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Monitoring the occurrence of bacteria in stored cabbage heads
 
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1
Mendeleum – Institute of Genetics and Plant Breeding, Mendel University in Brno, Faculty of Horticulture, Valtická 337, 691 44 Lednice, Czech Republic
 
2
Department of Vegetable Growing and Floriculture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech Republic
 
3
Department of Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Krakow, Poland
 
 
Submission date: 2016-12-05
 
 
Acceptance date: 2017-01-23
 
 
Corresponding author
Dorota Tekielska
Department of Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Krakow, Poland
 
 
Journal of Plant Protection Research 2017;57(1):56-61
 
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ABSTRACT
Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three different parts of the cabbage heads. Isolations were carried out from stalks, inner and superficial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identified according to the sequences obtained. The most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were also identified. The results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. The nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (27)
1.
Afsharmanesh H., Ahmadzadeh M., Javan-Nikkhah M., Behboudi K. 2010. Characterization of the antagonistic activity of a new indigenous strain of Pseudomonas fluorescens isolated from onion rhizosphere. Journal of Plant Pathology 92 (1):187–194.
 
2.
Agrios G.N. 2006. Bacterial Soft Rots. 5th Edn. Academic Press, San Diego, USA.
 
3.
Arzanlou M., Mousavi S., Bakhshi M., Khakvar R., Bandehagh A. 2016. Inhibitory effects of antagonistic bacteria inhabiting the rhizosphere of the sugarbeet plants, on Cercospora beticola Sacc., the causal agent of Cercospora leaf spot disease on sugarbeet. Journal of Plant Protection Research 56 (1): 6–14.
 
4.
Bhat K.A., Bhat N.A., Mohiddin F.A., Mir S.A., Mir M.R. 2012. Management of postharvest Pectobacterium soft rot of cabbage (Brassica oleracea var capitata L.) by biocides and packing material. African Journal of Agricultural Research 7 (28): 4066–4074.
 
5.
Bhat K.A., Masood S.D., Bhat N.A., Bhat M.A., Razvi S.M., Mir M.R., Habib M. 2010. Current status of postharvest soft rot in vegetables. Asian Journal of Plant Sciences 9 (4): 200–208.
 
6.
Bretschneider K.E., Gonella M.P., Robeson D.J. 1989. A comparative light and electron microscopical study of compatible and incompatible interactions between Xanthomonas campestris pv. campestris and cabbage (Brassica oleracea). Physiological and Molecular Plant Pathology 34 (4): 285–297.
 
7.
Brocklehurst T., Lund B.M. 1981. Properties of pseudomonads causing spoilage of vegetables stored at low temperature. Journal of Applied Bacteriology 50 (2): 259–266.
 
8.
Czajkowski R., Pérombelon M.C.M., Van Veen J.A., Van der Wolf J.M. 2011. Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Plant Pathology 60 (6): 999–1013.
 
9.
Davidsson P.R., Kariola T., Niemi O., Palva E.T. 2013. Pathogenicity of and plant immunity to soft rot pectobacteria. Frontiers in Plant Science 4: 191.
 
10.
De Jonghe V., Coorevits A., Van Hoorde K., Messens W., Van Landschoot A., De Vos P., Heyndrickx M. 2011. Influence of storage conditions on the growth of Pseudomonas species in refrigerated raw milk. Applied and Environmental Microbiology 77 (2): 460–470.
 
11.
Eichmeier A., Baránek M., Pidra M. 2010. Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates. Plant Protection Science 46 (8): 145–148.
 
12.
Eichmeier A., Čechová J., Peňázová E. 2015. Genetic diversity of partial hrpF and Zur genes in populations of Xanthomonas campestris pv. campestris in Brassica oleracea convar. capitata in the Czech Republic. Acta Horticulturae 1105: 180–188.
 
13.
King A.D., Bolin H.R. 1989. Physiological and microbiological storage stability of minimally processed fruits and vegetables. Food Technology 43 (2): 132–139.
 
14.
Klindworth A., Pruesse E., Schweer T., Peplies J., Quast C., Horn M., Glöckner F.O. 2013. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Research. Available on: https://doi.org/10.1093/nar/gk... [Accessed: September 4, 2016].
 
15.
Liao C.H., Wells J.M. 1987. Association of pectolytic strains of Xanthomonas campestris with soft rots of fruits and vegetables at retail markets. Phytopathology 77: 418–422.
 
16.
Mauzey S.J., Koike S.T., Bull C.T. 2015. First report of bacterial blight of cabbage (Brassica oleracea var. capitata) caused by Pseudomonas cannabina pv. alisalensis in California. Journal of General Plant Pathology 81: 331–350.
 
17.
Nadarasah G., Stavrinides J. 2011. Insects as alternative hosts for phytopathogenic bacteria. FEMS Microbiology Reviews 35: 555–575.
 
18.
Nazerian E., Sijam K., Mior Ahmad Z.A., Vadamalai G. 2011. First report of cabbage soft rot caused by Pectobacterium carotovorum subsp. carotovorum in Malaysia. Plant Disease 95 (4): 491–491.
 
19.
Nicholson W.L., Munakata N., Horneck G., Melosh H.J., Setlow P. 2000. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and Molecular Biology Reviews 64 (3): 548–572.
 
20.
Perombelon M.C.M., Kelman A. 1980. Ecology of the soft rot erwinias. Annual Review of Phytopathology 18: 361–387.
 
21.
Roberts S.J., Hiltunen L.H., Hunter P.J., Brough J. 1999. Transmission from seed to seedling and secondary spread of Xanthomonas campestris pv. campestris in Brassica transplants: effects of dose and watering regime. European Journal of Plant Pathology 105: 879–889.
 
22.
Roohie R.K., Umesha S. 2012. Development of multiplex PCR for the specific detection of Xanthomonas campestris pv. campestris in cabbage and correlation with disease incidence. Journal of Plant Pathology and Microbiology 3: 1–9.
 
23.
Togashi J., Koushi U.E.D.A., Namai T. 2001. Overwintering of Erwinia carotovora subsp. carotovora in diseased tissues in soil and its role as inoculum for soft rot of Chinese cabbage (Brassica campestris, Pekinensis group). Journal of General Plant Pathology 67 (1): 45–50.
 
24.
Valero M., Fernandez P.S., Salmeron M.C. 2003. Influence of pH and temperature on growth of Bacillus cereus in vegetable substrates. International Journal of Food Microbiology 82 (1): 71–79.
 
25.
Warth A.D. 1978. Relationship between the heat resistance of spores and the optimum and maximum growth temperatures of Bacillus species. Journal of Bacteriology 134 (3): 699–705.
 
26.
Zhao Y., Li P., Huang K., Wang Y., Hu H., Sun Y. 2013. Control of postharvest soft rot caused by Erwinia carotovora of vegetables by a strain of Bacillus amyloliquefaciens and its potential modes of action. World Journal of Microbiology and Biotechnology 29 (3): 411–420.
 
27.
Zhao Y., Selvaraj J.N., Xing F., Zhou L., Wang Y., Song H., Liu Y. 2014. Antagonistic action of Bacillus subtilis strain SG6 on Fusarium graminearum. PLoS One 9 (3): e92486.
 
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