ORIGINAL ARTICLE
Pathogenicity of endogenous isolate of Paramyrothecium (=Myrothecium) roridum (Tode) L. Lombard & Crous against the squash beetle Epilachna chrysomelina (F.)
1
Department of Plant Protection, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region, Duhok, Iraq
2
Duhok Research Center, College of Veterinary Medicine, Duhok University, Kurdistan Region, Duhok, Iraq
3
Plant Protection, General Directorate of Agriculture-Duhok, Kurdistan Region, Duhok, Iraq
4
Department of Medical Laboratory Techniques, Al-Noor University College, Nineva, Iraq
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2020-09-14
Acceptance date: 2020-12-08
Online publication date: 2021-03-01
Corresponding author
Feyroz Ramadan Hassan
Department of Plant Protection, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region – Iraq, Zakho, 42, Duhok, Iraq
Department of Plant Protection, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region – Iraq, Zakho, 42, Duhok, Iraq
Journal of Plant Protection Research 2021;61(1):110-116
KEYWORDS
TOPICS
ABSTRACT
The squash beetle Epilachna chrysomelina (F.) is an important insect pest which causes severe
damage to cucurbit plants in Iraq. The aims of this study were to isolate and characterize
an endogenous isolate of Myrothecium-like species from cucurbit plants and from soil
in order to evaluate its pathogenicity to squash beetle. Paramyrothecium roridum (Tode) L.
Lombard & Crous was isolated, its phenotypic characteristics were identified and ITS
rDNA sequence analysis was done. The pathogenicity of P. roridum strain (MT019839) was
evaluated at a concentration of 107 conidia · ml–1) water against larvae and adults of E. chrysomelina
under laboratory conditions. The results revealed the pathogenicity of the isolate
to larvae with variations between larvae instar responses. The highest mortality percentage
was reported when the adults were placed in treated litter and it differed significantly from
adults treated directly with the pathogen. Our results documented for the first time that
P. roridum has potential as an insect pathogen.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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