ORIGINAL ARTICLE
Occurrence of Wheat dwarf virus and Barley yellow dwarf virus species in Poland in the spring of 2019
1
Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2020-07-01
Acceptance date: 2020-07-02
Online publication date: 2020-10-07
Corresponding author
Katarzyna Trzmiel
Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul. Węgorka 20, 60-318, Poznań, Poland
Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul. Węgorka 20, 60-318, Poznań, Poland
Journal of Plant Protection Research 2020;60(4):345-350
KEYWORDS
Barley yellow dwarf virusdouble antibody sandwich enzyme-linked immunosorbentassay (DAS-ELISA)Duplex-immunocapture-polymerase chain reaction(Duplex-IC-PCR) reverse transcription-polymerase chain reaction connected with restrictionfragment length polymorphism analyses (RT-PCR-RFLP)Wheat dwarf virus
TOPICS
ABSTRACT
In the spring of 2019, many plants, mainly winter wheat, were observed to have dwarfism
and leaf yellowing symptoms. These plants from several regions of Poland were collected
and sent to the Plant Disease Clinic of the Institute of Plant Protection – National
Research Institute in Poznań to test for the presence of viral diseases. Double antibody
sandwich enzyme-linked immunosorbent assay (DAS-ELISA) results showed numerous
cases of Wheat dwarf virus (WDV) and a few cases of plant infections caused by Barley
yellow dwarf viruses (BYDVs). WDV was detected in 163 out of 236 tested winter wheat
plants (69.1%), in 10 out of 27 tested winter barley plants (37%) and in 6 out of 7 triticale
plants (85.7%) while BYDVs were found, respectively, in 9.7% (23 out of 236) and in 18.5%
(5 out of 27) of tested winter forms of wheat and barley plants. Infected plants came mainly
from the regions of Lower Silesia and Greater Poland. Furthermore, individual cases of
infections were also confirmed in the following districts: Lubusz, Opole, Silesia, Kuyavia-
-Pomerania and Warmia-Masuria. Results of Duplex-immunocapture-polymerase chain reaction
(Duplex-IC-PCR) indicated the dominance of WDV-W form in wheat and WDV-B
form in barley plants. Moreover, results of reverse transcription – polymerase chain reaction
(RT-PCR) connected with restriction fragment length polymorphism (RFLP) analysis,
performed for 17 BYDVs samples, revealed 8 BYDV-PAS, 4 BYDV-MAV and 2 BYDVPAV
as well as the presence of two mixed infections of BYDV-MAV/-PAS and one case of
BYDV-MAV/-PAV. Next, RT-PCR reactions confirmed single BYDV-GAV infection and
the common presence of BYDV-SGV. To the best of our knowledge, in 2020 the viruses
were not a big threat to cereal crops in Poland.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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