ORIGINAL ARTICLE
The occurrence of three species of the genus Oscheius Andrássy, 1976 (Nematoda: Rhabditida) in Iran
1
Department of Entomology, Arak Branch, Islamic Azad University, Arak, Iran
2
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Submission date: 2016-12-31
Acceptance date: 2017-08-28
Corresponding author
Shila Goldasteh
Department of Entomology, Arak Branch, Islamic Azad University, Arak, Iran
Department of Entomology, Arak Branch, Islamic Azad University, Arak, Iran
Journal of Plant Protection Research 2017;57(3):248-255
KEYWORDS
Bayesian posterior probabilityITSmolecular analysisOscheius necromenusOscheius oniriciOscheius tipulae
TOPICS
ABSTRACT
Due to importance and effectiveness of some entomopathogenic or insect parasitic nematodes in controlling of agricultural pests, or pests of non-agricultural plants, a study was conducted in order to identify the species of this group of nematodes in city of Tehran. As the result, three species belonging to the genus Oscheius were recovered in association with bark samples having the bark beetle galleries. Morphological and molecular data were provided for two recently recovered species of the genus, namely O. necromenus and O. onirici. Molecular data were also provided for a recently recovered isolate of O. tipulae. All three species were recovered in association with bark samples collected from dead trees in the city of Tehran. Morphological characters and morphometric data of the two aforementioned species are in accordance with the data given in their original descriptions. One recovered individual from a small bark sample characterized by its short body length was sequenced for its 28S and internal transcribed spacer (ITS) rDNA loci, and the results of BLAST search using the newly obtained partial sequences revealed that it belonged to O. tipulae. Molecular phylogenetic studies revealed recently sequenced Iranian populations of O. onirici and O. tipulae forming a clade with other isolates/populations of these species in ITS tree with maximal Bayesian posterior probability (BPP), and presently sequenced isolates of O. tipulae and O. necromenus form a clade with other isolates of these species in 28S tree. The two species O. onirici and O. necromenus were reported in Iran for the first time.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (50)
1.
Aliramaji F., Pourjam E., Atighi M.R., Pedram M. 2014a. Devibursaphelenchus kheirii sp. n. (Nematoda: Ektaphelenchinae) from Iran with remarks on Devibursaphelenchus Kakuliya, 1967. Nematology 16 (9): 1069–1078. DOI: https://doi.org/10.1163/156854....
2.
Aliramaji F., Pourjam E., Atighi M.R., Roshan-Bakhsh A., Ye W., Pedram M. 2014b. Description of Ektaphelenchoides poinari sp. n. (Nematoda: Ektaphelenchinae) from Iran with compendium of the valid species of the genus Ektaphelenchoides Baujard, 1984. Russian Journal of Nematology 22: 11–22.
3.
Aliramaji F., Pourjam E., Karegar A., Atighi M.R., Pedram M. 2015. Description of Ektaphelenchoides caspiensis n. sp. (Nematoda: Ektaphelenchinae) from Iran. Nematology 17 (2): 169–177. DOI: https://doi.org/10.1163/156854....
4.
Alvani S., Mahdikhani-Moghadam E., Giblin-Davis R.M., Pedram M. 2016. Description of Ektaphelenchus berbericus n. sp. (Rhabditida: Ektaphelenchinae) from eastern Iran. Nematology 18 (9): 1063–1077. DOI: https://doi.org/10.1163/156854....
5.
Andrássy I. 1976. Evolution as a Basis for the Systematization of Nematodes. Pitman Publishing, Budapest, London, San Fransisco, 288 pp.
6.
Andrássy I. 2005. Free Living Nematodes of Hungary. Vol. 1: Nematoda errantia. Hungarian Natural History Museum, Budapest, 495 pp.
7.
Atighi M.R., Pourjam E., Pedram M., Ye W., Robbins R.T. 2012. Molecular and morphological characterization of Ektaphelenchoides kelardashtensis sp. n. (Nematoda: Ektaphelenchinae) from northern Iran. Russian Journal of Nematology 21: 23–30.
8.
Campos-Herrera R., Půža V., Jaffuel G., Blanco-Pérez R., Čepulyte-Rakauskiene R., Turlings T. 2015. Unraveling the intraguild competition between Oscheius spp. nematodes and entomopathogenic nematodes: implications for their natural distribution in Swiss agricultural soils. Journal of Invertebrate Pathology 132: 216–227. DOI: https://doi.org/10.1016/j.jip.....
9.
Castresana J. 2000. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17 (4): 540–552. DOI: https://doi.org/10.1093/oxford....
10.
Darsouei R., Karimi J., Shokoohi E. 2014. Oscheius rugaoensis and Pristionchus maupasi, two new records of entomophilic nematodes from Iran. Russian Journal of Nematology 22 (2): 141–55.
11.
De Grisse A.T. 1969. Redescription ou modifications de quelques techniques utilisées dans l’étude des nematodes phytoparasitaires. [Redescription or modification of some techniques used in the study of phytoparasitic nematodes]. Mededelingen Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 34: 351–369. (in French).
12.
Drummond A.J., Rambaut A. 2007. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology 7, 214 pp. DOI: https://doi.org/10.1186/1471-2....
13.
Dichtel-Danjoy M.L., Félix M.A. 2004a. Phenotypic neighborhood and micro-evolvability. Trends in Genetics 20 (5): 268–276. DOI: https://doi.org/10.1016/j.tig.....
14.
Dichtel-Danjoy M.L., Félix M.A. 2004b. The two steps of vulval induction in Oscheius tipulae CEW1 recruit common regulators including a MEK kinase. Developmental Biology 265 (1): 113–126. DOI: https://doi.org/10.1016/j.ydbi....
15.
Edgar R.C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32 (5): 1792–1797. DOI: https://doi.org/10.1093/nar/gk....
16.
Félix M.A., Vierstraete A., Vanfleteren J. 2001. Three biological species closely related to Rhabditis (Oscheius) pseudodolichura Körner in Osche, 1952. Journal of Nematology 33 (2–3): 104–109. PMCID: PMC2638136.
17.
Ghaemi R., Pourjam E., Álvarez-Ortega S., Pedram M., Atighi M.R. 2015. A curious nematode, Diploscapteroides persicus n. sp. (Nematoda: Rhabditida: Rhabditomorpha) from Iran with note on status of male genital papillae. Nematology 17 (9): 1045–1056. DOI: https://doi.org/10.1163/156854....
18.
Hassani-Kakhki M., Karimi J., Hosseini M., Heydari Sh. 2012. Efficacy of entomopathogenic nematodes against potato tuber moth, Phthorimaea operculella Zeller (Lep.: Gelechiidae). 20th Iranian Plant Protection Congress, Shiraz University, Shiraz, Iran, 25–28 August 2012, 987 pp.
19.
Huson D.H., Scornavacca C. 2012. Dendroscope 3: an interactive tool for rooted phylogenetic trees and networks. Systematic Biology 61 (6): 1061–1067. DOI: https://doi.org/10.1093/sysbio....
20.
Joyce S.A., Reid A., Driver F., Curran J. 1994. Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes. p. 178–187. In: “Biotechnology: Genetics of Entomopathogenic Nematode–Bacterium Complexes” (A.M. Burnell, R.U. Ehlers, J.P. Masson, eds.) Proceedings of Symposium and Workshop, St. Patrick’s College, Maynooth, Co. Kildare, Ireland, Luxembourg, European Commission, DG XII.
21.
Lam A.B.Q., Webster J.M. 1971. Morphology and biology of Panagrolaimus tipulae n. sp. (Panagrolaimidae) and Rhabditis (Rhabditella) tipulae n. sp. (Rhabditidae), from leatherjacket larvae, Tipula paludosa (Diptera: Tipulidae). Nematologica 17 (2): 201–212. DOI: https://doi.org/10.1163/187529....
22.
Larget B., Simon D.L. 1999. Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16 (6): 750–759. DOI: https://doi.org/10.1093/oxford....
23.
Liu Q.Z., Mrácčk Z., Zhang L.J., Půža V., Dong L.M. 2012. Redescription of Oscheius chongmingensis (Nematoda: Rhabditidae) and its entomopathogenicity. Nematology 14 (2): 139–149. DOI: https://doi.org/10.1163/138855....
24.
Miraeiz E., Heydari R., Álvarez-Ortega S., Pedram M., Atighi M.R. 2015. Molecular and morphological characterization of Veleshkinema iranicum n. gen., n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Iran. Zootaxa 4000 (5): 531–546.
25.
Nunn G.B. 1992. Nematode molecular evolution. An investigation of evolutionary patterns among nematodes based on DNA sequences. Ph.D. thesis, Nottingham, University of Nottingham, UK.
26.
Nylander J.A. 2004. MrModeltest v2. Evolutionary Biology Centre, Uppsala University. Available on: https://github.com/nylander/Mr.... Accessed [March 15, 2015].
27.
Pedram M., Pourjam E., Ye W., Atighi M.R., Robbins R.T., Ryss A. 2011. Description of Bursaphelenchus mazandaranense sp. n. (Nematoda: Parasitaphelenchidae) from Iran. Russian Journal of Nematology 19: 121–129.
28.
Pedram M., Pourjam E., Atighi M.R., Ye W., Houshmand A. 2012. Description of Ektaphelenchoides sylvestris sp. nov. (Nematoda: Ektaphelenchinae) from Iran. Annales Zoologici 62 (2): 325–329. DOI: https://doi.org/10.3161/000345....
29.
Pedram M., Pourjam E., Atighi M.R., Panahandeh Y. 2015. Further studies on soil nematode fauna in north western Iran with the description of one new species. Journal of Nematology 47 (2): 148–152. PMCID: PMC4492290.
30.
Shahabi S., Kheiri A., Rakhshandehroo F., Jamali S. 2016. Occurrence and distribution of nematodes in rice fields in Guilan province, Iran and the first record of Mylonchulus polonicus (Stefanski, 1915) Cobb, 1917 (Nematoda: Mononchina). Journal of Plant Protection Research 56 (4): 420–429. DOI: https://doi.org/10.1515/jppr-2....
31.
Ronquist F., Huelsenbeck J.P. 2003. MrBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 (12): 1572–1574. DOI: https://doi.org/10.1093/bioinf....
32.
Smart G.C. Jr, Nguyen K.B. 1994. Rhabditis (Oscheius) pheropsophi n. sp. (Rhabditida: Rhabditidae). Journal of Nematology 26 (1): 19–24. PMCID: PMC2619479.
33.
Soleymanzadeh M., Pedram M., Pourjam E., Álvarez-Ortega S. 2016. Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family. Nematology 18 (8): 987–998. DOI: https://doi.org/10.1163/156854....
34.
Sommer R.J. 2000. Comparative genetics: A third model nematode species. Current Biology 10 (23): 879–881. DOI: https://doi.org/10.1016/s0960-....
35.
Sudhaus W. 1976. Vergleichende Untersuchungen zur Phylogenie, Systematik, Ökologie, Biologie und Ethologie der Rhabditidae (Nematoda). [Comparative studies on the phylogeny, systematics, ecology, biology and ethology of the Rhabditidae (Nematoda)]. Zoologica 43: 1–228. (in German).
36.
Sudhaus W. 2011. Phylogenetic systemization and catalogue of paraphyletic “Rhabditidae” (Secernentea: Nematoda). Journal of Nematode Morphology and Systematics 14: 113–178.
37.
Sudhaus W. 2016. The guild of saprobiontic nematodes associated with ants. Ecologica Montenegrina 7: 600–613.
38.
Sudhaus W., Hooper D.J. 1994. Rhabditis (Oscheius) guentheri sp. n., an unusual species with reduced posterior ovary, with observations on the Dolichura and Insectivora groups (Nematoda: Rhabditidae). Nematologica 40 (1): 508–533. DOI: https://doi.org/10.1163/003525....
39.
Sudhaus W., Schulte F. 1989. Rhabditis (Rhabditis) necromena sp. n. (Nematoda: Rhabditidae) from South Australian Diplopoda with notes on its siblings R. myriophila Poinar, 1986 and R. caulleryi Maupas, 1919. Nematologica 35 (1): 15–24. DOI: https://doi.org/10.1163/002825....
40.
Tabassum K.A., Shahina F., Nasira K., Erum Y.I. 2016. Description of six new species of Oscheius Andrássy, 1976 (Nematoda: Rhabditida) from Pakistan with a key and diagnostic compendium to species of the genus. Pakistan Journal of Nematology 34 (2): 109–161. DOI: https://doi.org/10.18681/pjn.v....
41.
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725–2729. DOI: https://doi.org/10.1093/molbev....
42.
Torrini G., Mazza G., Carletti B., Benvenuti C., Roversi P.F., Fanelli E., De Luca F., Troccoli A., Tarasco E. 2015. Oscheius onirici sp. n. (Nematoda: Rhabditidae): a new entomopathogenic nematode from an Italian cave. Zootaxa 3937 (3): 533–548. DOI: https://doi.org/10.11646/zoota....
43.
Torrini G., Mazza G., Strangi A., Barabaschi D., Landi S., Mori E., Menchetti M., Sposimo P., Giuliani C., Zoccola A., Lazzaro L., Ferretti G., Foggi B., Roversi P.F. 2016. Oscheius tipulae in Italy: evidence of an alien isolate in the Integral Natural Reserve of Montecristo Island (Tuscany). Journal of Nematology 48 (1): 8–13. DOI: https://doi.org/10.21307/jofne....
44.
Whitehead A.G., Hemming J.R. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology 55 (1): 25–38. DOI: https://doi.org/10.1111/j.1744....
45.
Williams B.D., Schrank B., Huynh C., Shownkeen R., Waterston R.H. 1992. A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131 (3): 609–624. PMCID: PMC1205034.
46.
Wouts W., Mráček M.Z., Gerdin S., Bedding R.A. 1982. Neoaplectana Steiner, 1929 a junior synonym of Steinernema Travassos, 1927 (Nematoda: Rhabditida). Systematic Parasitology 4 (2): 147–154. DOI: https://doi.org/10.1007/bf0001....
47.
Yaghoubi A., Pourjam E., Pedram M., Siddiqi M.R., Atighi M.R. 2014a. Molecular and morphological characterization of Abursanema iranicum n. gen., n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Iran. Zootaxa 3826 (2): 301–314. DOI: https://doi.org/10.11646/zoota....
48.
Yaghoubi A., Pourjam E., Atighi M.R., Pedram M. 2014b. Molecular and morphological characterization of Ektaphelenchoides ruehmi sp. n. (Nematoda: Ektaphelenchinae) from south-western Iran. Russian Journal of Nematology 22: 31–38.
49.
Zhang K.Y., Liu X.H., Tan J., Wang Y., Qiao L., Yedid G., Dai C.S., Qiu R.L., Yan X.W., Tan H.W., Su Z.Y., Lai R., Gao G.F. 2012. Heterorhabditidoides rugaoensis n. sp. (Rhabditida: Rhabditidae), a novel highly pathogenic entomopathogenic nematode member of Rhabditidae. Journal of Nematology 44: 348–360.
50.
Zheng J., Subbotin S.A., Waeyenberge L., Moens M. 2000. Molecular characterisation of Chinese Heterodera glycines and H. avenae populations based on RFLPs and sequences of rDNA-ITS regions. Russian Journal of Nematology 8: 109–113.
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
