ORIGINAL ARTICLE
Nematicidal activity of some biopesticide agents and microorganisms against root-knot nematode on tomato plants under greenhouse conditions
1
Central Agricultural Pesticides Laboratory, Agriculture Research Center, Integrated Protection Laboratory, El-Sabaheya,
Baquse, 21616, Alexandria, Egypt
2
Faculty of Agriculture, Saba Basha – Alexandria University, 22 Taj El-Roasaa, Saba Basha, Bulkeley, 21531, Alexandria, Egypt
3
Plant Protection Research Institute, Agriculture Research Center, Plant Protection Research Station,
El-Sabaheya, Baquse, 21616, Alexandria, Egypt
Corresponding author
Mohamed Salah El-din Hassan Khalil
Central Agricultural Pesticides Laboratory, Agriculture Research Center, Integrated Protection Laboratory, El-Sabaheya, Baquse, 21616, Alexandria, Egypt
Central Agricultural Pesticides Laboratory, Agriculture Research Center, Integrated Protection Laboratory, El-Sabaheya, Baquse, 21616, Alexandria, Egypt
Journal of Plant Protection Research 2012;52(1):47-52
KEYWORDS
TOPICS
ABSTRACT
A pot experiment was carried out under greenhouse conditions to study the impact of the evaluated treatments namely
abamectin, azadirachtin 0.15%,
azadirachtin 0.03%,
Bacillus subtilis
,
Pseudomonas fluorescens, Paecilomyces lilacinus
and oxamyl against
root-knot nematode (
Meloidogyne
incognita
) on the tomato plants cv. Super strain B. The results indicated that the most of the tested
treatments obviously reduced root galls and remarkably increase tomato plant growth characters significantly and egg masses on root
system, as well as, juvenile’s numbers in the soil.
P. lilacinus
was the most effective treatment on both galls and egg masses achieving 88.23 and 76.94% reduction, respectively. While,
less effective treatment was
P. fluorescens
achieving 57.53% galls reduction. Azadirachtin 0.03% was the least effective treatment giving
40.37% reduction of egg masses.
The superior treatment that suppressed nematode populations was oxamyl recording (88.90%) followed by abamectin (78.69%) re
-
duction. Moreover, azadirachtin 0.15% was the least effective treatment which recorded 60.15% reduction.
On the other hand, plants free nematode recorded the highest plant parameters for shoot system length, fresh shoot weight, dry shoot
weight and root system length with values of 24.15, 107.53, 211.59 and 46.17% increase, respectively. Azadirachtin 0.15% was the least
effective treatment on shoot system length and fresh and dry shoot weight. While, oxamyl recorded the least increase in root system
length estimated by 18.47%.
B. subtilis
recorded the highest increase in fresh root weight followed by
P. fluorescens
with value of 125.75 and 86.57%, consecutively. Vise
versa,
P. fluorescens
was the superior treatment to increase the dry root weight by 68.14% followed by
B. subtilis
which recorded 35.40%.
The least effective treatment in improving fresh root weight was azadirachtin 0.15% which recorded 54.85% increase. Regarding to dry
shoot weight
P. lilacinus
and azadirachtin 0.15% were the least effective treatments with values of 8.85 and 2.66% reduction, respectively
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (35)
1.
Alam M.M., Ahmad M., Khan A.M. 1980. Effect of organic amendment on the growth and chemical composition of tomato,eggplants and chilli and their susceptibility to attack by Meloidogyne incognita. Plant Soil 57: 231–236.
2.
Basu M.J., Karuppagnaniar S. 2009. Impact of Glomus fasiculatum and fluorescent pseudomonas on growth performance of Figna radiata (L.) Wilczek challenged with phytopathogens. J. Plant Protection Res. 49 (2): 190–194.
3.
Bonants P.J.M., Fitters P.F.L., Den Belder E., Waal- wijik C., Henfling J.W.D.M. 1995. A basic serine protease from Paecilo myces lilacinus with biological activity against Meloidogyne hapla eggs. Microbiology 141: 775–784.
4.
Cochran A., Watrin C., Ulmer B. 2007. Review of nematode protection benefits from abamectin seed treatment on corn. J. Nematol. 39, p. 78. (Abstract).
5.
Coleman D.C., Crossley D.A. 1996. Fundamentals of Soil Ecology. Academic Press, San Diego, CA, 205 pp.
6.
Corbett J.R., Wright K., Baillie A.C. 1984. The Biochemical Mode of Action of Pesticides. 2nd ed London, Academic Press, 382 pp.
7.
Costat Software. 1988. Microcomputer Program Analysis. CoHort software, Berkely, CA, USA.
8.
Devakumar C., Goswami B.K., Mukherjee S.K. 1985. Nematicidal principles from neem (Azadirachta indica) part 1,screening of neem kernel fraction against Meloidogyne incognita. Indian J. Nematol. 15: 121–124.
10.
Faske T.R., Starr J.L. 2006. Sensitivity of Meloidogyne incognito and Rotylenchulus reniformis to abamectin. J. Nematol. 38 (2): 240–244.
11.
Faske T.R, Starr J.L. 2007. Cotton root protection from plant parasitic nematodes by abamectin treated seed. J. Nematol. 39 (1): 27–30.
12.
Holbrook C.C., Knauft D.A., Dikson D.W. 1983. A technique for screening peanut for resistance to Meloidogyne arenaria. Plant Dis. 57: 957–958.
14.
Javed N., Gowen S.R., El-hassan S.A., Inam-ul-Haq M., Shahina F., Pembroke B. 2008. Efficacy of neem (Azadirachta indica) formulations on biology of root-knot nematodes (Meloidogyne javanica) on tomato. Crop Protect. 27: 36–43.
15.
Jatala P., Franco J., Gonzales A., O’Hara C.M. 1985. Hatching stimulation and inhibition of Globodera pallida eggs by enzymatic and exopathic toxic compounds of some biocontrol fungi. J. Nematol. 17, p. 501.
16.
Jatala P. 1986. Biological control of plant parasitic nematodes. Ann. Rev. Phytopathol. 24: 453–489.
17.
Kavitha J., Jonathan E.I., Umamaheswari R. 2007. Field application of Pseudomonas fluorescens, Bacillus subtilis and Trichoderma viride for the control of Meloidogyne incognita(Kofoid and White) Chitwood on sugarbeet. J. Biol. Control Soc. Biocontrol Adv., Bangalore, India. 21 (2): 211–215.
18.
Khan A.M., Alam M.M., Ahmad R. 1974. Mechanism of the control of plant-parasitic nematodes as a result of the application of oil-cakes to the soil. Indian J. Nematol. 4: 93–96.
19.
Khan A., Williams K.L., Nevalainen H.K.M. 2006. Control of plant-parasitic nematodes by Paecilomyce lilacinus and Monacrosporium lysipagum in pot trials. Biocontrol 51: 643–658.
20.
Korayem A.M., Mahmoud M.A.Y., Moawad M.M.M. 2008. Effect of chitin and abamectin on Meloidogine Incognito infesting rapeseed. J. Plant Protection Res. 48 (3): 365–370.
21.
Krishnaveni M., Subramanian S. 2004. Evaluation of biocontrol agents for the management of Meloidogyne incognito on cucumber (Cucumis sativus L). Current Nematol. 15 (1/2): 33–37.
22.
Linderman R.G. 1992. Vesicular-arbuscular mycorrhizae and soil microbial interactions. p. 45–70. In:“Mycorrhizae in Sustainable Agriculture” (G.J. Bethlenfalvay, R.G. Linderman, eds.). Amer. Soc. Agron. Special Publication 54. Amer. Soc. Agron., Madison, WI, 124 pp.
23.
Monfort W.S., Kirkpatrick T.L., Long D.L., Rideot T. 2006. Efficacy of a novel nematicidal seed treatment against Meloidogyne incognito on cotton. J. Nematol. 38 (2): 245–249.
24.
Oclarit E.L., Joseph C., Cumagun R. 2009. Evaluation of efficacy of Paecilomyces Lilacinus as biological control agent of Meloidogyne incognita attacking tomato. J. Plant Protection Res. 49 (4): 337–340.
25.
Prakob W., Kanthasab V., Supina V., Chaimeungchern N., Kidtayo T. 2007. Use of arbuscular mycorrhizal fungi, antagonistic fungus and rhizobacteria P. aeruginosa and B. subtillis in controlling tomato root-knot nematodes. J. Agric. 23: 403–406.
26.
Randhawa N., Sakhuja P.K., Singh I. 2001. Management of rootknot nematode Meloidogyne incognito in tomato with organic amendments. Plant Dis. Res. 16: 274–276.
27.
Sakhuja P.K., Jain R.K. 2001. Nematode diseases of vegetable crops and their management. In: “Diseases of Fruits and Vegetables and their Management” (T.S. Thind, ed.). Kalyani Pub., Ludhiana, India, 474 pp.Sasser J.N., Eisenback J.D., Carter C.C., Triantaphyllou A.C. 1983. The international Meloidogyne project its goals and accomplishments. Ann. Rev. Phytopathol. 21: 271–288.
28.
Shanthi A., Sivakumar M. 2005. Ecofriendly approaches for the management of root-knot nematode, Meloidogyne incognito in tomato. J. Plant Protect. Environ. 2 (2): 154–157.
29.
Sharma H.K., Kamra A., Panka J., Lal J., Kumar J. 2008. Effect of seed treatment with Pseudomonas fluorescens alone and in combination with soil application of carbofuran and neem seed powder against Meloidogyne incognito in okra. Pestic. Res., J. Soc. Pestic. Sci. India. 20 (1): 79–82.
30.
Sharon E., Bar-Eyal M., Chet I., Herrera-Estrella A., Kleifeld O., Spiegel Y. 2001. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology 91: 687–693.
31.
Siddiqui I.A., Ehetshamul-Haque S., Shahid-Shaukat S. 2001. Use of rhizobacteria in the control of root rot and root knot disease complex of mungbean. J. Phytopathol. 149: 337–346.
32.
Siddiqui Z.A., Mahmood I. 1999. Role of bacteria in the management of plant parasitic nematodes: A review. Bioresource Technol. 69: 167–179.
33.
Sikora R.A., Fernandez E. 2005. Nematode parasites of vegetables. p. 319–392. In: “Plant-Parasitic Nematodes in Subtropical and Tropical Agriculture” (M. Luck, R.A. Sikora, J. Bridge, eds.). 2nd ed. CABI Publishing, Wallingford, UK,.896 pp.
34.
Stirling G.R. 1991. Biological Control of Plant Parasitic Nematodes. CAB International, Wallingford, UK, 220 pp.
35.
Weller D.M. 1988. Biological control of soil borne plant pathogens in the rhizosphere with bacteria. Ann. Rev. Phytopatol. 26: 379–407.
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