ORIGINAL ARTICLE
Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.)
1
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2020-06-30
Acceptance date: 2020-09-03
Online publication date: 2020-11-06
Corresponding author
Roksana Skowrońska
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Poland
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Poland
Journal of Plant Protection Research 2020;60(4):388-398
KEYWORDS
TOPICS
ABSTRACT
Currently, production of wheat cultivars (Triticum aestivum L.) that show durable field
resistance against fungal pathogens is a priority of many breeding programs. This type of
resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages.
Until now, seven genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr75, Lr77 and Lr78)
conferring durable types of resistance against multiple fungal pathogens have been identified
in the wheat gene pool. In this study we showed a multiplex Polymerase Chain Reaction
(multiplex PCR) assay, which was developed for detection of slow rusting resistance
genes Lr34, Lr46, Lr68, using molecular markers: csLV34, Xwmc44 and csGS, respectively.
Identification of molecular markers was performed on 40 selected wheat genotypes which
are the sources of slow rusting genes according to literature reports. Multiplex PCR is an
important tool to reduce the time and cost of analysis. This multiplex PCR protocol can be
applicable for genotyping processes and marker assisted resistance breeding of wheat.
ACKNOWLEDGEMENTS
The authors would like to acknowledge and thank Dr.
Harrold Bockelman at the USDA/ARS Small Grains
Laboratory, Aberdeen (ID, USA) for providing the
seeds of wheat accessions. In addition, we would like
to thank all of the reviewers and manuscript editor for
their careful review of the manuscript and for their excellent
suggestions for improving our initial work.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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