ORIGINAL ARTICLE
New fungi causing postharvest spoilage of cucumber fruits and their molecular characterization in Egypt
1
Plant Pathology Department, National Research Centre, Cairo, Egypt
2
Genetics and Cytology Department, National Research Centre, Cairo, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2017-11-15
Acceptance date: 2018-11-26
Online publication date: 2018-12-17
Corresponding author
El Sayed Hussein Ziedan
Plant Pathology Department, National Research Centre, Tahrir Street, 12622 Cairo, Egypt
Plant Pathology Department, National Research Centre, Tahrir Street, 12622 Cairo, Egypt
Journal of Plant Protection Research 2018;58(4):362-371
KEYWORDS
TOPICS
ABSTRACT
This work was carried out during two successive seasons (2016 and 2017) on cucumber
fruits from a plastic greenhouse and from open field cultivation in El Gharbeia and El Giza
Governorates, Egypt. Isolation trials from spoilage fruit samples of plastic greenhouse cultivation
recorded high frequency of Alternaria tenusinium, Fusarium spp. and Pleospora alli.
The most common fungi of rotten cucumber fruits from an open field were Galactomyces
spp. and Fusarium spp. Pathogenicity tests proved that, Fusarium solani from El-Gharbeia
followed by A. tenusinium from El-Giza were the most frequent isolates responsible for
rot of cucumber fruits from plastic greenhouse cultivation. Moreover, the most frequent
isolates causing postharvest disease of cucumber fruits of the open field were Galactomyces
candidium from El-Giza followed by Geotrichum sp. and F. fujikuroi from El-Gharbeia
Governorates, respectively. This is the first report of several fungi causing postharvest fruit
rot disease of cucumber i.e., G. candidium, Geotrichum sp., A. tenusinium, P. alli and Fusarium
spp. (F. fujikuroi, F. verticiolides, F. solani, F. geraminearium and Fusarium incarnatum).
Fungal isolates were identified according to cultural, morphological and molecular
characterization based on sequencing of internal transcribed spacer1 (ITS1). All the ITS
nucleotide sequences of fungi were applied and conserved in GenBank.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (36)
1.
Agwanande A.W., Leopold T.N., Michel J.D.P., Priya P., Mariya A., Manilal V.B., Krishnakumar B., Henri A.Z.P. 2016. Isolation and molecular identification of fungi in stored maize (Zea mays L.) and groundnuts (Arachis hypogaea L.) in Ngaoundere, Cameroon. American Journal of Microbiological Research 4 (3): 85−89. DOI: 10.12691/ajmr-4-3-4.
2.
Alam M.W., Rehman A., Malik A.U., Iqbal Z., Amin M., Ali S., Hameed A., Sarfraz S. 2017. First report of Geotrichum candidum causing postharvest sour rot of peach in Punjab. Pakistan Plant Disease 101 (8): 1543.
3.
Alice L.A., Campbell C.S. 1999. Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. American Journal of Botany 86 (1): 81–97. DOI: 10.2307/2656957.
4.
Al-Sadi A.M., Al-Said F.A., Al-Kaabi S.M., Mohammed S., Al-quraini S.M., Al-Mazroui S.S., Al-Mahmooli I.H., Deadman L.D. 2011. Occurrence, characterization and management of fruit rot of immature cucumbers under greenhouse conditions in Oman. Phytopathologia Mediterranea 50 (3): 421−429.
5.
Alwakeel S.S. 2013. Molecular identification of isolated fungi from stored apples in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences 20 (4): 311−317. DOI: https://doi.org/10.1016/j.sjbs....
6.
An R.P., Ma Q. 2005−2006. Control of cucumber grey mold by endophytic bacteria. Cucurbit Genetics Cooperative Report 28−29: 1−6.
7.
Baldwin B.G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae. Molecular Phylogenetics and Evolution 1 (1): 3–16. DOI: https://doi.org/10.1016/1055-7....
8.
Barnett H.L., Hunter B.B. 1998. Illustrated Genera of Imperfect Fungi. 4th ed. APS Press, St. Paul, Minnesota, USA, 218 pp.
9.
Barry C.S., Llop-Tous M.I., Grierson D. 2000. The regulation of 1-aminocyclopropane-1-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiology 123 (3): 979−986. DOI: https://doi.org/10.1104/pp.123....
10.
Blancard D., Lecoq H., Pitrat M. 2005. A color atlas of cucurbit diseases observation, identification and control Manson Publishing Ltd. London, United Kingdom, 304 pp.
11.
Boekhout T., Kurtzman C.P., O’Donnell K., Smith M.T. 1994. Phylogeny of the yeast genera Hanseniaspora (anamorph Kloeckera), Dekkera (anamorph Brettanomyces), and Eeniella as inferred from partial 26s ribosomal DNA nucleotide sequences. International Journal of Systematic Bacteriology 44 (4): 781–786. DOI: 10.1099/00207713-44-4-781.
12.
Booth C. 1971. The Genus Fusarium. Commonwealth Mycological Institute, Kew Surrey, England, 237 pp.
13.
Cai J., Roberts I.N., Collins, M.D. 1996. Phylogenetic relationships among members of the ascomycetous yeasts genera Brettanomyces, Debaryomyces, Dekkera and Kluyveromyces deduced by small subunit rRNA gene sequences. International Journal of Systematic Bacteriology 46 (2): 542−549.
14.
Cohen Y., Crisi U., Mosinger E. 1991. Systemic resistance of potato plants against Phytophthora infestans induced by unsaturated fatty acids. Physiological and Molecular Plant Pathology 38 (4): 255−268. DOI: https://doi.org/10.1016/S0885-....
15.
Di Francesco A., Fruk M., Martini C., Jemric T., Mari M. 2015. First report of Asiatic brown rot (Monilinia polystroma) on apple in Croatia. Plant Disease 99 (8): 1181. DOI: https://doi.org/10.1094/PDIS-1....
16.
Effiuvwevwere B.J.O. 2000. Microbial Spoilage Agents of Tropical and Assorted fruits and Vegetables (An Illustrated References Book). Paragraphics Publishing Company, Port Harcourt, Nigeria, 39 pp.
17.
Ellis M.B. 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, England, 608 pp.
18.
Farrag E.S.H., Ziedan E.H., Mahmoud S.Y.M. 2007. Systemic acquired resistance induced in cucumber plants against powdery mildew disease by pre-inoculation with tobacco necrosis virus. Plant Pathology Journal 6 (1): 44−50. DOI: 10.3923/ppj.2007.44.50.
19.
Hall L., Wohlfiel S., Roberts G.D. 2003. Experience with the MicroSeq D2 large-subunit ribosomal DNA sequencing kit for identification of filamentous fungi encountered in the clinical laboratory. Journal of Clinical Microbiology 42 (2): 622–626. DOI: 10.1128/JCM.42.2.622-626.2004.
20.
James S.A., Collins M.D., Roberts I.N. 1996. Use of an rRNA internal transcribed spacer region to distinguish phylogenetically closely related species of the genera Zygosaccharomyces and Torulaspora. International Journal of Systematic Bacteriology 46 (1): 189−194. DOI: 10.1099/00207713-46-1-189.
21.
Jeewon R., Ittoo J., Mahadeb D., Jaufeerally-Fakim Y., Wang H.K., Liu A R. 2013. DNA Based identification and phylogenetic characterisation of endophytic and saprobic fungi from Antidesma madagascariense, a medicinal plant in Mauritius. Journal of Mycology 2013: 1−10. DOI: http://dx.doi.org/10.1155/2013....
22.
Jukes T.H., Cantor C.R. 1969. Evolution of protein molecules. p. 21−132. In: “Mammalian Protein Metabolism” (H.N. Munro, ed.). Academic Press, New York.
23.
Khot P.D., Ko D.L., Fredricks D.N. 2009. Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays. Applied and Environmental Microbiology 75 (6): 1559–1565. DOI: 10.1128/AEM.02383-08.
24.
Kim Y.K., Kim T.S., Shim H.S., Park K.S., Yeh W.H., Hong, S.J., Shim C.K., Kim J.S., Park J.H., Han E.J., Lee M.H., Jee H.J. 2011. First report of sour rot on post-harvest oriental melon, tomato, cucumber, potato, pumpkin and carrot caused by Geotrichum candidum. Results Plant Disease 17 (2): 232−234. DOI: 10.5423/RPD.2011.17.2.232.
25.
Kurtzman C.P. 1992. rRNA sequence comparisons for assessing phylogenetic relationships among yeasts. International Journal of Systematic Bacteriology 42: 1−6. DOI: 10.1099/00207713-42-1-1.
26.
Li W.H. 1997. Molecular Evolution. Sinauer Associates, Inc., Publishers Sunderland, MA, 479 pp.
27.
Maiko W. 2013. Molecular phylogeny and identification of Fusarium species based on nucleotide sequences. Mycotoxins 63 (2): 133−142. DOI: https://doi.org/10.2520/myco.6....
28.
Nelson P.E., Toussoum T.A., Marasas W.F.O. 1983. Fusarium Species. An Illustrated Manual for Identification. The Pennsylvania University, USA, 206 pp.
29.
Saitou N., Nei M. 1987. The neighbor joining method: a new method for constructing phylogenetic trees. Molecular Biology and Evolution 4 (4): 406−425. DOI: 10.1093/oxfordjournals.molbev.a040454.
30.
Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning; A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, 1659 pp. DOI: https://doi.org/10.1016/0167-7....
31.
Sang T., Crawford D.J., Stuessy T.F. 1995. Documentation of reticulate evolution in Peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. Proceedings of the National Academy of Sciences of the United States of America 92: 6813–6817.
32.
Sani M.A., Usman N., Kabir F., Kutama A.S. 2015. The effect of three natural preservatives on the growth of some predominant fungi associated with the spoilage of fruits (Mango, Pineapple and Cucumber) Global Advanced Results Journal of Agricultural Sciences 4 (12): 923−928.
33.
Snedecor G.W., Cochran W.G. 1980. Statistical Methods. 7th ed. Iowa State University Press, Ames, 705 pp.
34.
Soliman H.M., El Metwall M.A., Elkahky M.T., Badawi W. 2015. Alternative to chemical control of grey mold disease on cucumber caused by Botrytis cinerea Pers. Asian Journal of Plant Pathology 9 (1): 1−15. DOI: 10.3923/ajppaj.2015.1.15.
35.
Suwannarach N., Kumla J., Nitiyon Limtong S.S., Lumyong S. 2016. First report of sour rot on tomato caused by Galactomyces reessii in Thailand. Journal of General Plant Pathology 82 (4): 228–231. DOI: 10.1007/s10327-016-0663-x.
36.
Ziedan E.H.E., Saad M.M. 2016. Efficacy of nanoparticles on seed borne fungi and their pathological potential of cucumber. International Journal of PharmTech Research 9 (10): 16−24.
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
