ORIGINAL ARTICLE
Identifying the key motif essential for enhancing plant defense against Botrytis cinerea by β-glucanase from Bacillus velezensis LJ02
1
Horticulture and Landscape Architecture, Tianjin Agriculture University, Tianjin, China
2
College of Engineering and Technology Architecture, Tianjin Agriculture University, Tianjin, China
3
Horticulture and Landscape Architecture, Tianjin Agriculture Univeresity, Tianjin, China
These authors had equal contribution to this work
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-02-23
Acceptance date: 2024-04-10
Online publication date: 2024-09-11
Corresponding author
Yuanhong Wang
Horticulture and Landscape Architecture, Tianjin Agriculture Univeresity, Tianjin, China
Horticulture and Landscape Architecture, Tianjin Agriculture Univeresity, Tianjin, China
Journal of Plant Protection Research 2024;64(3):253-262
HIGHLIGHTS
- β-glucanase improves the protective effect of N. benthamiana to B. cinerea
- Amino acids 5-54 in β-glucanase are necessary for the induction of plant defense responses
- qRT-PCR showed that the expression of PTI marker gene induced by N54 was significantly inhibited
KEYWORDS
TOPICS
ABSTRACT
The glycosyl hydrolase β-glucanase elicits plant immune responses against pathogens and
enhances plant immunity by activating signaling pathways. The specific functional domains
responsible for disease prevention remain unclear. In this study, transient expression of
β-glucanase significantly increased leaves resistance to Botrytis cinerea in Nicotiana benthamiana
systemic leaves. Through sequence alignment and similarity analysis, five conserved
motifs in the amino acid sequence of β-glucanase were identified, and five deletion mutants
were generated to investigate its essential regions further. Notably, the N-terminal amino
acid sites 5-54 deletion mutation of β-glucanase decreased resistance to B. cinerea infection.
These results indicate that N-terminal amino acids 5-54 (N54) are crucial for β-glucanase
induced N. benthamiana defense response and for enhancing resistance to B. cinerea. Further
analysis using real-time quantitative fluorescent PCR (qRT-PCR) revealed a significant
reduction in gene expression within the N54 region compared to that of unmutated
β-glucanase. Additionally, there was a notable reduction in the relative expression levels of
FRK, CYP71D20, WRKY7, WRKY8, ACRE31 and PTI genes. Therefore, the first 50 amino
acids at positions 5-54 within the N-terminal domain were essential for triggering plant
defense responses and enhancing resistance against B. cinerea infection. This study provides
an important theoretical foundation for systematic investigation into key functional
domains within β-glucanase that trigger defensive responses in plants against B. cinerea.
ACKNOWLEDGEMENTS
We wish to thank the Science and technology projects
of Gansu (22CX8NH226); Science and technology
projects of Gansu (23CXNH0014) for supporting this
work.
RESPONSIBLE EDITOR
Dzarifah Zulperi
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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