ORIGINAL ARTICLE
Molecular phylogeny and pathogenicity of Fusarium oxysporum f. sp. elaeidis isolates from oil palm plantations in Cameroon
1
Department of Plant Sciences, Faculty of Science, University of Bamenda, Bamenda, Northwest Region, Cameroon
2
Department of Plant Science, Faculty of Science, University of Buea, Buea, Southwest Region, Cameroon
3
Department of Crop Production Technology, College of Technology, University of Bamenda, Bamenda, Northwest Region, Cameroon
4
Department of Plant Biology, Faculty of Science, University of Yaounde 1, Yaounde, Center Region, Cameroon
5
Department of Plant Biology, Faculty of Science, University of Dschang, Dschang, West Region, Cameroon
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-11-18
Acceptance date: 2023-01-30
Online publication date: 2023-03-15
Corresponding author
Rosemary Tonjock Kinge
Department of Plant Sciences, Faculty of Science, University of Bamenda, Bamenda, Northwest Region, Cameroon
Department of Plant Sciences, Faculty of Science, University of Bamenda, Bamenda, Northwest Region, Cameroon
Journal of Plant Protection Research 2023;63(1):122-136
HIGHLIGHTS
- This study reveals Cameroonian isolate within FOE clade 1 exhibited the greatest variability grouping with isolates from Suriname, Brazil and Democratic Republic of Congo. The pathogenicity of FOE isolates from Cameroon and has demonstrated that FOE in Africa is more diverse than previously reported including a lineage not previously observed outside of Cameroon
KEYWORDS
TOPICS
ABSTRACT
In Cameroon, oil palm (Elaeis guineensis Jacq.) is of economic importance. However, it
is affected by vascular wilt presumed to be caused by Fusarium oxysporum f. sp. elaeidis
(FOE). Accurate species identification requires molecular-based comparisons. The aim of
this work was to molecularly identify Fusarium species associated with diseased oil palms
and to determine the pathogenicity of selected isolates. Fungal samples of diseased palms
were collected from the canopies and the soil of five oil palm estates of the Cameroon
Development Corporation and characterized by sequencing and comparing the translation
elongation factor 1a gene. The results revealed the presence of FOE from approximately
80% of the isolates. Cameroonian isolate within FOE clade 1 exhibited the greatest
variability grouping with isolates from Suriname, Brazil and Democratic Republic of Congo.
Other isolates found in FOE clade 2 formed a unique group which was comprised solely
of isolates originating from Cameroon. Twenty-two isolates were chosen for pathogenicity
tests. After a short time, 14 isolates were found to be pathogenic to oil palm seedlings. This
study revealed the pathogenicity of FOE isolates from Cameroon and demonstrated that
FOE in Africa is more diverse than previously reported, including a lineage not previously
observed outside of Cameroon. Comparisons between all isolates will ultimately aid to devise
appropriate control mechanisms and better pathogen detection methods.
FUNDING
The first author acknowledges funding from the Fulbright
scholar grant and the Fungal Molecular Biology
Laboratory in the Department of Plant Pathology,
University of Florida, Gainesville, USA where the laboratory
work was carried out. The Cameroon Development
Corporation (CDC) gratefully acknowledges
personnel and material support through their Pest and
Disease Unit.
RESPONSIBLE EDITOR
Lidia Irzykowska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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