atlas of Russia and Neighboring Countries. Economic Plants and their Diseases, Pests and Weeds. Available on: http://www.agroatlas.ru/en/con... [Accessed: May 2023].
20.
LIZ 2023. Xanthomonas beticola. Available on: https://www.int-koop.de/schaed... [Accessed: May 2023].
21.
Manulis S., Haviv-Chesner A., Brandl M.T., Lindow S.E., Barash I. 1998. Differential involvement of indole-3acetic acid biosynthetic pathways in pathogenicity and epiphytic fitness of Erwinia herbicola pv. gypsophilae. Molecular Plant-Microbe Interactions 11 (7): 634–642. DOI: 10.1094/MPMI.1998.11.7.634.
22.
Moliszewska E., Nabrdalik M., Puławska J., Piszczek J. 2018. Tubercle disease of sugar beet roots (Beta vulgaris) found in Poland is neither caused by Xanthomonas beticola nor by tumorigenic Agrobacterium/Rhizobium. Journal of Plant Diseases and Protection 125: 581–583. DOI: https://doi.org/10.1007/s41348....
23.
Moliszewska E., Nabrdalik M., Piszczek J. 2016. Tubercle disease (Xanthomonas beticola) and other gall-malformed diseases of sugar beet roots: a review. Journal of Plant Diseases and Protection 123: 197–203. DOI: 10.1007/s41348-016-0032-1.
24.
Moliszewska E.B., Nabrdalik M. 2020. Application and biological impact of endophytic bacteria as IAA producers. p. 77–78. In: “Molecular Aspects of Plant Beneficial Microbes in Agriculture” (V. Sharma, R. Salwan, L.K.T Al-Ani, eds.). Elsevier, Academic Press, 438 pp. DOI: https://doi.org/10.1016/B978-0....
25.
Nabrdalik M., Moliszewska E., Wierzba S. 2018. Importance of endophytic strains Pantoea agglomerans in the biological control of Rhizoctonia solani. Ecological Chemistry and Engineering S 25 (2): 331–342. DOI: 10.1515/eces-2018-0023.
26.
Nyvall R.F. 1989. Field Crop Diseases Handbook. 2nd ed. Van Nostrand Reinhold, Springer New York, USA, 817 pp. DOI: https://doi.org/10.1007/978-1-....
27.
Patil V.S. 2019. Isolation of indole acetic acid producing bacteria from digester effluent and their effect on plant growth promotion. International Research Journal of Biological Sciences 8 (1): 1–9.
28.
Sari E., Etebarian H.R., Aminian H. 2007. The Effects of Bacillus pumilus, isolated from wheat rhizosphere, on resistance in wheat seedling roots against the take-all fungus, Gaeumannomyces graminis var. tritici. Journal of Phytopathology 155 (11–12): 720–727. DOI: https://doi.org/10.1111/j.1439....
29.
Sherf A.F., MacNab A.A. 1986. Vegetable Diseases and Their Control. 2nd ed. John Wiley & Sons, New York, USA, 728 pp.
30.
Starr M.P., Stephens W.L. 1964. Pigmentation and taxonomy of the genus Xanthomonas. Journal of Bacteriology 87 (2): 293–302. DOI: 10.1128/jb.87.2.293-302.1964.
31.
Szkop M., Sikora P., Orzechowski S. 2012. A novel, simple, and sensitive colorimetric method to determine aromatic amino acid aminotransferase activity using the Salkowski reagent. Folia Microbiologica (Praha) 57 (1): 1–4. DOI: 10.1007/s12223-011-0089-y.
32.
Tamura K., Nei M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10 (3): 512–526. DOI: 10.1093/oxfordjournals.molbev.a040023.
33.
Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J. 1996. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiological Reviews 60 (2): 407–438. DOI: 10.1128/mr.60.2.407-438.1996.
34.
Weinthal D.M., Barash I., Panijel M., Valinsky L., Gaba V., Manulis-Sasson S. 2007. Distribution and replication of the pathogenicity plasmid pPATH in diverse populations of the gall-forming bacterium Pantoea agglomerans. Applied and Environmental Microbiology 73 (23): 7552–7561. DOI: 10.1128/AEM.01511-07.
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