ORIGINAL ARTICLE
The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying
1
Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection – National Research Institute,
Poznań, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-03-03
Acceptance date: 2024-05-17
Online publication date: 2024-11-20
Corresponding author
Joanna Krzymińska
Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection – National Research Institute, Poznań, Poland
Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection – National Research Institute, Poznań, Poland
Journal of Plant Protection Research 2024;64(4):362-372
HIGHLIGHTS
- MG and trehalose preserved yeast's antagonistic abilities during freeze-drying.
- Treatment efficacy depended on isolate, supplements and timing of the treatment.
- The interplay between those factors affected the outcome.
- Isolate 117/10 was the most effective, MG and trehalose preserved its properties.
- Preventive treatments were generally more effective than intervention.
KEYWORDS
TOPICS
ABSTRACT
In the present eco-conscious era, consumers opt for food choices reflecting ethical and environmental concerns, which increases the demand for organic products. Biocontrol is
a viable plant protection method in organic farming. Freeze-drying is a long-term preservation
technique for microbial agents, ensuring their genetic stability and viability. To reduce
freeze-drying-induced damage to their cells, cryoprotective agents like trehalose and
monosodium glutamate are used. This study evaluated the impact of the addition of these
substances during the freeze-drying process on chosen yeast isolates’ viability, their ability
to survive on tomato leaves and maintain antagonistic properties against Botrytis cinerea
Pers. Yeast isolates 114/73 (Wickerhamomyces anomalus E.C. Hansen) and 117/10 (Naganishia
albidosimilis Vishniac & Kurtzman) were tested on tomato plants under greenhouse
conditions before and after the freeze-drying process for both the ability to colonize leaves
and as a preventive and interventional treatment against B. cinerea. Yeast viability post
freeze-drying was evaluated in vitro. Both trehalose and monosodium glutamate increased
yeast viability during the freeze-drying process. Viability was not very high (from 30.33 to
36.17% for 117/10 and from 10.67 to 16.5% for 114/73). Yeast dehydrated after freeze-drying,
protected with trehalose and monosodium glutamate, displayed the same colony count
on tomato leaves as before freeze-drying. The efficacy of protective treatments depended
on the yeast isolate, the protective substance used during freeze-drying, treatment timing
(prevention vs. intervention), and interactions of those factors. Cryopreserved isolate
117/10 performed better than 114/73 with the addition of either trehalose or monosodium
glutamate, reducing the disease severity index from 88.3% (control) to 18.75−55.33%. Preventive
treatments were more efficient than intervention. The leaf colonization ability and
biocontrol efficacy of yeast isolates against B. cinerea post-freeze-drying offer promising
solutions in sustainable agriculture. However, further research, to analyze the interactions
between various factors and to optimize strategies may be needed.
RESPONSIBLE EDITOR
Anna Baturo-Cieśniewska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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