ORIGINAL ARTICLE
Occurrence and molecular characterization of a 16SrI-R subgroup phytoplasma associated with Aquilegia vulgaris phyllody disease
1
Plant Protection Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, AREEO, Shahrekord, Iran
2
Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Center, AREEO, Yazd, Iran
3
Department of Agricultural and Food Sciences, Alma Mater Studiorum University of Bologna, Viale Fanin, Bologna, Italy
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2020-12-14
Acceptance date: 2021-03-02
Online publication date: 2021-04-23
Corresponding author
Ghobad Babaei
Plant Protection Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, AREEO, Shahrekord, Iran
Plant Protection Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Center, AREEO, Shahrekord, Iran
Journal of Plant Protection Research 2021;61(3):222-228
KEYWORDS
TOPICS
ABSTRACT
During 2016–2017 surveys, carried out for phytoplasma diseases in ornamental plants in
Chaharmahal and Bakhtiari provinces, Iran, found symptoms of virescence, phyllody, reduced
size of leaves and flowers in columbine (Aquilegia vulgaris). Total DNAs extracted
from symptomatic and symptomless plants were tested for the presence of phytoplasma
using P1/P7 and R16F2n/R16R2 primers in direct and nested PCR producing amplicons
of about 1.8 and 1.2 kb, respectively, from all symptomatic A. vulgaris plants, but not from
symptomless ones. The consensus sequence of the detected phytoplasma named Aquilegia
phyllody (APh) was 100% identical with strains clustering to phytoplasmas enclosed in the
16SrI group as also confirmed by phylogenetic analyses. Both real and virtual restriction
fragment length polymorphism analysis of R16F2n/R16R2 amplicons showed profiles that
were identical to each other and indicated the affiliation of the APh phytoplasma to the
16SrI-R subgroup. This is the first report of a 16SrI-R phytoplasma associated with this
A. vulgaris phyllody disease.
ACKNOWLEDGEMENTS
We would like to thank Farshid Shabani at the Division
of Plant Protection, CHB Agricultural Organization for
supporting this work.
FUNDING
This research was supported by
Chaharmahal and Bakhtiari Agricultural and Natural
Resources Research and Education Center, AREEO,
Shahrekord, Iran.
RESPONSIBLE EDITOR
Natasza Borodynko-Filas
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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