ORIGINAL ARTICLE
Identification of Colletotrichum spaethianum causing sugar beet leaf spot in North Dakota, USA
1
Plant Pathology, North Dakota State University, Fargo, United States
2
Molecular Plant Pathology Laboratory, USDA-ARS, Beltsville, United States
3
Environmental Microbial & Food Safety Lab, USDA-ARS, Beltsville, United States
4
Plant Pathology, Palli Siksha Bhavana, Visva Bharati University, College Street, Sriniketan, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-09-28
Acceptance date: 2024-11-25
Online publication date: 2025-07-07
HIGHLIGHTS
- Colletotrichum spaethianum causes sugar beet leaf spot in North Dakota, USA
- This is the first report of the pathogen on sugar beet in North Dakota, USA
- The report will help growers design effective management of the pathogen
KEYWORDS
TOPICS
ABSTRACT
Sugar beet leaves with dark brown to blackish, necrotic lesions were found in a grower’s
field in Casselton, North Dakota, USA in August 2021. Morphological features of the isolates
obtained in growth media from the diseased samples were observed and documented.
The pathogenicity of the randomly selected isolates developed identical disease symptoms
on the inoculated leaves. Molecular characterization of the isolates was conducted by identifying
homologies with sequences of the internal transcribed spacer, the largest subunit of
RNA polymerase II (rpb2), β-tubulin (β-tub), calmodulin (CaM), and plasma membrane
ATPase (Pma1) genes followed by multilocus phylogenic analyses. Based on morphological
characteristics, pathogenicity, and molecular analyses, the causal organism was identified
as Colletotrichum spaethianum. This is the first report of C. spaethianum causing leaf spot
on sugar beet in North Dakota, USA. The report will help growers design an effective disease
management for a novel pathogen in sugar beet in the Red River Valley of Minnesota
and North Dakota, USA.
ACKNOWLEDGEMENTS
The authors wish to thank the Sugar Beet Research and
Education Board (SBREB) of Minnesota and North
Dakota and the Department of Plant Pathology, North
Dakota State University, Fargo, ND, USA.
RESPONSIBLE EDITOR
Anna Baduro-Cieśniewska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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