ORIGINAL ARTICLE
Isolation and characterization of tenuazonic acid produced by Alternaria alternata, a potential bioherbicidal agent for control of Lantana camara
1
Research and Development Centre, Bisen Biotech & Biopharma Pvt. Ltd., Biotech Research Park, M-7, Laxmipuram, Transport Nagar, Gwalior – 474010 (M.P.), India
2
Mycological Research Laboratory, Rani Durgavati University, Jabalpur – 482001 (M.P.), India
3
School of Studies in Biotechnology, Jiwaji University, Gwalior – 474011 (M.P.), India
Corresponding author
Sanodiya1 Bhagwan Singh
Research and Development Centre, Bisen Biotech & Biopharma Pvt. Ltd., Biotech Research Park, M-7, Laxmipuram, Transport Nagar, Gwalior – 474010 (M.P.), India
Research and Development Centre, Bisen Biotech & Biopharma Pvt. Ltd., Biotech Research Park, M-7, Laxmipuram, Transport Nagar, Gwalior – 474010 (M.P.), India
Journal of Plant Protection Research 2010;50(2):133-139
KEYWORDS
TOPICS
ABSTRACT
Microbial preparation of herbicide is defined as bioherbicide that can control the weed. In this approach, indigenous plant pathogens isolated from weeds are cultured to produce the large numbers of infective propagules which are applied at a rate that will cause high levels of infection leading to suppression of the target weed. During the present investigation, cell free culture filtrate
(CFCF) of Alternaria alternata was evaluated for its phytotoxicity against a noxious weed Lantana camara. The results of cut shoot, seedling and detached leaf bioassays revealed the presence of a toxic metabolite in the CFCF and a significant reduction in chlorophyll and protein content were also noticed. Phytotoxic moiety was further purified and characterized by using solvent partition, thin layer chromatography (TLC), FTIR and 1H NMR analysis. The acetone extract induced maximum phytotoxic damage at a concentration of 100 μg/ml and TLC purified fraction also exhibited herbicidal potential. The toxic compound was identified as tenuazonic acid upon
comparison with FTIR and 1H NMR spectra. This is the first evidence that confirmed the herbicidal potential of a biorational, tenuazonic acid was produced by submerged fermentation of A. alternata.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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