ORIGINAL ARTICLE
Molecular identification of Candidatus Phytoplasma spp. associated with Sophora yellow stunt in Iran
1
Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Mazandaran, Sari, Iran
2
Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Iran
Submission date: 2017-01-01
Acceptance date: 2017-06-05
Corresponding author
Mina Rastgou
Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Iran
Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Iran
Journal of Plant Protection Research 2017;57(2):167-172
KEYWORDS
TOPICS
ABSTRACT
In the spring of 2012, sophora (Sophora alopecuroides L.) plants showing symptoms of leaf yellowing, little leaves and stunting were observed in Firooz-kuh (Tehran province), Sari (Mazandaran province) and Urmia (West Azerbaijan province) in Iran. Symptomatic plants from the three locations were subjected to nested polymerase chain reaction (PCR) to amplify 16SrRNA using primer pair P1/P7 followed by primer pair R16F2n/R16R2. The amplicons were purified, sequenced and the nucleotide sequences were analyzed by virtual restriction fragment length polymorphism (RFLP). The phytoplasmas associated with the yellows disease were identified as members of the 16SrIX group (Candidatus Phytoplasma phoenicium) and the 16SrXII group (Candidatus Phytoplasma solani). The two phytoplasmas were placed in 16SrIX-C and 16SrXII-A subgroups, respectively, in constructed phylogenetic trees. This is the first report on sophora yellows associated with Candidatus Phytoplasma phoenicium.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (38)
1.
Abou-Jawdah Y., Sobh H., Akkary M. 2009. First report of Almond witches’ broom phytoplasma (‘Candidatus Phytoplasma phoenicium’) causing a severe disease on nectarine and peach trees in Lebanon. Bulletin OEPP/EPPO Bulletin 39 (1): 94–98. DOI: https://doi.org/10.1111/j.1365....
2.
Allahverdipoor T., Rahimian H., Rastgou M., Babaiezad V. 2013a. Molecular identification of the phytoplasma associated with peach and nectarine trees in West Azarbaijan province. 2th National Conference on Climate Change and its Impact on Agriculture and Nature, 14 August: 65–70.
3.
Allahverdipoor T., Rahimian H., Babaiezad V., Rastgou M. 2013b. Candidatus Phytoplasma solani associated with European and Japanese plums leaf roll in north of Iran. National Student Congress of Biosciences, 25–26 September: 71–72.
4.
Allahverdi T., Rahimian H., Babaeizad V. 2014. First report of a Candidatus Phytoplasma solani isolate affecting Sophora alopecuroides in Iran. New Diseases Report 30: 22. DOI: https://doi.org/10.5197/j.2044....
5.
Arocha-Rosete Y., Zunnoon-Khan S., Krukovets I., Crosby W., Scott J., Bertaccini A., Michelutti R. 2011. Identification and molecular characterization of the phytoplasma associated with peach rosette-like disease at the Canadian Clonal Genebank based on the 16S rRNA gene analysis. Canadian Journal of Plant Pathology 33 (2): 127–134. DOI: https://doi.org/10.1080/070606....
6.
Bayat H., Verhoeven T.Th .J., Botermans M., Peters D., Hassani-Mehraban A. 2013. First report of a 16SrIX group (‘Candidatus Phytoplasma phoenicium’-Related) phytoplasma associated with a chrysanthemum disease. Plant Disease 97 (8): 1110. DOI: https://doi.org/10.1094/pdis-0....
7.
Bertaccini A., Bellardi M.G., Vibio M. 1996. Virus diseases of ornamental shrubs. X. Euphorbia pulcherrima willd. Infected by viruses and phytoplasmas. Phytopathologia Mediterranea 35 (2): 129–132.
8.
Bertaccini A., Duduk B., Paltrinieri S., Contaldo N. 2014. Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. American Journal of Plant Sciences 5 (12): 1763–1788. DOI: https://doi.org/10.4236/ajps.2....
9.
Chamberlain D.F. 1970. Sophora L. p. 11–12. In: “Flora of Turkey and the East Aegean Islands”. Vol. 3 (P.H. Davis, ed.). Edinburgh University Press, Edinburgh, 628 pp.
10.
Chen X.F., Liang Y.C., Chen N., Su W.M., Xiao H., Wang X., Zhu X.P. 2013. Molecular identification of a phytoplasma associated with sophora root yellows. Forest Pathology 43: 415–421. DOI: https://doi.org/10.1111/efp.12....
11.
Davis R.E., Dally E.L., Gundersen D.E., Lee I.-M. Habili N. 1997. ‘Candidatus Phytoplasma australiense’, a new phytoplasma taxon associated with Australian grapevine yellows. International Journal of Systematic Bacteriology 47 (2): 262–269. DOI: https://doi.org/10.1099/002077....
12.
Davis R.E., Dally E.L., Zhao Y., Lee I-M., Jomantiene R., Detweiler A.J., Putnam M.L. 2010. First report of a new subgroup 16SrIX-E (‘Candidatus phytoplasma phoenicium’-related) phytoplasma associated with juniper witches’ broom disease in Oregon, USA. Plant Pathology 59 (6): 1161. DOI: https://doi.org/10.1111/j.1365....
13.
Duduk B., Bertaccini A. 2011. Phytoplasma classification: Taxonomy based on 16S ribosomal gene, is it enough? Phytopathogenic Mollicutes 1 (1): 3–13. DOI: https://doi.org/10.5958/j.2249....
14.
Duduk B., Tian J., Contaldo N., Fan X., Paltrinieri S., Chen Q., Zhao Q., Bertaccini A. 2010. Occurrence of phytoplasmas related to stolbur and to ‘Candidatus Phytoplasma japonicum’ in woody host plants in China. Journal of Phytopathology 158 (2): 100–104. DOI: https://doi.org/10.1111/j.1439....
15.
Hosseini P., Bahar M., Madani G., Zirak L. 2011. Molecular characterization of phytoplasmas associated with potato purple top disease in Iran. Journal of Phytopathology 159 (4): 241–246. DOI: https://doi.org/10.1111/j.1439....
16.
Khan A.J., Al-Subhi A.M., Calari A., Al-Saady N.A., Bertaccini A. 2007. A new phytoplasma associated with witches’ broom of Cassia italica in Oman. Bulletin of Insectology 60 (2): 269–270.
17.
Kenyon L., Harrison N.A., Ashburner G.R., Boa E.R., Richardson P.A. 1998. Detection of a pigeon pea witches’-broom-related phytoplasma in trees of Gliricidia sepium affected by little-leaf disease in Central America. Plant Pathology 47 (5): 671–680. DOI: https://doi.org/10.1046/j.1365....
18.
Lee I-M., Hammond R.W., Davis R.E., Gundersen D.E. 1993. Universal amplification and analysis of pathogen 16SrDNA for classifi cation and identification of mycoplasma-like organisms. Phytopathology 83 (8): 834–842. DOI: https://doi.org/10.1094/phyto-....
19.
Li C.L., Du Y.J., Xiang B.C. 2013. Molecular detection and identification of Sophora alopecuroides witche’s broom phytoplasma. Xinjiang Agricultural Sciences 50: 92–99.
20.
Mall S., Rao G., Marcone C. 2010. Phytoplasma diseases of weeds, taxonomy and diversity. p. 87–108. In: “Recent Trends in Biotechnology and Microbiology” (R.K. Gaur, P. Sharma, R. Pratap, K.P Sharma, M. Sharma, R. Dwivedi, eds.). Nova Science Publishers, Inc. 231 pp.
21.
Maixner M., Ahrens U., Seemüller E. 1995. Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts and vector by a specifi c PCR procedure. European Journal of Plant Pathology 101 (3): 241–250. DOI: https://doi.org/10.1007/bf0187....
22.
Marcone C., Ragozzino A., Seemuller E. 1997. Detection and identification of phytoplasmas infecting vegetable, ornamental and foliage crops in southern Italy. Journal of Plant Pathology 79: 211–217.
23.
Mirchenari S.A., Massah A., Zirak L. 2015. ‘Bois noir’: new phytoplasma disease of grapevine in Iran. Journal of Plant Protection Research 55 (1): 88–93. DOI: https://doi.org/10.1515/jppr-2....
24.
Molino Lova M., Quaglino F., Abou-Jawdah Y., Choueiri E., Sobh H., Casati P., Tedeschi R., Alma A., Bianco P.A. 2011. Identification of new 16SrIX subgroups, -F and -G, among ‘Candidatus Phytoplasma phoenicium’ strains infecting almond, peach and nectarine in Lebanon. Phytopathologia Mediterranea 50: 273–282.
25.
Quaglino F., Zhao Y., Casati P., Bulgari D., Bianco P.A., Wei W., Davis R.E. 2013. ‘Candidatus Phytoplasma solani’, a novel taxon associated with stolbur- and Bois noir-related diseases of plants. International Journal of Systematic and Evolutionary Microbiology 63 (8): 2879–2894. DOI: https://doi.org/10.1099/ijs.0.....
26.
Salehi M., Izadpanah K., Nejat N. 2006. A new phytoplasma infecting lettuce in Iran. Plant Disease 90 (2): 247. DOI: https://doi.org/10.1094/pd-90-....
27.
Salehi M., Haghshenas F., Khanchezar A., Esmailzadeh-Hosseini S.A. 2011. Association of ‘Candidatus Phytoplasma phoenicium’ with GF-677 witches’ broom in Iran. Bulletin of Insectology 64: 113–114.
28.
Schneider B., Seemuller E., Smart C.D., Kirkpatrik B.E. 1995. Phylogenetic classification of plant pathogenic mycoplasma-like organisms or phytoplasmas. p. 369–380. In: “Molecular and Diagnostic Procedures in Mycoplasmalogy” (S. Razin, J.G Tully, eds.). Academic Press, 483 pp.
29.
Singh N., Upadhyaya P.P. 2013. Analysis of medicine weeds associated with phytoplasma by morphological characters. International Journal of Medicinal Plants and Alternative Medicine 2 (1): 001–004.
30.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, Evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28 (10): 2731–2739. DOI: https://doi.org/10.1093/molbev....
31.
Valiunas D., Staniulis J. Davis R. E. 2006. ‘Candidatus Phytoplasma fragariae’, a novel phytoplasma taxon discovered in yellows diseased strawberry, Fragaria x ananassa. International Journal of Systematic and Evolutionary Microbiology 56 (1): 277–281. DOI: https://doi.org/10.1099/ijs.0.....
32.
Verdin E., Salar P., Danet J., Choueiri E., Jreijiri F., El Zammar S., Gelie B., Bove J.M., Garnier M. 2003. ‘Candidatus Phytoplasma phoenicium’ sp. nov., a novel phytoplasma associated with an emerging lethal disease of almond trees in Lebanon and Iran. International Journal of Systematic and Evolutionary Microbiology 53 (3): 833–838. DOI: https://doi.org/10.1099/ijs.0.....
33.
Yu Z.C., Cao Y., Zhang Q., Deng D.F., Liu Z.Y. 2012. ‘Candidatus Phytoplasma ziziphi’ associated with Sophora japonica witches’ broom disease in China. Journal of General Plant Pathology 78 (4): 298–300. DOI: https://doi.org/10.1007/s10327....
34.
Zhao Y., Wei W., Lee I-M., Shao J., Suo X., Davis R.E. 2009. Construction of an interactive online phytoplasma classification tool, iPhyClassifi er, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology 59 (10): 2582–2593. DOI: https://doi.org/10.1099/ijs.0.....
35.
Zirak L., Bahar M., Ahoonmanesh A. 2009a. Characterization of phytoplasmas associated with almond diseases in Iran. Journal of Phytopathology 157 (11–12): 736–741. DOI: https://doi.org/10.1111/j.1439....
36.
Zirak L., Bahar M., Ahoonmanesh A. 2009b. Molecular characterization of phytoplasmas related to peanut witches-broom and stolbur groups infecting plum in Iran. Journal of Plant Pathology 91 (3): 713–716. DOI: http://dx.doi.org/10.4454/jpp.....
37.
Zirak L., Bahar M., Ahoonmanesh A. 2010. Molecular characterization of phytoplasmas associated with peach diseases in Iran. Journal of Phytopathology 158 (2): 105–110. DOI: https://doi.org/10.1111/j.1439....
38.
Zibadoost S., Rastgou M. 2016. Molecular identification of phytoplasmas associated with some weeds in West Azarbaijan province, Iran. Acta Agriculturae Slovenica 107 (1): 129. DOI: https://doi.org/10.14720/aas.2....
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