ORIGINAL ARTICLE
Temperature and pH impact on the mycelium growth of Mycogone perniciosa and Verticillium fungicola isolates derived from Polish and foreign mushroom growing houses
1
Poznań University of Life Sciences
Department of Vegetable Crops, Dąbrowskiego 159, 60-594 Poznań, Poland
2
Poznań University of Life Sciences
Department of Plant Protection Methods, Zgorzelecka 4, 60-198 Poznań, Poland
Corresponding author
Marek Siwulski
Poznań University of Life Sciences Department of Vegetable Crops, Dąbrowskiego 159, 60-594 Poznań, Poland
Poznań University of Life Sciences Department of Vegetable Crops, Dąbrowskiego 159, 60-594 Poznań, Poland
Journal of Plant Protection Research 2011;51(3):268-272
KEYWORDS
TOPICS
ABSTRACT
The research project aimed at determining the effect of incubation temperature and pH of the agar medium on the mycelium
growth of
Mycogone perniciosa
(Magnus) Delacroix and
Verticillium fungicola
(Preuss) Hassebrauk in
in vitro
conditions. Ten
M. perni-
ciosa
and ten
V. fungicola
isolates were applied. Mycelium growth of the above-mentioned isolates was studied on PDA agar medium
(Merck) enriched with an extract from mushroom hyphae, at temperatures ranging from 15 to 30°C and pH from 5.5 to 7.5. Incubation temperature was found to affect the mycelium growth rate of the examined pathogens. Mycelium growth in all
M. perniciosa
and
V. fungicola
isolates was the fastest at the temperature of 25°C, while a temperature of 15°C inhibited their growth significantly. Mycelium growth of the examined pathogens also depended on medium pH.
M. perniciosa
mycelium developed best at pH = 5.5, whereas
at the same medium pH
V. fungicola
mycelium exhibited the worst growth.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (17)
1.
Bech K., Jacobsen B.D., Kovacs G., Grabbe K., Hilber O. 1989. Investigations on the influence of temperature on growth and spore formation of Mycogone perniciosa and Verticillium fungicola, two pathogenic fungi of the cultivated mushroom. Mush. Sci. 12 (2): 739–751.
2.
Fletcher J.T., Gaze R.H. 2008. Mushroom Pest and Disease Control. Manson Publishing, London, UK, 192 pp.
3.
Gams W., van Zaayen A. 1982. Contribution to the taxonomy and pathogenicity of fungicolous Verticillium species. I. Taxonomy. Neth. J. Plant Pathol. 88 (2): 57–78.
4.
Gea F.J., Tello J.C., Navarro M.J. 2003. Occurrence of Verticillium fungicola var. Fungicola on Agaricus bitorquis mushroom crops in Spain. J. Phythopathol. 151 (2): 98–100.
5.
Geels F.P., van de Geijn J., Rutjens A.J. 1988. Pests and diseases. p. 361–422. In: “The Cultivation of Mushrooms” (L.J.LD. van Griensven, ed.). Horst, Netherlands, 515 pp.
6.
Glamoclija J., Sokovic M., Ljaljevic-Grbic M., Vukojevic J., Milenkovic I., van Griensven L. 2008. Morphological characteristics and mycelial compatibility of different Mycogone perniciosa isolates. J. Microsc. 232 (3): 489–492.
7.
Juarez del Carmen S., Largeteau-Mamoun M.L., Rousseau T., Regnault-Roger C., Savoie J.M. 2002. Genetic and physiological variation in isolates of Verticillium fungicola causing dry bubble disease of the cultivated button mushroom, Agaricus bisporus. Mycol. Res. 106 (8): 1163–1170.
8.
Lambert E.B. 1930. Studies on the relation of temperature to the growth, parasitism, thermal death points, and control of Mycogone perniciosa.Phytopathology 20 (1): 75–83.
9.
Maszkiewicz J., Dmowska E., Ignatowicz S., Lewandowski M., Szymański J. 2006. Ochrona Pieczarki. Praca zbiorowa pod red. J. Maszkiewicza. Hortpress, Warszawa, 144 pp.
10.
Meyer L., Korsten L. 2008. A nested PCR for the detection of Mycogone perniciosa causing wet bubble disease of white button mushrooms. Mush. Sci. 17 (1): 554–564.
11.
Nair N.G., Macauley B.J. 1987. Dry bubble disease of Agaricus Bisporus and A. bitorquis, and its control by prochlorazmanganese complex. New Zealand J. Agri. Res. 30 (1): 107–116.
12.
Sharma V.P., Singh Ch. 2003. Biology and control of Mycogone perniciosaMagn. causing wet bubble disease of white button mushroom. J. Mycol. Plant Pathol. 33 (2): 257–264.
13.
Tan Q., Wang l., Wang Y., Wang J. 1994. A study on biological characteristics in isolates of Mycogone perniciosa Magn. Acta Edulis Fungi 4 (2): 1996–2001.
14.
Thapa C.D., Jandaik C.L. 1986. Spore germination behavior of Verticillium fungicola(Preuss) Hassebr. under different environmental conditions. p. 405–410. In: Proc. Int. Symp. Sci. Technical Aspects Cult. of Edible Fungi, Pennsylvania State University, PA, USA, 15–17 July 1986.
15.
Umar M.H., Geels F.P., van Griensven L.J.LD. 2000. Pathology and pathogenesis of Mycogone perniciosa infection of Agaricus bisporus. p. 561–567. In: Proc. 15th Int. Cong. Sci. and Cult. of Edible Fungi, vol. 2, Maastricht, Netherlands. 15–19 May 2000.
16.
van Zaayen A., Gams W. 1982. Contribution to the taxonomy and pathogenicity of fungicolous Verticillium species. II. Pathogenicity. Neth. J. Plant Path. 88 (2): 143–154.
17.
Zare R., Gams W. 2008. A revision of the Verticillium fungicola species complex and its affinity with the genus Lecanicillium. Mycol. Res. 112 (7): 811–824.
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
