ORIGINAL ARTICLE
Invasive mealybug Pseudococcus jackbeardsleyi Gimpel and Miller on Curcuma aeruginosa Roxb. on the seed rhizome in storage: a record of a new host
1
Research Center for Estate Crops, National Research and Innovation Agency (Badan Riset dan Inovasi Nasional – BRIN), Cibinong, Indonesia
2
Department of Plant Protection, Faculty of Agriculture, IPB University, Bogor, Indonesia
3
Department of Plant Production, Politeknik Negeri Jember, Jember, Indonesia
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-02-08
Acceptance date: 2024-04-02
Online publication date: 2025-12-03
Corresponding author
Miftakhurohmah
Research Center for Estate Crops, National Research and Innovation Agency (Badan Riset dan Inovasi Nasional-BRIN), Jl. Raya Jakarta-Bogor KM. 46,, 16915, Cibinong, Bogor,, Indonesia
Research Center for Estate Crops, National Research and Innovation Agency (Badan Riset dan Inovasi Nasional-BRIN), Jl. Raya Jakarta-Bogor KM. 46,, 16915, Cibinong, Bogor,, Indonesia
Journal of Plant Protection Research 2024;64(4):298-306
HIGHLIGHTS
- First report of the presence of Pseudococcus jackbeardsleyi in Curcuma aeruginosa rhizomes in storage in Indonesia
- Identification of P. jackbeardsleyi based on morphological and molecular characters
- Mealybug damage the quality and quantity of rhizomes and have the potential to spread to other hosts during storage
- Due to its wide host range, quick action is needed when a P. jackbeardsleyi attack appears.
KEYWORDS
TOPICS
ABSTRACT
Mealybug is a renowned pest known to attack agricultural products from the field to the
post-harvest process, such as on the seed rhizomes of Curcuma aeruginosa. Therefore,
this study aimed to examine and identify the species of mealybug on the seed rhizomes of
C. aeruginosa based on morphological and molecular characteristics. Fifty mealybugs were
collected from the seed rhizomes of C. aeruginosa in the storage room in Bogor (Indonesia)
using a soft brush. They were transferred to new C. aeruginosa rhizomes without
any other insects present. Morphological identification based on observation of mounted
specimens of 10 female adults and six for molecular identification. The primer pair that
amplified the mitochondrial cytochrome oxidase I (COI) gene was used to study the molecular
characteristics and was continued with direct sequencing and sequence analysis.
The results showed that the morphological characteristics of the mounted specimen were
close to those of Pseudococcus jackbeardsleyi. Amplification of the COI gene yielded DNA
bands measuring 490 base pairs (bp), while homology and phylogeny analysis confirmed
the morphological identification. Based on BLAST analysis, the similarity of COI genes of
mealybugs in this study was above 99% with other P. jackbeardsleyi. The study specimen
was identified as P. jackbeardsleyi on the seed rhizomes of C. aeruginosa by comparing the
morphological features of insect specimens and results of the species available in Gene-
Bank. This result represented the first documented report about the presence of the species
in storage.
ACKNOWLEDGEMENTS
The authors acknowledge the contribution of the Indonesian
Spice and Medicinal Crops Research Institute for
facilitating the collection, mass rearing, and identification
of the insect. The authors also wish to thank Betti
Sumaryati for assisting with specimen preparation.
RESPONSIBLE EDITOR
Jacek Twardowski
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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