In the years 2004–2006 with winter rye was conducted the research, both in a field experiment and in laboratory conditions, on the effect of chlorsulfuron (Glean 75 WG) and iodosulfuron methyl-sodium + mesosulfuron methyl (Atlantis 04 WG), used in 3–4 leaf stage in the autumn and plants at full tillering in the spring, on yielding and grain quality of two hybrid cultivars; Fernando F1 and Stach F1. The results obtained proved that winter rye, cultivated on black soil of the IIa class, produced high yield regardless plant cultivar, as well as applied herbicides which did not have a phytotoxic effect on plant growth and development. Neither the yield nor mass of 1000 grains was essentially affected by the use of herbicides or a date of their application. The mentioned parameters varied due to the course of weather conditions during the plant growing season. The examined herbicides did not significantly affect such characteristics as: protein content, falling number, equalization and density of grain.