ORIGINAL ARTICLE
In vitro evaluation of antagonistic microorganisms for the control of die-back of neem causal agent Phomopsis azadirachtae
1
Department of Studies in Microbiology, Manasagangotri, University of Mysore,
Mysore – 570 006, India
2
Department of Microbiology (P.G.), Maharani’s Science College for Women,
JLB Road, Mysore – 570 005, India
3
Advisor, Labland Biodiesel Private Limited, # 98, 7
th
main, Jayalakshmipuram,
Mysore – 570 012, India
4
Department of Studies in Botany, Manasagangotri, University of Mysore,
Mysore – 570 006, India
Corresponding author
Krishna Girish
Department of Microbiology (P.G.), Maharani’s Science College for Women, JLB Road, Mysore – 570 005, India
Department of Microbiology (P.G.), Maharani’s Science College for Women, JLB Road, Mysore – 570 005, India
Journal of Plant Protection Research 2009;49(4):363-368
KEYWORDS
TOPICS
ABSTRACT
The die-back of neem caused by Phomopsis azadirachtae is a devastating disease in India reducing the life span and seed production of neem. Six isolates of antagonistic bacteria and fungi, Bacillus cereus (MTCC 430), B. subtilis (MTCC 619), Pseudomonas aeruginosa (MTCC 2581), P. oleovorans (MTCC 617), Trichoderma harzianum (MTCC 792) and T. viride (MTCC 800) were evaluated against P. azadirachtae under in vitro conditions. Culture filtrates of all these microorganisms were extracted using ethyl acetate, and the obtained fractions were tested for their antifungal activity against P. azadirachtae at different concentrations. Ethyl acetate extracts of B. subtilis and P. aeruginosa were highly effective and completely inhibited the growth of P. azadirachtae at 25 ppm concentration. Both these isolates may be considered as factors for the biological control of die-back of neem
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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