ORIGINAL ARTICLE
Plant extract effect on seed-borne pathogenic fungi from seeds of paddy grown in Southern India
1
Agricultural Microbiology Laboratory, Department of Microbiology and Biotechnology, Bangalore University
Jnana Bharathi Campus, Bangalore-560 056, India
2
Agricultural Microbiology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore, India
Corresponding author
Devihalli Chikkaiah Mohana
Agricultural Microbiology Laboratory, Department of Microbiology and Biotechnology, Bangalore University Jnana Bharathi Campus, Bangalore-560 056, India
Agricultural Microbiology Laboratory, Department of Microbiology and Biotechnology, Bangalore University Jnana Bharathi Campus, Bangalore-560 056, India
Journal of Plant Protection Research 2011;51(2):101-106
KEYWORDS
TOPICS
ABSTRACT
A total of 40 seed samples of different cultivars of paddy, collected from southern part of India, were mycologicaly analyzed
by agar plating method on Czapek’s-Dox-Agar (CzA) medium and Standard Blotter Method (SBM), revealed the occurrence of 33 di-
verse fungal species belonging to 16 genera. The species of
Drechslera oryzae
(RP 82.5%) was recorded with the incidence and relative
preponderance (RP), followed by
Curvularia lunata
(RP 67.5%) and
Aspergillus niger
(RP 65.0%) respectively. The data on the diversity
and incidence of fungal species would be a great importance in the region for predicting the extent of pre-and post-infections.
In vitro
antifungal activity assay of methanol extract of 12 plants belonging to different families were tested against eight pathogenic fungal species viz.,
Alternaria alternata
,
Aspergillus flavus, Curvularia lunata, Drechslera oryzae
,
D. halodes
,
Fusarium moniliforme
,
Pyricularia oryzae
and
Trichoconis padwickii
by poisoned food technique. The results revealed that, methanol extract of
Acacia nilotica,
Caesalpinia coriaria
,
Decalepis hamiltonii
,
Emblica officinalis, Lawsonia inermis
and
Mimosops elengi
showed significant antifungal activity
at 3500 μg/ml concentration. Comparative evaluation of the extracts with the synthetic fungicides viz., bavistin, blitox, captan, dithane
M-45 and thiram at the recommended dosage revealed that, antifungal activity of methanol extract of
D. hamiltonii, L. inermis
and
M. elengi
was almost equivalent.
These plants can possibly be exploited in the management of seed-borne pathogenic fungi and prevention of biodeterioration of paddy in an eco-friendly way
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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