ORIGINAL ARTICLE
Molecular detection of Cucumber mosaic virus from Basella alba, Telfairia occidentalis and Talinum fruticosum in Nigeria
1
Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-09-14
Acceptance date: 2019-05-29
Online publication date: 2019-06-27
Corresponding author
Adedapo Olutola Adediji
Department of Crop Protection and Environmental Biology, University of Ibadan, Agbowo, 200222, Ibadan, Nigeria
Department of Crop Protection and Environmental Biology, University of Ibadan, Agbowo, 200222, Ibadan, Nigeria
Journal of Plant Protection Research 2019;59(2):177-184
KEYWORDS
TOPICS
ABSTRACT
Cucumber mosaic virus (CMV; family Bromoviridae, genus Cucumovirus) is the most
cosmopolitan plant virus occurring worldwide. In the present study, leaf samples showing
deformations, mosaics, and chlorotic spots symptoms were collected from naturally
infected Basella alba, Telfairia occidentalis and Talinum fruticosum in a home yard garden
in Ibadan, Nigeria. Total nucleic acid was extracted from leaves and used as template for
cDNA synthesis. RT-PCR was carried out using CMV-specific primers targeting RNA-1
segment. Samples were also tested by RT-PCR using Potyvirus and Begomovirus genusspecific
primers. DNA fragments with the expected sizes of ~500 bp were amplified by
using CMV-specific primers; however, the expected amplicons were not produced using
specific primers used for the detection of potyviruses and begomoviruses. The nucleotide
and deduced amino acid sequences obtained for the isolates studied contained 503–511 nt
and 144 aa, respectively. The isolates shared 81.9–85.3% nucleotide and 74.3–77.8% amino
acid sequence identities with each other. The results of BLASTN analyses showed the
highest identities of the isolates (80–93%) with CMV strains from Japan, USA and South
Korea. Alignment of deduced partial protein revealed multiple amino acid substitutions
within the three isolates and high identities with CMV subgroup I. Phylogenetic analyses
putatively categorized the isolates in close association with subgroup IB isolates. The three
isolates clustered together into a separate subclade, indicating possible new CMV strains.
The results provide the first molecular evidence for CMV infections of T. fruticosum and
B. alba in Nigeria and seem to show the possible presence of new strain(s). These findings
also add three new hosts to the list of natural host range of the virus in Nigeria.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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