ORIGINAL ARTICLE
Screening of antimicrobial and antioxidant secondary metabolites from endophytic fungi isolated from wheat (Triticum durum)
1
University Ferhat Abbas Sétif 1, Laboratory of Applied Microbiology, Faculty of Natural Sciences and Life Sétif 19000, Algeria
Submission date: 2012-08-31
Acceptance date: 2013-03-20
Corresponding author
Harzallah Daoud
University Ferhat Abbas Sétif 1, Laboratory of Applied Microbiology, Faculty of Natural Sciences and Life Sétif 19000, Algeria
University Ferhat Abbas Sétif 1, Laboratory of Applied Microbiology, Faculty of Natural Sciences and Life Sétif 19000, Algeria
Journal of Plant Protection Research 2013;53(2):128-136
KEYWORDS
TOPICS
ABSTRACT
The emergence of antibiotic-resistant micro-organisms calls for inventive research and development strategies. Inhibition of these pathogenic micro-organisms may be a promising therapeutic approach. The screening of antimicrobial compounds from
endophytes is a promising way to meet the increasing threat of drug-resistant strains of human and plant pathogens. In the present study, a total of 20 endophytic fungi and 23 endophytic actinomycetes have been isolated from wheat (Triticum durum). Mohamed Ben Bachir variety collected from Bordj Bou Arreridj region (Algeria) during winter 2010. The isolates were screened and evaluated for their antimicrobial and antioxidant activities. Antimicrobial activity was evaluated for crude ethyl acetate extracts using an agar diffusion assay against twelve pathogenic bacteria, yeast, and two phytopathogenic fungi. All extracts showed inhibitory activity on at least one or more pathogenic microorganisms, with an average zone of inhibition varied between 7 mm to 25 mm, and the largest zone was of 25 and 25.3 mm against candida albicans and Escherichia coli respectively. The antioxidant capacity of the extracts was evaluated by β-carotene/linoleic acid assay. Results showed that 60% of these extracts have antioxidant activity, exhibiting 50, 57% to 78, 96% inhibitions. While the inhibitory activity for oxidation of linoleic acid of 40% of them was less than 50%. From the present work it is possible to conclude that these microorganisms could be promising source of bioactive compounds, and warrant further study.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (64)
1.
Andrew J.H., Hirano S.S. 1991. Microbial Ecology of Leaves. Brock/Spinger Series, Springer Verlag, London, 516 pp.
2.
Baghiani A., Boumerfeg S., Belkhiri F., Khennouf S., Charef N., Harzallah D., Arrar L., Abdel-Wahhab M.A. 2010. Antioxidant and radical scavenging properties of Carthamus caeruleus L. extracts grow wild in Algeria flora. Comunicata Scientiae 1 (2): 128–136.
3.
Baris O., Gulluce M., Sahin F., Ozer H., Kilic H., Ozkan H., Sokmen M., Ozbek T. 2006. Biological activities of the essential oil and methanol extract of Achillea biebersteiniii Afan. (Asteraceae). Turk. J. Biol. 30: 65–73.
4.
Bernstein M.E., Carroll G.C. 1977. Internal fungi in old-growth Douglas fir foliage. Can. J. Bot. 55 (6): 644–653.
5.
Botton B., Breton A., Fevre M., Guy P.H., Iarpent J.P., Sanglier J.J., Vayssier V., Veau P. 1990. Moisissures Utiles et Nuisibles: Importance Industrielle. Masson, France, 512 pp.
6.
Cao L., Huang J., Li J. 2007. Fermentation conditions of Sinopodophyllum hexandrum endophytic fungus on production of podophyllotoxin. Food and Fermentation Industries 33: 28–32.
7.
Cao L.X., Qiu Z.Q., You J.L., Tan H.M., Zhou S. 2005. Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol. Lett. 247 (2): 147–152.
8.
Castillo U.F., Strobel G.A., Ford E.J., Hess W.M., Porter H., Jensen J.B., Albert H., Robison R., Condron M.A.M., Teplow D.B., Stevens D., Yaver D. 2002. Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562 endophytic on Kennedia nigriscans. Microbiology 148 (9): 2675–2685.
9.
Champion R. 1997. Identifier les Champignons Transmis par les Semences. Paris, INRA Edition, 401 pp.
10.
Chareprasert S., Piapukiew J., Thienhirun S., Whalley A., Sihanonth P. 2006. Endophytic fungi of teak leaves Tectona grandis L. and rain tree leaves Samanea saman Merr. World J. Microbiol. Biotechnol. 22: 481–486.
11.
Clay K. 1989. Clavicipitaceous endophytes of grasses: their potential as biocontrol agents. Mycol. Res. 92 (1): 1–12.
12.
Conde E., Moure A., Domınguez H., Parajo J.C. 2008. Fractionation of antioxidants from autohydrolysis of barley husks. J. Agric. Food Chem. 56 (22): 10651–10659.
13.
Dapkevicius A., Venskutonis R., van Beek T.A., Linssen P.H. 1998. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in lithuania. J. Sci. Food Agric. 77 (1): 140–146.
14.
Demain A.L. 2000. Microbial natural products: a past with a future. p. 3–16. In: “Biodiversity: New Leads for Pharmaceutical and Agrochemical Industries” (S.K. Wrigley, M.A. Hayes, R. Thomas, E.J.T. Chrystal, N. Nicholson, eds.). The Royal Society of Chemistry, Cambridge, United Kingdom, 313 pp.
15.
Dewan M.M., Sivasithamparam K. 1989. Behaviour of a plant growth promoting sterile fungus on agar and roots of ryegrass and wheat. Mycol. Res. 93 (2): 161–166.
16.
Fernandes M.R.V., Costa-Silva T.A., Pfenning L.H., Costa-Neto C.M., Heinrich T.A., Alencar S.M., Lima M.A., Ikegaki M. 2009. Biological activities of the fermentation extract of the endophytic fungus Alternaria alternata isolated from Coffea arabica L. Braz. J. Pharm. Sci. 45 (4): 677–685.
17.
Gao L., Huang J., Li J. 2007. Fermentation conditions of Sinopodophyllum hexandrum endophytic fungus on production of podophyllotoxin. Food and Fermentation Industries 33: 28–32.
18.
Gong L.J., Gou S.X. 2009. Endophytic fungi from Dracaena cambodiana and Aquilaria sinensis and their antimicrobial activity. Afr. J. Biotechnol. 8 (5): 731–736.
19.
Guo B., Wang Y., Sun X., Tang K. 2008. Bioactive natural products from endophytes: a review. Appl. Biochem. Microbiol. 44 (2): 136–142.
20.
Harper J.K., Arif A.M., Ford E.J., Strobel G.A., Porco J.A., Tomer Jr. D.P., Oneill K.L., Heider E.M., Grant D.M. 2003. Pestacin: a 1,3-dihydro isobenzofuran from Pestalotiopsis microspora possessing antioxidant and antimycotic activities. Tetrahedron 59 (14): 2471–2476.
21.
Hasegawa S., Meguro A., Shimizu M., Nishimura T., Kunoh H. 2006. Endophytic actinomycetes and their interactions with host plants. Actinomycetologica 20 (2): 72–81.
22.
Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Williams S.T. 1994. Bergey’s Manual of Determinative Bacteriology. 9th ed. Lippincott Williams & Wilkins, Baltimore, 619 pp.
23.
Huang W.Y., Cai Y.Z., Hyde K.D., Corke H., Sun M. 2008. Biodiversity of endophytic fungi assosiated with 29 traditional chinese medicinal plants. Fungal Divers. 33: 61–75.
24.
Igarashi Y., Iida T., Yoshida R., Furumai T. 2002. Pteridic acids A and B, novel plant growth promoters with auxin-like activity from Streptomyces hygroscopicus TP-A0451. J. Antibiot. 55 (8): 764–767.
25.
Khennouf S., Iratni N., Baghiani A., Harzallah D., Arrar L. 2010. Antioxidant and antibacterial activities of extracts from Artemisia herba alba Asso leaves and some phenolic compounds. J. Med. Plant Res. 4 (13): 1273–1280.
26.
Kour A., Shawl A.S., Rehman S., Sultan P., Qazi P.H., Suden P., Khajuria R.K., Verma V. 2008. Isolation and identification of an endophytic strain of Fusarium oxysporum producing podophyllotoxin from Juniperus recurva. World J. Microbiol. Biotechnol. 24: 1115–1121.
27.
Kumar S., Sagar A. 2007. Microbial associates of Hippophae rhamnoides (Seabuckthorn). Plant Pathol. J. 6 (4): 299–305.
28.
Kwon H.R., Son S.W., Han H.R., Choi G.J., Jang K.S., Choi Y.H., Lee S., Sunog N.D., Kim J.C. 2007. Nematicidal activity of bikaverin and fusaric acid isolated from Fusarium oxysporum against pine wood nematode Bursaphelenchus xylophilus. Plant Pathol. J. 23 (4): 318–321.
29.
Larran S., Perello A., Simon M.R., Moreno V. 2002. Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves. World J. Microbiol. Biotechnol. 18: 683–686.
30.
Larran S., Perello A., Simon M.R., Moreno V. 2007. The endophytic fungi from wheat (Triticum aestivum L.). World J. Microbiol. Biotechnol. 23: 565–572.
31.
Lee J., Lobkovsky E., Pliam N.B., Strobel G., Clardy J. 1995. Subglutinols A and B: immunosuppressive compounds from the endophytic fungus – Fusarium subglutinans. J. Org. Chem. 60 (22): 7076–7077.
32.
Lin X., Lu C.H., Huang Y.J., Zheng Z.H., Su W.J., Shen Y.M. 2007. Endophytic fungi from a pharmaceutical plant, Camptotheca acuminata: isolation, identification and bioactivity. World J. Microbiol. Biotechnol. 23: 1037–1040.
33.
Liu C.H., Zou W.X., Lu H., Tan R.X. 2001. Antifungal activity of Artemisia annua endophyte cultures against phytopathogenic fungi. J. Biotechnol. 88 (3): 277–282.
34.
Lv Y.L., Zhang F.S., Chen J., Cui J.L., Xing Y.M., Li X.D., Guo S.X. 2010. Diversity and antimicrobial activity of endophytic fungi associated with the alpine plant Saussurea involucrata. Biol. Pharm. Bull. 33 (8): 1300–1306.
35.
Naik B.S., Shashikala J., Krishnamurthy Y.L. 2009. Study on the diversity of endophytic communities from rice (Oryza sativa L.) and their antagonistic activities in vitro. Microbiol. Res. 164 (3): 290–296.
36.
Nithya K., Muthumary J. 2011. Bioactive metabolite produced by Phomopsis sp., an endophytic fungus in Allamanda cathartica linn. Recent Res. Sci. Technol. 3 (3): 44–48.
37.
Ogundare A.O., Adetuyi F.C., Akinyosoye F.A. 2006. Antimicrobial activities of Vernonia tenoreana. Afr. J. Biotechnol. 5 (18): 1663–1668.
38.
Orole O.O., Adejumo T.O. 2009. Activity of fungal endophytes against four maize wilt pathogens. Afr. J. Microbiol. Res. 3 (12): 969–973.
39.
Owen N.L., Hundley N. 2004. Biodiversity of Marine derived fungi and identification of their metabolites. Sci. Prog. 87: 79–99.
40.
Pandey B., Ghimire P., Agrawal V.P. 2004. Studies on the antibacterial activity of the actinomycetes isolated from the Khumbu region of Nepal. J. Biol. Sci. 23: 44–53.
41.
Petrini O. 1991. Fungal endophytes of tree leaves. p. 179–197. In: “Microbial Ecology of Leaves” (J.H. Andrews, S.S. Hirano, eds.). Springer-Verlag, New York, USA, 516 pp.
42.
Pitt J.I., Hocking A.D. 1985. Fungi and Food Spoliage. Academic Press, Springer, 519 pp.
43.
Puri S.C., Verma V., Amna T., Qazi G.N., Spiteller M. 2005. An endophytic fungus from Nothapodytes foetida that produces camptothecin. J. Nat. Prod. 68 (12): 1717–1719.
44.
Rabah F.L., Saker Elshafei A., Cheikh M.B., Hocine H. 2007. Screening, Isolation and characterization of a novel antimicrobial producing actinomycete, Strain RAF10. Biotechnology 6 (4): 489–496.
45.
Rajagopal K., Maheswari S., Kathiravan G. 2012. Diversity of endophytic fungi in some tropical medicinal plants – a report. Afr. J. Microbiol. Res. 6 (12): 2822–2827.
46.
Sampson K. 1935. Further observations on the systemic infection of Lolium. Trans. Brit. Mycol. Soc. 21: 84–97.
47.
Scholz E., Rimpler H. 1989. Proanthocyanidins from Krameria triandra root. Planta Med. 55 (4): 379–384.
48.
Sieber T., Riesen T.K., Müller E., Fried P.M. 1988. Endophytic fungi in four winter wheat cultivars (Triticum aestivum L.) differing in resistance against Stagonospora nodorum (Berk.) Cast. & Germ. = Septoria nodorum (Berk.) Berk. J. Phytopathol. 122 (4): 289–306.
49.
Silva C.F., Azevedo R.S., Braga C., Sliva R., Dias E.S., Schwan R.F. 2009. Microbial diversity in a bagasse-based compost prepared for the production of Agaricus brasiliensis. Braz. J. Microbiol. 40 (3): 590–600.
50.
Srividya S., Adarshana T., Deepika V.B., Kajingailu G., Nilanjan D. 2012. Streptomyces sp. 9p as effective biocontrol against chilli soilborne fungal phytopathogens. Eur. J. Exp. Biol. 2 (1): 163–173.
51.
Staniek A., Woerdenbag H.J., Kayser O. 2008. Endophytes: exploiting biodiversity for the improvement of natural product-based drug discovery. J. Plant Interact. 3 (2): 75–93.
52.
Stierle A., Strobel G.A., Stierle D. 1993. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 260 (5105): 214–216.
53.
Strobel G.A. 2003. Endophytes as sources of bioactive products. Microbes Infect 5 (6): 535–544.
54.
Strobel G., Daisy B. 2003. Bioprospecting for microbial endophytes and their natural products microbiol. Microbiol. Mol. Biol. Rev. 67 (4): 491–502.
55.
Strobel G., Daisy B., Castillo U., Harper J. 2004. Natural products from endophytic microorganisms. J. Nat. Prod. 67 (2): 257–268.
56.
Tejesvi M.V., Kini K.R., Prakash H.S., Subbiad V., Shetty H.S. 2007. Genetic diversity and antifungal activity of species pestalotiopsis isolated as endophytes from medicinal plants. Fungal Divers. 24: 37–54.
57.
Tian X.L., Cao L.X., Tan H.M., Zeng Q.G., Jia Y.Y., Han W.Q., Zhou S.N. 2004. Study on the communities of endophytic fungi and endophytic actinomycetes from rice and their antipathogenic activities in vitro. World J. Microbiol. Biotechnol. 20: 303–309.
58.
Verma V.C., Gond S.K., Kumar A., Mishra A., Kharwar R.N., Gange A.C. 2009. Endophytic actinomycetes from Azadirachta indica A. Juss.: isolation, diversity and anti-microbial activity. Microbiol. Ecol. 57 (4): 749–756.
59.
Wang F.W., Jiao R.H., Cheng A.B., Tan S.H., Song Y.C. 2007. Antimicrobial potentials of endophytic fungi residing in Quercus variabilis and brefeldin A obtained from Cladosporium sp. World J. Microbiol. Biotechnol. 23: 79–83.
60.
Xu L., Zhou L., Zhao J., Jiang W. 2008. Recent studies on the antimicrobial compounds produced by plant endophytic fungi. Nat. Product Res. Development 20: 731–740.
61.
Yadav J., Shrestha U.T., Tiwari K.B., Sahukhal G.S ., Agrawal V.P. 2008. Streptomycin – like antibiotic from Streptomyces spp. isolated from Mount Everest base camp. Nepal J. Sci. Technol. 9: 73–77.
62.
Yuan Z.L., Zhang C.L., Lin F.C., Kubicek C.P. 2010. Identity, diversity, and molecular phylogeny of the endophytic mycobiota in the roots of rare wild rice (Oryza granulate) from a nature reserve in Yunnan, (China). Appl. Environ. Microbiol. 76 (5): 1642–1652.
63.
Zhang H.W., Song Y.C., Tan RX. 2006. Biology and chemistry of endophytes. Nat. Product Rep. 23 (5): 753–771.
64.
Zin N.M., Sarmin N.I., Ghadin N., Basri D.F., Sidik N.M., Hess W.M., Strobel G.A. 2007. Bioactive endophytic streptomycetes from the Malay Peninsula. FEMS Microbiol. Lett. 274 (1): 83–88.
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
