ORIGINAL ARTICLE
Survey of postharvest fungi associated with sorghum grains produced in Karnataka (India)
1
Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 70 006, Karnataka, India
2
Molecular Phytodiagnostic Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India
Corresponding author
Janardhana Gottravalli Ramanayaka
Molecular Phytodiagnostic Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India
Molecular Phytodiagnostic Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India
Journal of Plant Protection Research 2010;50(3):335-339
KEYWORDS
TOPICS
ABSTRACT
Sorghum is an important cereal produced as staple diet in Karnataka state of India and is prone to fungal infection during pre- and postharvest period. This paper reports the frequency and relative percentage of fungi associated with sorghum grain harvested in Karnataka State in 2004 and 2005. A total of 44 sorghum samples were analyzed for postharvest fungi by direct plating
method on PDA and MGA 2.5 agar medium. The genera Fusarium and
Aspergillus were the most frequently isolated on sorghum grain. The other genera included Alternaria, Phoma, Curvularia, Penicillium and Drechslera. The data revealed a high frequency of Fusarium species (93.2%) and
Aspergillus species (88.6%) with a relative percentage of 23.3 and 19.6% among the 19 fungal genera recorded, respectively. The predominant fungal species recorded at high frequency were F. verticillioides (88.6%), A. flavus
(72.7%), F. anthophilum (65.0%), A. niger (59.1%). These data indicate possible health hazards for humans and animals upon consumption of such contaminated food grain by toxigenic moulds.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (34)
1.
Anonymous. 2001. Joint FAO/WHO Expert Committee on Food Additives. Summary and Conclusions, Fifty-sixth meeting, Geneva. 6–15 February 2001, USA, p. 19.
2.
Anonymous. 2003. Directorate of Economics and Statistics, Government of Karnataka, Bangalore, Karnataka, India. Available from: http://des.kar.nic.in/indexie.....
3.
Barnett H.L., Hunter B.B. 1972. Illustrated Genera of Imperfect Fungi. III ed. Burgess Publishing Company, Minneapolis, Minnesota, USA, 208 pp.
4.
Bhat R.V., Prathapkumar H.S., Rao P.A., Rao V.S. 1997. A food-borne disease outbreak due to the consumption of moldy sorghum and maize containing fumonisin mycotoxins. Clin. Toxicol. 35 (3): 249–255.
5.
Booth C. 1977. Fusarium: Laboratory Guide to the Identification of Major Species. Commonwealth Mycological Institute. Ferry Lane. Kew, Surrey, England: 4–57.
6.
Bragulat M.R., Martinez E., Castella G., Cabanes F.J. 2004. Selective efficacy of culture media recommended for isolation and enumeration of Fusarium species. J. Food Protect. 67 (1): 207–211.
7.
Castro M.F.P.M., Soares L.M.V., Furlani R.R.Z. 1995. Mycoflora, aflatoxigenic species and mycotoxins in freshly harvested corn (Zea mays L.): a preliminary study. Rev. Microbiol. 26: 289–295.
8.
Christensen C.M., Kaufmann H.H. 1969. Grain Storage: The Role of Fungi in Quality Loss. University of Minnesota Press, Minneapolis, USA: 36–108.
9.
Da Silva J.B., Dilkin P., Fonseca H., Correa B. 2004. Production of aflatoxin by Aspergillus flavus and fumonisin by Fusarium species isolated from Brazilian sorghum. Braz. J. Microbiol. 35 (3): 182–186.
10.
Dass R.S., Sreenivasa M.Y., Janardhana G.R. 2007. High incidence of Fusarium verticillioides in animal and poultry feed mixtures produced in Karnataka, India. Plant Pathol. J. 6 (2): 174–178.
11.
Diener U.L., Morgan-Jones G., Wagener R.E., Davis N.D. Toxigenicity of fungi from grain sorghum. Mycopathology 75 (1): 23–26.
12.
El-Maghraby O.M.O., El-Kady I.A., Soliman S. 1995. Mycoflora and Fusarium toxins of three types of corn grains in Egypt with special reference to production of trichothecene toxins. Microbiol. Res. 150 (3): 225–232.
13.
Gamanya R., Sibandal L. 2001. Survey of Fusarium verticillioides (F. monilioforme) and production of fumonisin B1 in cereal grains and oilseeds in Zimbabwe. Int. J. Food Microbiol. 71 (2–3): 145–149.
14.
Gassen M.A. 1999. Study of the microorganisms associated with the fermented bread (Khamir) produced from sorghum in Gizan region, Saudi Arabia. J. Appl. Microbiol. 86 (2): 221–225.
15.
Ghiasian S.A., Kord-Bacheh P., Rezayat S.M., Maghsood A.H., Taherkhani H. 2004. Mycoflora of Iranian maize harvested in the main production areas in 2000. Mycopathology 158 (1): 113–121.
16.
Gonzalez H.H.L., Martinez E.J., Resnik S.L. 1997. Fungi associated with sorghum grain from Argentina. Mycopathologia 139 (1): 35–41.
17.
Hulluka M., Esele J.P.E. 1992. Sorghum diseases in eastern Africa p 21–24. In: “Sorghum and Millets Diseases: A Second World Review” (W.J.A. de Milliano, R.A. Frederiksen, G.D. Bergston, eds.). ICRISAT, Patancheru, India.
18.
Janardhana G.R., Raveesha K.A., Shetty H.S. 1999. Mycotoxins contamination of maize grains grown in India (Karnataka). Food Chem. Toxicol. 37 (8): 863–868.
19.
Keith S. 1996. Fuskey-Fusarium Interactive Key. Agriculture and Agri-Food Canada, Canada. Available from: http://www.ctu.edu.vn/colleges....
20.
Kleih U., Bala Ravi S., Dayakar Rao B., Yoganand B. 2000. Industrial Utilization of Sorghum in India. p. 13 In: 4th International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Patancheru, AP, India.
21.
Klich M.A. 2007. Aspergillus flavus: the major producer of aflatoxin. Mol. Plant Pathol. 8 (6): 713–722.
22.
Leslie J.F., Pearson C.A.S., Nelson P.E., Toussoun T.A. 1990. Fusarium spp. from corn, sorghum, and soybean fields in the Central and Eastern United States. Phytopathology 80: 343–350.
23.
Leslie J.F., Summerell B.A. 2006. The Fusarium, Laboratory Manual. 1st ed. Blackwell Publishing Professional, USA, 274 pp.
24.
Marasas W.F.O., Nelson P.E., Toussoun T.A. 1984. Toxigenic Fusarium Species: Identity and Mycotoxicology. Pennsylvania State University Press, University Park, USA: 80–318.
25.
Mathur S.B., Kongsdal O. 2003. Common laboratory seed health-testing methods for detecting fungi. International Seed Testing Association, Switzerland: 234–255.
26.
Nelson P.E., Dignani M.C., Anassie E.J. 1994. Taxonomy, biology and clinical aspects of Fusarium species. Clin. Microbiol. Rev. 7 (4): 479–504.
27.
Ono E.Y.S., Sugiura Y., Homechin M., Kamogae M., Vizzoni E., Ueno Y., Hirooka E.Y. 1999. Effect of climatic conditions on natural mycoflora and fumonisins in freshly harvested corn of the State of Parana, Brazil. Mycopathology 147 (3): 139–148.
28.
Pitt J.I., Hocking A.D., Bhudhasamai K., Miscamble B.F., Wheele K.A., Tanboon-Ek P. 1994. The normal mycoflora of commodities from Thailand: beans, rice, small grains and other commodities. Int. J. Food Microbiol. 23 (1): 35–53.
29.
Placinta C.F., D’Mello J.P., MacDonald A.M. 1999. A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim. Feed Sci. Technol. 78: 21–37.
30.
Rheeder J.P., Marasas W.F.O. 1998. Fusarium species from plant debris associated with soils from maize production areas in the Transkei region of South Africa. Mycopathologia 143 (2): 113–119.
31.
Singh K., Frisrad J.C., Thrane U., Mathur S.B. 1999. An Illustrated Manual on Identification of Some Seed-Borne Aspergilli, Fusaria, Penicillia and their Mycotoxins. Danish Govt. Institute of Seed Pathology for Developing Countries, Denmark: 6–122.
32.
Summerell B.A., Salleh B., Leslie J.F. 2003. A utilitarian approach to Fusarium identification. Plant Dis. 87 (2): 117–128.
33.
Williams R.J., McDonald D. 1983. Grain moulds in the tropics: problems and importance. Ann. Rev. Phytopathol. 21: 153–178.
34.
Wilson J.P., Cooper H.H., Wilson D.M. 1995. Effect of delayed harvest on contamination of pearl millet grain with mycotoxin producing fungi and mycotoxins. Mycopathology 132 (1): 27–30.
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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