ORIGINAL ARTICLE
Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.)
1
Rice and Industrial Crops Research Centre, MARDI Seberang Perai, P.O. Box 203, 13200 Kepala Batas, Penang, Malaysia
2
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
Submission date: 2015-01-17
Acceptance date: 2015-10-30
Corresponding author
Latiffah Zakaria
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia
Journal of Plant Protection Research 2015;55(4):396-404
KEYWORDS
TOPICS
ABSTRACT
The
Erwinia
species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value
fruit crops that include pineapple [Ananas comosus
(L.) Merr.] and papaya (Carica papaya
L.).The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species;
Dickeya zeae
from pineapple heart rot, and
Erwinia mallotivora
from papaya
dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own
taxa and formed monophyletic clades. From the pathogenicity test, all isolates of
D. zeae
and
E. mallotivora
showed pathogenic reactions on their respective host plants. Genetic variability of these
isolates was assessed using repetitive sequence-based PCR (rep-PCR)
fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic
bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC) and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA) cluster
analysis indicated that all
Dickeya
and
Erwinia
isolates from the same species were grouped in the same main cluster. Similarity among
the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among
Dickeya
and
Erwinia
isolates. But this method appears to be reliable for discriminating isolates from pineapple
heart rot and papaya dieback
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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