ORIGINAL ARTICLE
Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan
1
Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, 45500 Islamabad, Pakistan
2
Faculty of Agricultural Sciences and Technology, Department of Plant Pathology, Bahauddin Zakariya University, 60800 Multan, Pakistan
3
Faculty of Agricultural Sciences and Technologies, Department of Plant Protection, Sivas University of Science and Technology, 58140 Sivas, Turkey
4
Department of Food Science and Technology, Bahauddin Zakariya University, 60000 Multan, Pakistan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-09-12
Acceptance date: 2024-02-14
Online publication date: 2024-05-13
Corresponding author
Hajra Azeem
Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, 45500 Islamabad, Pakistan
Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, 45500 Islamabad, Pakistan
Journal of Plant Protection Research 2024;64(2):139-148
HIGHLIGHTS
- Begomovirus
- Chilli
- Virus on chilli
- Cotton leaf curl virus
- Cotton leaf curl Multan betasatellite
- Geminiviridae
- Phylogenetic analysis
- Leaf curling
- Stunted growth
KEYWORDS
TOPICS
ABSTRACT
The prevalence of cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum
spp.) production, presenting a formidable challenge in Pakistan. During a chili field
survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf
curling, were observed on several plants. Subsequently, a comprehensive sampling effort
was undertaken, collecting a total of 39 symptomatic samples from diverse locations across
Multan, Punjab. The DNA extraction from these samples was conducted at the plant virology
laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the
investigation of this debilitating disease and its impact on chili production in the region.
Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers
confirmed begomovirus infection in chili plants. Positive amplification demonstrated
a 71.79% infection rate, with 579 bp, 1.4 kb, and 481 bp amplicons for Av/Ac Core, Beta
01/02, and CLCuMuBF11/R33, respectively. Sequencing identified cotton leaf curl Multan
beta-satellite (MT668934) infecting the chili plant. Effectively managing these begomoviruses
is crucial to curbing their multiplication and protecting vital crops like chili. Addressing
the distributions of beta-satellites in agricultural fields, particularly chili, is imperative
to prevent further viral spread.
RESPONSIBLE EDITOR
Huan Zhang
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (61)
1.
Abdel-Salam A., Mujaddad-ur-Rehman M., El-Saghir S., El-Shazly M. 2016. Molecular characterization of a sweet potato leaf curl virus isolate from Egypt and it’s phylogenetic relationship with other members of the Begomovirus. British Microbiology Research Journal 17: 1–8. DOI: https://doi.org/10.9734/BMRJ/2....
2.
Abdullah A., Ahmed A., Akhter P., Razzaq A., Hussain M., Hossain N., Bakar M.S.A., Khurram S., Majeed K., Park Y.K. 2021. Potential for sustainable utilisation of agricultural residues for bioenergy production in Pakistan: An overview. Journal of Cleaner Production 287: 125047. DOI: https://doi.org/10.1016/j.jcle....
3.
Afzal M., Saeed S., Riaz H., Ishtiaq M., Habib ur Rahman M. 2023a. Transmission efficiency of cotton leaf curl Khokhran virus/cotton leaf curl Multan beta-satellite complex by two whitefly cryptic species in Pakistan. International Journal of Tropical Insect Science (2023) 43:819–830. DOI: https://doi.org/10.1007/s42690....
4.
Afzal M., Saeed S., Riaz H., Ishtiaq M., Rahman M.H. 2023b. A critical review of whitefly (Bemisia tabaci gennadius) cryptic species associated with the cotton leaf curl disease. Journal of Innovative Science 9: 24–43. DOI: https://dx.doi.org/10.17582/jo....
5.
Akhtar K.P., Ullah R., Saeed M., Sarwar N., Mansoor S. 2014a. China rose (Hibiscus rosa-sinensis): a new natural host of cotton leaf curl Burewala virus in Pakistan. Journal of Plant Pathology 96: 385–389.
6.
Akhter A., Akhtar S., Saeed M., Mansoor S. 2014b. Chilli leaf curl Betasatellite enhances symptoms induced by tomato leaf curl New Delhi virus, a bipartite Begomovirus. International Journal Of Agriculture & Biology: 1225–1228.
7.
Akram M., Kamaal N., Pratap A., Muin A., Agnihotri A.K., Rathore U.S., Rathore M., Singh N.P. 2022. Species diversity, phylogeny and evidence of recombination in begomoviruses associated with yellow mosaic disease of moth bean (Vigna aconitifolia) in South India. Journal of Phytopathology 170 (5): 300–314. DOI: https://doi.org/10.1111/jph.13....
8.
Ali I., Khurshid M., Iqbal Z., Shafiq M., Amin I., Mansoor S., Briddon R.W. 2019. The antisense 5'end of the V2 gene confers enhanced resistance against themonopartitebegomovirus cotton leaf curl Kokhran virus-Burewala strain. Acta Virologica 63: 3–12. DOI: 10.4149/av_2019_101.
9.
Ali N.F., Paracha R.Z., Tahir M. 2021. In silico evaluation of molecular virus–virus interactions taking place between Cotton leaf curl Kokhran virus-Burewala strain and tomato leaf curl New Delhi virus. PeerJ 9: 12018. DOI: https://doi.org/10.7717/peerj.....
10.
Ali A., Zeshan M.A., Iftikhar Y., Abid M., Ehsan S.F., Ghani M.U., Khan A.A. 2020. Role of plant extracts and salicylic acid for the management of chili veinal mottle virus disease. Pakistan Journal of Phytopathology 32 (2): 147–157. DOI: 10.33866/phytopathol.030.02.0583.
11.
Amrao L., Akhter S., Tahir M.N., Amin I., Briddon R.W., Mansoor S. 2010. Cotton leaf curl disease in Sindh province of Pakistan is associated with recombinant begomovirus components. Virus Research 153: 161–165. DOI: https://doi.org/10.1016/j.viru....
12.
Arin S. 2019. Scenario of chilli production and hindrances faced by the growers of Sindh province of Pakistan. Modern Concept and Development in Agronomy 4 (3): 436–442. DOI: 10.31031/MCDA.2019.04.000588.
13.
Azeem H., Perveen R., Tahir M.N., Umar U.U.D., Ölmez F., Ali A. 2022. Prevalence, transmission and molecular characterization of cotton leaf curl Multan virus infecting hollyhock plants in Pakistan. Molecular Biology Reports (2022) 49: 5635–5644. DOI: https://doi.org/10.1007/s11033....
14.
Azlan A., Sultana S., Huei C.S., Razman M.R. 2022. Antioxidant, anti-obesity, nutritional and other beneficial effects of different chili pepper: a review. Molecules 27 (3): 898. DOI: https://doi.org/10.3390/molecu....
15.
Baig M.S., Akhtar S., Khan J.A. 2021. Engineering tolerance to CLCuD in transgenic Gossypium hirsutum cv. HS6 expressing cotton leaf curl Multan virus-C4 intron hairpin. Science Report 11 (1): 14172. DOI: https://doi.org/10.1038/s41598....
16.
Balram N., Bhattacharyya U.K., Aman S., Pradeep K., Sandeep R., Rupesh A., Anil K., Palchoudhury S., Dilip M., Biswas K.K. 2017. Current distribution and severity of leaf curl begomovirus disease in cotton growing areas of Northwest India. Journal of Mycology and Plant Pathology 47: 237–251.
17.
Briddon R.W., Markham P.G. 2000a. Cotton leaf curl virus disease. Virus Research 71 (1–2): 151–159. DOI: https://doi.org/10.1016/S0168-....
18.
Briddon R.W., Bull S.E., Mansoor S., Amin I., Markham P.G. 2002b. Universal primers for the PCR-mediated amplification of DNA β: a molecule associated with some monopartite begomoviruses. Molecular Biotechnology 20: 315–318. DOI: 1073–6085/2002/20:3/315–318/$11.00.
19.
Briddon R.W., Stanley J. 2006c. Sub-viral agents associated with plant infecting single-stranded DNA viruses. Virology 344: 198–210. DOI: https://doi.org/10.1016/j.viro....
20.
Brown J.K., Ur-Rehman M.Z., Avelar S., Chingandu N., Hameed U., Haider S., Ilyas M. 2017. Molecular diagnostic development for begomovirus-betasatellite complexes undergoing diversification: A case study. Virus Research 241: 29–41. DOI: https://doi.org/10.1016/j.viru....
21.
Chakrabarty P.K., Kumar P., Kalbande B.B., Chavhan R.L., Koundal V., Monga D., Pappu H.R., Roy A., Mandal B. 2020. Recombinant variants of cotton leaf curl Multan virus is associated with the breakdown of leaf curl resistance in cotton in northwestern India. Virus Disease 31: 45–55. DOI: https://doi.org/10.1007/s13337....
22.
Chakraborty P., Ghosh A. 2022. Topical spray of dsRNA induces mortality and inhibits chilli leaf curl virus transmission by Bemisia tabaci Asia II 1. Cells 11 (5): 833. DOI: https://doi.org/10.3390/cells1....
23.
Chen T., Saeed Q., He Z., Lu L. 2019. Transmission efficiency of cotton leaf curl Multan virus by three cryptic species of Bemisia tabaci complex in cotton cultivars. PeerJ 7 (3): 1–13. DOI: http://doi.org/10.7717/peerj.7....
24.
Doyle J.J., Doyle J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12 (1): 13–15.
25.
Farooq T., Umar M., She X., Tang Y., He Z. 2021. Molecular phylogenetics and evolutionary analysis of a highly recombinant begomovirus, cotton leaf curl Multan virus, and associated satellites. Virus Evolution 7 (2): 1–15. DOI: https://doi.org/10.1093/ve/vea... 054.
26.
Fiallo‐Olivé E., Tovar R., Navas‐Castillo J. 2016. Deciphering the biology of deltasatellites from the New World: maintenance by New World begomoviruses and whitefly transmission. New Phytologist 212: 680–692. DOI: https://doi.org/10.1111/nph.14....
27.
Fondong V.N. 2013. Geminivirus protein structure and function. Molecular Plant Pathology 14: 635–649. DOI: https://doi.org/10.1111/mpp.12....
28.
Gnanasekaran P., Kishore Kumar R., Bhattacharyya D., Vinoth Kumar R., Chakraborty S. 2019. Multifaceted role of geminivirus associated betasatellite in pathogenesis. Molecular Plant Pathology 20: 1019–1033. DOI: https://doi.org/10.1111/mpp.12....
29.
Gupta K., Rishishwar R., Khan Z.A., Dasgupta I. 2022. Agrobacterium-mediated co-inoculation of okra plants with cloned okra enation leaf curl virus DNA and bhendi yellow vein mosaic beta-satellite DNA furthers Koch’s postulates for enation leaf curl disease. Journal of Virological Method 300: 114413. DOI: https://doi.org/10.1016/j.jvir....
30.
Hassan I., Amin I., Mansoor S., Briddon R.W. 2017. Further changes in the cotton leaf curl disease complex: an indication of things to come? Virus Genes 53: 759–761. DOI: 10.1007/s11262-017-1496-1.
31.
Hyder S., Inam-ul-Haq M., Ashfaq M., Ahmad A., Gondal A.S., Iqbal M. 2018. First report of Pythium myriotylum D., causing damping off and root rot in chili pepper (Capsicum annum L.) from Punjab, Pakistan. Plant Disease 102: 687–687. DOI: https://doi.org/10.1094/PDIS-0....
32.
Ijaz S., Mubin M., Nawaz-Ul-Rehman M.S., Khan A.A. 2020. Molecular analysis of Pedilanthus leaf curl virus and associated satellites infecting Chenopodium album in Pakistan. Pakistan Journal of Agricultural Science 57: 425–432. DOI: 10.21162/PAKJAS/20.8491.
33.
Jyothsna P., Haq Q.M.I., Singh P., Sumiya K.V., Praveen S., Rawat R., Briddon R.W., Malathi V.G. 2013. Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites. Applied Genetics And Molecular Biotechnology 97: 5457–5471. DOI: 10.1007/s00253-012-4685-9.
34.
Khan Z.A., Khan J.A. 2017. Characterization of a new begomovirus and betasatellite associated with chilli leaf curl disease in India. Archives of Virology 162: 561–565. DOI: 10.1007/s00705-016-3096-0.
35.
Khan A.J., Akhtar S., Al-Zaidi A.M., Singh A.K., Briddon R.W. 2013.Genetic diversity and distribution of a distinct strain of Chili leaf curl virus and associated betasatellite infecting tomato and pepper in Oman. Virus Res 177: 87–97. DOI: https://doi.org/10.1016/j.viru....
36.
Kumar A., Snehi S.K., Raj S.K., Kumar J., Khan J.A. 2011. Association of cotton leaf curl Burewala virus and its satellite molecules with leaf distortion symptoms of cotton in India. New Disease Report 24: 2044–0588. DOI: 10.5197/j.2044-0588.2011.024.018.
37.
Kumar S., Stecher G., Li M., Knyaz C., Tamura K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biologyand Evolution 35 (6): 1547. DOI: 10.1093/molbev/msy096.
38.
Kumar Y., Hallan V., Zaidi A.A. 2011. Chilli leaf curl Palampur virus is a distinct begomovirus species associated with a betasatellite. Plant Pathology 60: 1040–1047. DOI: https://doi.org/10.1111/j.1365....
39.
Mansoor S., Briddon R.W., Bull S.E., Bedford I.D., Bashir A., Hussain M., Saeed M., Zafar Y., Malik K.A., Fauquet C., Markham P.G. 2003. Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA β. Archives of Virology 148: 1969–1986. DOI: 10.1007/s00705-003-0149-y.
40.
Melgarejo T.A., Kon T., Rojas M.R., Paz-Carrasco L., Zerbini F.M., Gilbertson R.L. 2013. Characterization of a new world monopartite begomovirus causing leaf curl disease of tomato in Ecuador and Peru reveals a new direction in geminivirus evolution. Journal of Virology 87: 5397–5413. DOI: https://doi.org/10.1128/jvi.00....
41.
Mubin M., Amin I., Amrao L., Briddon R.W., Mansoor S. 2010. The hypersensitive response induced by the V2 protein of a monopartite begomovirus is countered by the C2 protein. Molecular Plant Pathology 11: 245–254. DOI: https://doi.org/10.1111/j.1364....
42.
Naqvi R.Z., Zaidi S.S.E.A., Mukhtar M.S., Amin I., Mishra B., Strickler S., Mueller L.A., Asif M., Mansoor S. 2019. Transcriptomic analysis of cultivated cotton Gossypium hirsutum provides insights into host responses upon whitefly-mediated transmission of cotton leaf curl disease. PloS One 14: e0210011. DOI: https://doi.org/10.1371/journa....
43.
Nigam D. 2021. Genomic variation and diversification in begomovirus genome in implication to host and vector adaptation. Plants 10: 1706. DOI: https://doi.org/10.3390/plants....
44.
Nigam K., Srivastava S., Kumar D., Verma Y., Gupta S. 2014. Detection and analysis of leaf curl virus from Jatropha. International Journal Scientific and Research Publication 4 (05): 538–544.
45.
Rahman M.U., Khan A.Q., Rahmat Z., Iqbal M.A., Zafar Y. 2017. Genetics and genomics of cotton leaf curl disease, its viral causal agents and whitefly vector: a way forward to sustain cotton fiber security. Frontiers in Plant Science 8: 1157. DOI: https://doi.org/10.3389/fpls.2....
46.
Rai V.P., Kumar R., Singh S.P., Kumar S., Kumar S., Singh M., Rai M. 2014. Monogenic recessive resistance to pepper leaf curl virus in an interspecific cross of Capsicum. Scientia Horticulturae 172: 34–38. DOI: https://doi.org/10.1016/j.scie....
47.
Rais M.N., Mangan T., Sahito J.G.M., Qureshi N.A., Sial M.U., Nasir A. 2021. An empirical analysis of constraints faced by chilli growers and market intermediaries in production and marketing of chilli in Sindh, Pakistan. Al Arch's Journal of Archaeology of Egypt/ Egyptology 18: 1485–1491.
48.
Rajagopalan P.A., Naik A., Katturi P., Kurulekar M., Kankanallu R.S., Anandalakshmi R. 2012. Dominance of resistance-breaking cotton leaf curl Burewala virus (CLCuBuV) in northwestern India. Archieves of Virology 157: 855–868. DOI: 10.1007/s00705-012-1225-y.
49.
Sahid Z.D., Syukur M., Maharijaya A., Nurcholis W. 2022. Total phenolics, flavonoids, antioxidant activity, and α-glucosidase inhibitory activity of ornamental pepper and several other lines. Ornamental Horticulture 28: 230–238. DOI: https://doi.org/10.1590/2447-5....
50.
Saleem H., Nahid N., Arshad M., Murtaza G. 2020. First report of tomato leaf curl New Delhi virus and cotton leaf curl Multan betasatellite on pepper in Pakistan. Plant Disease 104 (7): 2007. DOI: 10.1111/j.1365-3059.2005.01278.x.
51.
Sattar M.N., Iqbal Z., Tahir M.N., Ullah S. 2017. The prediction of a new CLCuD epidemic in the Old World. Frontiers in Microbiology 8: 631. DOI: https://doi.org/10.3389/fmicb.....
52.
Sattar M.N., Kvarnheden A., Saeed M., Briddon R. . 2013. Cotton leaf curl disease – an emerging threat to cotton production worldwide. Journal of General Virology 94 (4): 695–710. DOI: https://doi.org/10.1099/vir.0.....
53.
Senanayake D.M.J.B., Varma A. Mandal B. 2012. Virus–vector relationships, host range, detection and sequence comparison of Chilli leaf curl virus associated with an epidemic of leaf curl disease of chilli in Jodhpur. Indian Journal of Phytopathology 160: 146–155. DOI: https://doi.org/10.1111/j.1439....
54.
Shakir S., Zaidi S.S.E.A., Farooq M., Amin I., Scheffler J., Scheffler B., Shah Nawaz-ul-Rehman M. Mansoor S. 2019. Non-cultivated cotton species (Gossypium spp.) act as a reservoir for cotton leaf curl begomoviruses and associated satellites. Plants 8: 127. DOI: https://doi.org/10.3390/plants....
55.
Sharma D., Kulshreshtha A., Roshan P., Hallan V. 2019. Molecular characterization and infectivity analysis of a bipartite begomovirus associated with cotton leaf curl Multan betasatellite naturally infecting Rumex nepalensis in northern India. Journal of Plant Pathology 101: 935–941. DOI: https://doi.org/10.1007/s42161....
56.
Shih S.L., Kumar S., Tsai W.S., Lee L.M., Green S.K. 2009. Complete nucleotide sequences of okra isolates of Cotton leaf curl Gezira virus and their associated DNA-β from Niger. Archives of Virology 154: 369–372. DOI: 10.1007/s00705-008-0304-6.
57.
Sinha V., Kumar A., Sarin N.B., Bhatnagar D. 2016. Recombinant croton yellow vein mosaic virus associated with severe leaf curl disease of papaya in India. International Journal of Advanced Research 4 (03): 1598–1604.
58.
Sohrab S.S., Kamal M.A., Ilah A., Husen A., Bhattacharya P.S., Rana D. 2016. Development of cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene. Saudi Journal of Biological Sciences 23: 358–362. DOI: https://doi.org/10.1016/j.sjbs....
59.
Tahir M.N., Mansoor S. 2011. bC1 of chili leaf curl betasatellite is a pathogenicity determinant. Virol J 8: 509. DOI: http://www.virologyj.com/conte....
60.
Tabein S., Tahmasebi A., Behjatnia S.A.A. 2022. Induction of disease symptoms of a non-cognate helper begomovirus by CLCuMB βC1 through the salicylic acid-dependent pathway. Journal of Plant Biochemistry and Biotechnology 32 (2): 356–362. DOI: https://doi.org/10.1007/s13562....
61.
Zubair M., Zaidi S.S.E.A., Shakir S., Amin I., Mansoor S.2017. An insight into cotton leaf curl Multan betasatellite, the most important component of cotton leaf curl disease complex. Viruses 9: 280. DOI: https://doi.org/10.3390/v91002....
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
