ORIGINAL ARTICLE
The feeding behavior of Diaphorina citri monitored by using an electrical penetration graph (DC-EPG) on citrus plants treated with Bacillus cereus and Bacillus velezensis
1
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
2
Laboratory of Insect Ecology, South China Agricultural University, Guangzhou, China
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-01-10
Acceptance date: 2024-03-06
Online publication date: 2024-09-10
Corresponding author
Tri Joko
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Journal of Plant Protection Research 2024;64(3):234-241
HIGHLIGHTS
- Candidatus Liberibacter asiaticus, a phloem-limited bacteria, was transmitted by the Asian citrus psyllid Diaphorina citri
- The effect of Bacillus on citrus plant growth and the feeding behavior of Diaphorina citri was evaluated
- The application of Bacillus cereus and Bacillus velezensis enhanced plant resistance to stylet penetration of Diaphorina citri on the citrus phloem tissues
KEYWORDS
TOPICS
ABSTRACT
Diaphorina citri, an important pest and insect vector that can transmit the pathogenic bacteria
Candidatus Liberibacter asiaticus, causing Huanglongbing disease, is one of many
challenges in citrus agriculture. Integrated pest management by utilizing microorganisms
is a wise and efficient alternative without damaging the environment. Utilization of Plant
Growth Promoting Rhizobacteria (PGPR), such as Bacillus cereus and B. velezensis, is
a potential strategy for the biological control of plant diseases or insect vectors. By inducing
systemic resistance in plants, PGPR can enhance plant defense against diseases and insect
pests while activating molecular and physiological changes in plants. This research aimed
to determine the effect of B. cereus and B. velezensis on the plant growth and feeding behavior
of D. citri. The height and volume of the plant canopy were observed periodically for
6 months, while the feeding behavior of D. citri was monitored using the Electrical Penetration
Graph (DC-EPG). The results showed increased height and volume of the citrus plant
canopy treated with B. cereus, indicating that B. cereus could act as a PGPR. The application
of B. cereus and B. velezensis to citrus seedlings affected the feeding behavior of D. citri.
D. citri showed difficulty in penetrating the phloem tissue of citrus plants.
FUNDING
This work was supported partly by the Australian
Center for International Agricultural Research (Grant
No. ACIAR HORT 2019/164) and Universitas Gadjah
Mada under the RTA Program (Grant No. 5075/
UN1.P.II/Dit-Lit/PT.01.01/2023). This manuscript is
part of the thesis by IM under the guidance of TJ and
the team.
RESPONSIBLE EDITOR
Piotr Iwaniuk
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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