ORIGINAL ARTICLE
Nanoactivities of natural nanomaterials rosmarinic acid, glycyrrhizic acid and glycyrrhizic acid ammonium salt against tomato phytopathogenic fungi Alternaria alternata and Penicillium digitatum
1
Post Harvest Department, Plant Pathology Institute, Agriculture Research Centre, Giza, Egypt
2
Environmental Biotechnology, Misr University for Science and Technology, College of Biotechnology, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2019-07-18
Acceptance date: 2019-10-14
Online publication date: 2020-05-26
Corresponding author
Tahsin Mansour Shoala
Environmental Biotechnology Department, College of Biotechnology, Misr University for Science and Technology, 6th of October City, Egypt
Environmental Biotechnology Department, College of Biotechnology, Misr University for Science and Technology, 6th of October City, Egypt
Journal of Plant Protection Research 2020;60(2):150-160
KEYWORDS
TOPICS
ABSTRACT
Black mold and green mold caused by Alternaria alternata and Penicillium digitatum, respectively,
are the most important decay pathogens of tomato fruits during storage. Our
research was aimed to control tomato phytopathogenic fungi A. alternata and P. digitatum
in vitro and in vivo by using natural nanomaterials rosmarinic acid (RA-NPs) at concentrations
of 0.3 and 0.6 mM, glycyrrhizic acid (GA-NPs) and glycyrrhizic acid ammounium
salt (GAS-NPs) (0.1–0.2 mM). Characterizations of the tested nanoparticles were carried
out by using dynamic light scattering which revealed that synthesized nanoparticles had
particle sizes of less than 100 nm. In vitro studies revealed that the three tested nanoparticles
reduced the growth of A. alternata and P. digitatum. Glycyrrhizic acid nanoparticles
were the most effective in reducing the growth of the two tested pathogens followed by
RA-NPs at 0.6 mM. Observations of A. alternata and P. digitatum by scanning electron
microscopy (SEM) showed severe damage in the hyphae and deformities in the conidia due
to the effect of the tested nanoparticles. In vivo results showed that, dipping tomato fruits
as a post-harvest treatment in all of the tested nanoparticles at different concentrations,
then stored at 10 ± 1°C and 90–95% relative humidity (RH) for 20 days greatly reduced the
disease severity of infected fruits with the two tested pathogens. GA-NPs at 0.2 mM significantly
reduced the development of black mold rot on tomato fruits. RA-NPs at 0.6 mM had
the best effect in controlling P. digitatum of all naturally and artificially inoculated tomato
fruits. Also, individual treatments of tomato fruits with RA-NPs, GA-NPs and GAS-NPs
significantly reduced postharvest losses of fruit since they delayed decay and maintained
fruit quality characteristics such as fruit firmness, titratable acidity and total soluble solids
during cold storage.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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