ORIGINAL ARTICLE
Host plant resistance to bean common mosaic necrosis virus among snap bean cultivars in Kenya
1
Water and Agricultural Resource Management, University of Embu, Embu, Kenya
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-04-15
Acceptance date: 2023-07-20
Online publication date: 2023-10-10
Corresponding author
Journal of Plant Protection Research 2023;63(4):418-427
HIGHLIGHTS
- Snap bean cultivars and dry bean controls were screened for resistance.
- BCMNV pathotypes III and VI were identified and used for screening.
- Three dry bean cultivars were identified as potential donors of BCMNV resistance
- None of the snap bean cultivars was resistant and require improvement.
- Molecular markers SW13 and elF4E can be used for marker-assisted selection.
KEYWORDS
bean common mosaic necrosis virus (BCMNV)bean common mosaic virus (BCMV)molecular markerspathotypessnap bean
TOPICS
ABSTRACT
Snap bean production in Kenya is constrained by many pests and diseases, including the
bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV).
The occurrence of the dominant I gene in many snap bean cultivars has provided a measure
of control over BCMV but the BCMNV overcomes this resistance. The current study aimed
to screen a collection of locally grown snap bean commercial cultivars, landraces, breeding
lines, and dry bean cultivars for the expression of resistance against BCMNV under
both field and greenhouse conditions. The results showed that the evaluated snap bean
cultivars were susceptible to BCMNV. The reactions of the genotypes to BCMNV varied
from top, vein and local necrosis, mosaics, mottling, deformed leaves to stunted growth.
Positive infection was confirmed through enzyme linked immunosorbent assays. The dry
bean cultivars, which were used as resistant checks can be explored as sources of resistance
to BCMNV in future breeding programs. Molecular analysis showed that the SW13 and
elF4E markers were reliable in confirming the presence or absence of the dominant I gene
and the recessive bc-3 gene, respectively. These molecular markers are useful in markerassisted
breeding programs.
ACKNOWLEDGEMENTS
The authors are grateful to Kirkhouse Trust for financial
support, Dr. Alice Kabeja of the Rwanda Agricultural
Board and CIAT, for providing seeds of the
checks/differential cultivars and KALRO Kakamega
for providing greenhouse and laboratory facilities.
While the research project was funded by Kirkhouse
Trust, the design, execution and interpretation of the
research remains wholly the responsibility of the authors.
RESPONSIBLE EDITOR
Julia Minicka
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (25)
1.
Chilagane L.A., Tryphone G.M., Protas D., Kweka, E., Kusolwa P.M., Nchimbi-Msolla S. 2013. Incorporation of resistance to angular leaf spot and bean common mosaic necrosis virus diseases into adapted common bean (Phaseolus vulgaris L.) genotype in Tanzania. African Journal of Biotechnology 12 (27): 4343–4350. DOI: https://doi.org/10.5897/AJB201....
2.
Deligöz I., Sökmen M., Tekeoglu M. 2021. Phenotypic and molecular screening of dry bean (Phaseolus vulgaris L.) breeding lines for resistance to bean common mosaic virus and bean common mosaic necrosis virus. Acta Scientiarum Polonorum Hortorum Cultus 20 (6):7–18. DOI: https://doi.org/10.24326/asphc....
3.
Deligöz İ., Sokmen M., Kutluk Yılmaz N., Özçelik H. Tekeoğlu M. 2022. Screening of snap and dry bean (Phaseolus vulgaris L.) genotypes for resistance to Bean common mosaic virus and Bean common mosaic necrosis virus. Plant Protection Bulletin 62 (4): 5–13. DOI: https://doi.org/10.16955/bitko....
4.
Feng X., Guzmán P.J., Myers R., Karasev A.V. 2017. Resistance to Bean common mosaic necrosis virus conferred by the bc-1 gene affects the systemic spread of the virus in common bean. Phytopathology 107 (7): 93–900. DOI: https://doi.org/10.1094/PHYTO-....
5.
Hagerty C. H., Cuesta-Marcos A., Cregan P., Song Q., McClean P., Myers J. R. 2016. Mapping snap bean pod and color traits, in a dry bean× snap bean recombinant inbred population. Journal of the American Society for Horticultural Science 141 (2): 131–138. DOI: https://doi.org/10.21273/JASHS....
6.
HCDA. Horticultural Crops Development Authority. 2020 Horticulture Validation Report Nairobi, Kenya. [Available on: http://horticulture.agricultur...]. [Accessed on: 21st March 2023].
7.
Johnson W.C., Guzmán P., Mandala D., Mkandawire A.B., Temple S., Gilbertson R.L., Gepts P. 1997. Molecular tagging of the bc‐3 gene for introgression into the Andean common bean. Crop Science 37 (1): 248–254. DOI: https://doi.org/10.2135/cropsc....
8.
Kamiri A.K., Arunga E.E., Rotich F., Otsyula R. 2021. Response of French bean genotypes to Colletotrichum lindemuthianum and evaluation of their resistance using SCAR markers. African Journal of Biotechnology 20 (2): 51–65. DOI: http://doi: 10.5897/AJB2020.17279.
9.
Kelly J.D., Gepts P. Miklas P.N., Coyne D. 2003. Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea. Field Crops Research 82 (2–3): 135–154. DOI: https://doi.org/10.1016/S0378-....
10.
Kilic H.C., Hesna K.O., Yardimci N. 2020. Bean common mosaic virus and bean common mosaic necrosis virus infections in bean production areas in The Lakes Region of Turkey. Avrupa Bilim ve Teknoloji Dergisi 19: 386–392. DOI: https://doi.org/10.31590/ejosa....
11.
Larsen R.C., Druffel K.L., Wyatt S.D. 2011. The complete nucleotide sequences of bean common mosaic necrosis virus strain NL-5, NL-8 and TN-1. Archives of Virology 156 (4): 729–732. DOI: https://doi.org/10.1007/s00705....
12.
Mahuku G.S. 2004. A simple extraction method suitable for PCR-based analysis of plant, fungal, and bacterial DNA. Plant Molecular Biology Reporter 22 (1): 71–81. DOI: https://doi.org/10.1007/BF0277....
13.
Mangeni B.C., Abang M., Omuse C.N., Leitich R., Arinaitwe W., Mukoye B., Kelly J., Were H. 2014. Distribution and pathogenic characterization of bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) in western Kenya. Journal of Agri-Food and Applied Sciences 2 (10): 308–316.
14.
Mangeni B.C., Were H.K., Ndong'a M., Mukoye B. 2020. Incidence and severity of bean common mosaic disease and resistance of popular bean cultivars to the disease in western Kenya. Journal of Phytopathology 168 (9): 501–515. DOI: https://doi.org/10.1111/jph.12....
15.
Melotto M., Afanador L., Kelly. J.D. 1996. Development of a SCAR marker linked to the I gene in common bean. Genome 39 (6): 1216–1219. DOI: https://doi.org/10.1139/g96-15....
16.
Mutuku J.M, Wamonje F.O., Mukeshimana G., Njuguna J., Wamalwa M., Choi S., Tungadi T., Djikeng A., Carr J.P., Harvey J. 2018. Metagenomic analysis of plant virus occurrence in common bean (Phaseolus vulgaris) in Central Kenya. Frontiers in Microbiology 9: 2939. DOI: https://doi.org/10.3389/fmicb.....
17.
Muute N., Muli A.B., Charles O. 2021. Evaluation of bean common mosaic disease and associated aphid vector, Aphis fabae L., on common bean (Phaseolus vulgaris L.) production in lower eastern Kenya. International Journal of Pathogen Research 8 (3):1–18. DOI: http:// doi: 10.9734/IJPR/2021/v8i330203.
18.
Mwaipopo B., Nchimbi-Msolla S., Njau P.J., Mark D., Mbanzibwa D.R. 2018. Comprehensive surveys of bean common mosaic virus and bean common mosaic necrosis virus and molecular evidence for occurrence of other Phaseolus vulgaris L. viruses in Tanzania. Plant Disease 102 (11): 2361–2370. DOI: https://doi.org/10.1094/PDIS-0....
19.
Naderpour M., Lund O.S., Larsen R., Johansen E. 2010. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc‐3 is associated with the homozygotic presence of a mutated eIF4E allele. Molecular Plant Pathology 11 (2): 255–263. DOI: https://doi.org/10.1111/j.1364....
20.
Okii D., Mukankusi C., Sebuliba S., Tukamuhabwa P., Tusiime G., Talwana H., Ogong T., Namayanja A. Paparu P., Gepts P. 2018. Genetic variation, Heritability estimates and G × E effects on yield traits of Mesoamerican common bean (Phaseolus vulgaris L) germplasm in Uganda. Plant Genetic Resources 16 (3): 237–248. DOI: 10.1017/S1479262117000259.
21.
Pasev G., Kostova D., Sofkova S. 2014. Identification of genes for resistance to Bean common mosaic virus and Bean common mosaic necrosis virus in snap bean (Phaseolus vulgaris L.) breeding lines using conventional and molecular methods. Journal of Phytopathology 162 (1): 19–25. DOI: https://doi: 10.1111/jph.12149.
22.
Silbernagel M.J., Mink G.I., Zhao R.L., Zheng G.Y. 2001. Phenotypic recombination between bean common mosaic and bean common mosaic necrosis potyviruses in vivo. Archives of Virology 146: 1007–1020. DOI: https://doi.org/10.1007/s00705....
23.
Soler-Garzon A, McClean P. E., Miklas P.N. 2021. Coding mutations in vacuolar protein-sorting 4 AAA+ ATPase endosomal sorting complexes required for transport protein homologs underlie bc-2 and new bc-4 gene conferring resistance to Bean common mosaic virus in common bean. Frontiers in Plant Science 12: 769247. DOI: https://doi.org/10.3389/fpls.2....
24.
Tang M., Feng X. 2023. Bean common mosaic disease: etiology, resistance resource, and future prospects. Agronomy 13 (1): 58. DOI: https://doi.org/10.3390/agrono....
25.
Worrall E.A., Wamonje F.O., Mukeshimana G., Harvey J.J., Carr J.P., Mitter N. 2015. Bean common mosaic virus and Bean common mosaic necrosis virus relationships, biology, and prospects for control. p. 1–46. In: "Advances in Virus Research" (M. Kielian, K. Maramorosch, T. Mettenleiter, eds.). Academic Press, Burlington. DOI: https://doi.org/10.1016/bs.aiv....
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| ISSN: | 1427-4345 |
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