ORIGINAL ARTICLE
The influence of long-term storage in liquid nitrogen on survival and pathogenicity of Phytophthora infestans isolates
1
Plant Breeding and Acclimatization Institute – National Research Institute
Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland
Submission date: 2012-04-04
Acceptance date: 2012-10-24
Corresponding author
Jadwiga Śliwka
Plant Breeding and Acclimatization Institute – National Research Institute Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland
Plant Breeding and Acclimatization Institute – National Research Institute Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland
Journal of Plant Protection Research 2012;52(4):479-485
KEYWORDS
TOPICS
ABSTRACT
A set of 14 aggressive
Phythophthora infestans
(Mont.) de Bary isolates with a wide range of virulence, was stored for eight
years (2002–2010) in liquid nitrogen at –196°C (209 samples) and under paraffin oil at 7°C (70 test tubes). The survival rate of samples
stored in liquid nitrogen was scored as 88%, 45 days after thawing. The revived isolates were passaged through potato tissues four
times and tested for virulence on a set of 11 Black’s differentials. The mean number of virulence factors per isolate, of these
P. infestans
isolates evaluated before storage, was 7.4. The isolates stored in liquid nitrogen and under paraffin oil and passaged four times
through potato tissues showed a similar mean number of virulence factors per isolate, 7.3 and 6.9, respectively. Isolates stored under
paraffin oil showed no expression of factor
avr5
, but expression of this factor occurred in six isolates after liquid nitrogen treatment.
The initial expression of this factor occurred in four isolates. Before storage, the average aggressiveness of the tested isolates was assessed as 1.7, on a 1–9 scale, where 1 means the most aggressive. After storage and after four subsequent passages through potato tissues, the mean aggressiveness of isolates stored in liquid nitrogen and under paraffin oil reached the level of 1.5 and 2.1, respectively.
The mean aggressiveness was not significantly different from the level of initial assessment.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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