ORIGINAL ARTICLE
Nitrate non-utilizing mutants and vegetative compatibility groups of Fusarium proliferatum and F. sacchari isolated from rice in the peninsular Malaysia and Kalimantan, Indonesia
1
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
2
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia
3
Malaysian Agricultural Research and Development Institute, Seberang Perai Research Station, 13200 Kepala Batas, Pulau Pinang, Malaysia
4
Department of Agrotechnology, Faculty of Agrotechnology and Food Sciences, Universiti Malaysia Terengganu
21030 Kuala Terengganu, Terengganu, Malaysia
Corresponding author
Nur Ain Izzati Mohd Zainudin
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Journal of Plant Protection Research 2009;49(2):229-235
KEYWORDS
TOPICS
ABSTRACT
A total of 26 isolates of Fusarium proliferatum and F. sacchari were isolated from rice in the Peninsular Malaysia and Kalimantan, Indonesia. Spontaneous chlorate-resistant sectors (CRSs) were recovered from all wild type of both Fusarium species when cultured on two chlorate media. The non-utilizing (nit) mutants were generated as crn (chlorate resistant, nitrate utilizing), nit1, nit3 and nitM based on phenotyping growth-types on diagnostic media with different sources of nitrogen. The nit mutants were paired on minimal medium (MM) for examining the vegetative compatibility. The majority of nit mutants (32.3–46.5%) recovered were nit1.Eight and seven vegetative compatibility groups (VCGs) of F. proliferatum and F. sacchari were identified, respectively. The isolates of F. proliferatum and F. sacchari were genetically diverse as shown by the number and distribution of the VCGs. No strong correlation was observed between VCGs of both species and location
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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