ORIGINAL ARTICLE
Marker-assisted selection for scald (Rhynchosporium commune L.)
resistance gene(s) in barley breeding for dry areas
1
Institute of Molecular, Cell and System Biology, University of Glasgow, Glasgow, United Kingdom
2
Biodiversity and Integrated Gene Management Program, The International Centre for Agricultural Research in Dry Areas (ICARDA), Aleppo, Syria
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-03-28
Acceptance date: 2018-10-10
Online publication date: 2018-11-23
Corresponding author
Haitham Sayed
Institute of Molecluar, Cell and System Biology, University of Glasgow, Stevenson Laboratory, Bower Building, University of Glasgow, Glasgow, G12 8QQ Glasgow, United Kingdom
Institute of Molecluar, Cell and System Biology, University of Glasgow, Stevenson Laboratory, Bower Building, University of Glasgow, Glasgow, G12 8QQ Glasgow, United Kingdom
Journal of Plant Protection Research 2018;58(4):335-344
KEYWORDS
TOPICS
ABSTRACT
Barley scald, caused by Rhynchosporium commune is one of the most prevalent diseases
in barley (Hordeum vulgare L.) worldwide. The primary loss from scald is reduced yield,
which can exceed 25% in dry areas. In our earlier studies, we developed a low-resolution
linkage map for recombinant inbred lines of the cross Tadmor/WI2291. Quantitative trait
loci (QTLs) for scald were localized on chromosomes 2H and 3H flanked by Simple Sequence
Repeat (SSR) markers HVM54 and Bmac0093b on 2H and HVLTPP8, HVM62 and
Bmag0006 on 3H. These chromosome 3H markers were found to be located close to the
Rrs1 − R. commune resistance gene(s) on chromosome 3H. In this study, 10 homozygous
resistant and 10 homozygous susceptible plants each from the F7 population of Tadmor/
Sel160, a panel of 23 barley varieties used routinely in the International Centre for Agricultural
Research in the Dry Areas (ICARDA) breeding program and three populations were
used for scald resistance screening using 25 DNA markers that are located very close to scald
resistance gene(s) on barley chromosomes. Only five of those markers clearly discriminated
co-dominantly between resistant and susceptible plants. These markers, Ebmac0871-
SSR, HVS3-SCAR, Bmag0006-SSR, reside on different arms of barley chromosome 3H.
Ebmac871 is localized on the short arm of 3H and HVS3 and Bmag0006 are localized on
the long arm of 3H. This result indicates that the scald resistance genes which they tag are
probably close to the centromeric region of this chromosome. Scald resistance from several
sources map to the proximal region of the long arm of chromosome 3H, forming the complex
Rrs1 locus. The availability of highly polymorphic markers for the discrimination of
breeding material would be extremely useful for barley breeders to select for the trait at the
DNA level rather than relying on phenotypic expression and infection reaction.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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