ORIGINAL ARTICLE
Induction of systemic resistance to Orobanche crenata in lentil by exogenous application of salicylic acid and indole acetic acid
1
Biotechnology Research Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research, Rabat, Morocco
2
Department of Biotechnology and Plant Physiology, Faculty of Sciences, Mohammed V University, Rabat, Morocco
3
Department of Crop Production, Protection and Biotechnology, Institute of Agronomy and Veterinary Medicine Hassan II, Rabat, Morocco
4
Agro-sciences (AgBS), University Mohammed VI Polytechnic (UM6P), Benguerir, Morocco
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-09-21
Acceptance date: 2022-11-21
Online publication date: 2023-03-15
Corresponding author
Rachid Mentag
Biotechnology Research Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research, Rabat, Morocco
Biotechnology Research Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research, Rabat, Morocco
Journal of Plant Protection Research 2023;63(1):83-96
HIGHLIGHTS
- Salicylic acid (SA) must be applied at concentration of 1 mM
- Indole acetic acid (IAA) must be applied at concentration of 0.09 mM
- SA and IAA treatments reduced O. crenata infestation in lentil by activation of SAR
- IAA acts as growth hormone and as inducer of systemic resistance (SAR) in lentil
- SA and IAA treatments increased activities of phenol metabolizing enzymes
KEYWORDS
TOPICS
ABSTRACT
Orobanche crenata parasitism on lentil (Lens culinaris Medik) is one of the most destructive
factors for this crop in Morocco. Field and pot assays were performed to study the mitigation
of O. crenata stress on two lentil genotypes, Bakria (partially resistant to O. crenata)
and Zaaria (susceptible), using salicylic acid (SA) and indole acetic acid (IAA). These two
hormones were applied separately at concentrations of 1 mM and 0.09 mM, respectively,
using seed pre-treatment and/or foliar spray methods. SA and IAA seed pre-treatment
for the susceptible genotype Zaaria and foliar spray for the resistant genotype Bakria gave
the best control of O. crenata under field and controlled conditions. This control reached
~91% in Zaaria and 83% in Bakria and was sometimes accompanied by an increase in plant
growth and seed yield compared to the untreated plants. Biochemical assays showed that
SA and IAA reduced O. crenata infestation in lentil through induction of systemic acquired
resistance characterized by increasing activities of phenol metabolizing enzymes (phenylalanine
ammonia-lyase, peroxidase, and polyphenol oxidase) implicated in natural defense
systems of plants. Treatment of plants with SA or IAA could be an alternative strategy of
crop protection with more satisfactory preservation of the environment.
FUNDING
This research was supported by the National Institute
of Agricultural Research of Morocco (INRA) and Ministry
of Higher Education, Scientific Research and Professional
Training of Morocco (MESRSFC) through
funding of the MEDILEG project within the European
Union 7th Framework program for research, technological
development and demonstration (ERA-Net
Project, ARIMNet).
RESPONSIBLE EDITOR
Rafał Kukawka
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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