ORIGINAL ARTICLE
Systemic protection against pearl millet downy mildew disease induced by cell wall glucan elicitors from Trichoderma hamatum UOM 13
1
Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore – 570006, Karnataka, India
2
Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri, Mysore – 570006, Karnataka, India
3
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore – 570006, Karnataka, India
Submission date: 2016-10-21
Acceptance date: 2017-03-01
Corresponding author
Kestur Nagaraju Amruthesh
Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore – 570006, Karnataka, India
Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore – 570006, Karnataka, India
Journal of Plant Protection Research 2017;57(3):298-308
KEYWORDS
TOPICS
ABSTRACT
The obligate oomycete Sclerospora graminicola (Sacc.) Schroet, is the incitant of
downy mildew disease, which is the main constraint in pearl millet production
worldwide. Different elicitors from Trichoderma hamatum UOM 13, e.g. mycelial
extract and cell wall glucans, were assessed for their resistance elicitation efficiency
and the possible underlying mechanisms. Both mycelial extract and cell wall glucans
of T. hamatum UOM 13 positively influenced seed quality parameters of pearl millet,
significantly enhanced seed germination and seedling vigor in comparison to the
untreated control. Seed priming with cell wall glucan elicitors of T. hamatum UOM
13 suppressed downy mildew on susceptible pearl millet seedlings under greenhouse
conditions by induction of systemic host resistance. Of the different elicitor delivery
methods tested, transplant root dip was more effective than seed treatment and foliar
spray. A combination of transplant root dip + seed treatment + foliar spray was
significantly more effective than the single delivery methods. The induced resistance
corresponded to up regulation of genes of important defense proteins upon pathogen
inoculation. Transcripts of genes of defense enzymes glucanase, phenylalanine ammonia lyase, peroxidase and polyphenoloxidase were significantly increased due to
the T. hamatum UOM elicitor effect. Expression of hydroxyproline-rich glycoprotein
genes, known to play an important role in cell wall cross-linking, were also up regulated
in response to T. hamatum UOM cell wall glucan treatment. This study emphasizes
the role of T. hamatum UOM as a potential elicitor of downy mildew resistance in pearl millet and presents novel insights into the involvement of important defense proteins mediating such as resistance trigger.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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