ORIGINAL ARTICLE
Suppression of growth of Fusarium verticillioides Niren. using strains of Trichoderma harzianum from maize (Zea mays) plant parts and its rhizosphere
1
Department of Plant Science and Applied Zoology, Olabisi Onabanjo University, P.M.B. 2002, Ago-Iwoye, Ogun State, Nigeria
2
Department of Botany and Microbiology, University of Ibadan, Ibadan, Oyo-State, Nigeria
3800 9th St SW rm 3436, Washington DC 20024, USA
3
Pathology Unit, International Institute of Tropical Agriculture,
C
/o L.W. Lambourn (UK) Ltd, Carolyn House, 26 Dingwall Road,
Croydon CR9 3EE, UK,
Corresponding author
Ayodele Adegboyega Sobowale
Department of Plant Science and Applied Zoology, Olabisi Onabanjo University, P.M.B. 2002, Ago-Iwoye, Ogun State, Nigeria
Department of Plant Science and Applied Zoology, Olabisi Onabanjo University, P.M.B. 2002, Ago-Iwoye, Ogun State, Nigeria
Journal of Plant Protection Research 2009;49(4):452-459
KEYWORDS
TOPICS
ABSTRACT
Three strains of
Trichoderma harzianum
(strain 1: IMI 380934; strain 2: IMI 380935; strain 3: IMI 380938) were compared for
their ability to suppress radial growth of
Fusarium verticillioides
in vitro
. Each
Trichoderma
strain was paired with the pathogen
F. verti -
cillioides
on 9 cm Petri plates of acidified potato dextrose agar using three pairing methods. Varying growth suppression of pathogen
by
Trichoderma
strains was rated and ratings were analysed using GLM Procedure of SAS. Growth inhibition of
F. verticillioides
by each
of the
T. harzianum
strains was significantly different from control irrespective of pairing method (p = 0.01, R
2
= 0.96). Higher inhibition
of
F. verticillioides
was obtained by inoculating antagonist before pathogen even at p = 0.01. Mode of suppression includes mycoparasitism and competition for space and nutrients. Growth inhibition of pathogen differed significantly among (p > 0.0001) and within
(p > 0.026) pairing methods.
T. harzianum
strain 1 had better suppression of pathogen than the other two strains when it was inoculated
before the pathogen while
T. harzianum
strain 3 was better when pathogen and antagonist were inoculated simultaneously (p = 0.05).
Different strains of
T. harzianum
could thus be employed as promising antagonists of
F. verticillioides
.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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