The investigation was conducted in 2004 and 2005 to test 28 sesame genotypes for resistance and susceptablity to Rhizoctonia solani under artificial infection conditions at the Plant Breeding Experimental Farm of the Faculty of Agriculture, Suez Canal University, Ismailia, Egypt. All screened sesame genotypes showed varied significant degrees of infestation with the root rot pathogen. It is worth to mention that some of sesame genotypes kept their resistance characterestic classes as moderately resistant (MR) or resistant (R) during the two successive seasons. Such genotypes might be useful for breeding programs due to stability of their resistant character as well as their seed yield. Phenotypic coefficients of variation (P.C.V.) and genotypic coefficients of variation (G.C.V.) were of high value regarding resistance characters during both seasons and comparable to seed yield character. The heritability estimates indicate that selection is a suitable way for picking up sesame genotypes that have high chance for resistance character to root rot disease (R. solani) with high seed yield potential. The genetic advance and heritability estimates in all cases supported the selection of some sesame genotypes to be used in next breeding programs for root rot resistance, they also showed a high seed yield potential.