ORIGINAL ARTICLE
In vitro evaluation of bacterial endophytes influence on Ganoderma lucidum (Leys) Karst. mycelial growth
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Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural
University, Coimbatore – 641 003, India
Corresponding author
Lingan Rajendran
Research Fellow, Department of Plant Pathology, Centre for Plant Protection Studies,
Tamil Nadu Agricultural University, Coimbatore – 641 003, Tamil Nadu, India
Journal of Plant Protection Research 2007;47(4):425-436
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ABSTRACT
Basal Stem Rot (BSR) disease caused by Ganoderma lucidum(Leys) Karst. is the most destructive disease and a major constraint in coconut production. Fifty five endophytic strains of bacteria were isolated from coconut roots of different regions. Among the isolates, EPC5 (Endophytes coconut), EPC8, EPC15, EPC29, EPC52 and Pf1 (Plant growth promoting rhizobacteria) promoted the rice seed ling growth in roll towel and pot culture method. EPC5 (Plant growth promoting endophytic bacteria), Pf1 and Trichoderma viride(Plant growth promoting fungus) effectively inhibited the G. lucidum growth in vitro. When bioagents along with farm yard manure (FYM) were heaped for different days interval the population was increased in twenty days both in sterilized and unsterilized conditions
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (31)
1.
Baker K.F., Cook R.J. 1974. Biological Control of Plant Pathogens. W.H. Freeman and Co., San Francisco, 433 pp.
2.
Baki A.A.A., Anderson J.D. 1973. Vigour determination in soybean seed by multiple criteria. Crop Sci. 31: 630–633.
3.
Bhaskaran R., Ramdoss N., Ramachandran T.K. 1988. Biological control of Thanjavur wilt disease of coconut. Indian Coconut J. 19 (6): 3–8.
4.
Bhaskaran R., Rethinam P., Nambiar K.K.N. 1989. Thanjavur wilt of coconut. J. Plant. Crops. 17: 69–79.
5.
Bhowmik B., Singh R.P., Jayaram J., Verma J.P. 2002. Population dynamics of cotton endophytic Pseudomonas, their antagonism and protective action against the major pathogens of cotton. Indian Phytopath. 55 (2): 124–132.
6.
Bruckner H., Reinecke C., Kripp T., Kieb M. 1990. Screening isolation and sequence determination of a unique group of polypeptide antibiotics from filamentous fungi. Proc. Int. Mycol. Cong.Regensburg, Federation Republic Germany, p. 224.
7.
Carrim A.J.L., Barbosa E.C., Vieira J.D.G. 2006. Enzymatic activity of endophytic bacterial isolates of Jacaranda decurrens Cham. (Carobinha-do-campo). Braz. Arch. Biol. Technol. 49 (3): 353–359.
8.
Chen C., Bauske E.M., Musson G., Rodriguez-Kabana R., Kloepper J.W. 1995. Biological control of Fusarium wilt on cotton by use of endophytic bacteria. Biol. Control 5: 83–91.
9.
Cho S.J., Lim W.J., Hong S.Y., Park S.R., Yun H.D. 2003. Endophytic colonization of balloon flower by antifungal strain Bacillus sp. CY22. Biosci. Biotechnol. Biochem. 67 (10): 2132–2138.
10.
Dennis C., Webster J. 1971. Antagonistic properties of species groups of Trichoderma 1. Production of non-volatile antibiotics. Trans. Br. Mycol. Soc. 57: 25–39.
11.
Dutta P., Das B.C. 1999. Effect of seed pelleting and soil application of Trichoderma harzianum in the management of stem rot of soybean. J. Mycol. Pl. Pathol. 29 (3): 317–322.
12.
Fukui R., Schroth M.N., Hendson M., Hancock J.G. 1994. Interaction between strains of Pseudomonads in sugar beet sphermospheres and the relationship to pericarp colonization by Pythium ultimum in soil. Phytopathology 84: 1322–1330.
13.
Garrett S.D. 1955. A century of root disease investigation. Ann. Appl. Biol. 42: 211–219.
14.
Gomez K.A., Gomez A.A. 1984. Statistical Procedure for Agricultural Research. John Wiley and Sons, New York, p. 680.
15.
Gunasekaran M., Ramdoss N., Ramiah M., Bhaskaran R., Ramanathan T. 1986.Role of neem cake in the control of Thanjavur wilt of coconut. Indian Coconut J. 17(1): 7–12.
16.
Hallmann J., Quadt-Hallmann A., Mahaffee W.F., Kloepper J.W. 1997. Bacterial endophytes in agricultural crops. Can. J. Microbiol.43: 895–914.
17.
ISTA. 1993. Proceedings of the international Seed Testing Association, International Rules for Seed Testing. Seed Sci. Technol. 21: 25–30.
18.
Karthikeyan G., Karpagavalli S., Rabindran R., Natarajan C. 2005. Biological control of basal stem rot disease in coconut. The Planter 81 (957): 777–784.
19.
Karunanithi K., Sarala L., Rabindran R., Kalaimani T., Manickam G. 2004. Effect of biocontrol agents on the management of basal stem rot (BSR) of coconut. 26th Annual Conference of ISMPP and National symposium on advances in fungal diversity and host-pathogen interactions.Goa University, October 7–9, 2004, p. 53.
20.
King E.O., Ward M.N., Raney D.E. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44: 301–307.
21.
Lattiffah Z., Harikrishna K., Tan S.G., Tan S.H., Abdullah F., Ho Y.W. 2002. Restriction analysis and sequencing of the ITS regions and 5.8 S gene of rDNA of Ganoderma isolates from infected oil palm and coconut stumps in Malaysia. Ann. Appl. Biol. 141: 133–142.
22.
Mahafee W.F., Kloepper J.W. 1997. Temporal changes in the bacterial communities of soil, rhizosphere and endorhiza associated with field-grown cucumber (Cucumis sativus L.). Microbial. Ecol. 34: 210–223.
23.
Monteiro L., Mariano R.L., Souto-Maior A.M. 2005. Antagonism of Bacillus spp. against Xanthomonas campestris pv. campestris. Braz. Arch. Biol. Technol. 48: 23–29.
24.
Rajendran L., Saravanakumar D., Raguchander T., Samiyappan R. 2006. Endophytic bacterial induction of defence enzymes against bacterial blight of cotton. Phytopathol. Mediterr. 45: 203–214.
25.
Rangasamy R. 1995. A Text Book of Agricultural Statistics. New Delhi, India: New Age International publisher Ltd, 269 pp.
26.
Rifai M.A. 1969. A revision of the genus Trichoderma. Micol. Papers 116: 1–56.
27.
Saison C., Degrange V., Oliver R., Millard P., Commeaux C., Montange D., Roux X.L. 2006. Alteration and resilience of the soil microbial community following compost amendment: effects of compost level and compost-borne microbial community. Environ. Microbiol. 8: 247–257.
28.
Sturz A.V., Matheson B.G. 1996. Populations of endophytic bacteria which influence host-resistance to Erwinia induced bacterial soft rot in potato tuber. Plant Soil 184: 265–271.
29.
Sturz A.V., Christie H.R., Matheson B.G., Arsenault W.J., Buchman N.A. 1997. Biodiversity of endophytic bacteria which colonize red clover nodules, roots, stems and foliage and their influence on host growth. Biol. Fert. Soils 25: 13–19.
30.
Thompson D.C. 1996. Evaluation of bacterial antagonist for reduction of summer patch symptoms in Kentucky blue grass. Plant Dis. 80: 856–862.
31.
Van Buren A.M., Andren C., Isbimaru C.A. 1993. Biological control of bacterial ring rot pathogen by endophytic bacteria isolated from potato. Phytopathology 83, p. 1406.