ORIGINAL ARTICLE
Genetic identification of an invasive plant (Parthenium hysterophorus) and its crude extract phytotoxicity against palm oil weed (Diodia ocimifolia)
More details
Hide details
1
Department of Chemical Technology and Food, Politeknik Tun Syed Nasir Syed Ismail, Johor, Malaysia
2
Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
3
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia
4
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-01-13
Acceptance date: 2022-03-30
Online publication date: 2022-06-29
Corresponding author
Norazlinaliza Salim
Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, UPM, 43400, Serdang, Malaysia
Journal of Plant Protection Research 2022;62(2):223-229
HIGHLIGHTS
- The local plant namely Rumput Miang Mexico was genetically identified as Parthenium hysterophorus by using DNA profiling technique.
- P.hysterophorus crude extract (PHCE) has significantly inhibited the germination of weed in Malaysian palm oil plantations.
- Overall, the seed germination showed an effect of hormesis where PHCE acted as a stimulant at a low dose, and inhibit as increasing the concentration.
KEYWORDS
TOPICS
ABSTRACT
This paper reports a study on genetic identification of Parthenium hysterophorus and the
ability of P. hysterophorus crude extract (PHCE) to inhibit germination of weeds in palm oil
plantations, particularly slender buttonweed (Diodia ocimifolia). Parthenium hysterophorus
is a noxious plant with invasive properties. It has become one of the world’s seven most
devastating and hazardous weed‑s due to its allelopathic properties. However, a comprehensive
study on its genetic identification and its herbicidal activity against weeds in palm
oil plantations had never been carried out. In the present study, internal transcribes spacer
(ITS) region-based analysis was used as a molecular
marker to ascertain the local Rumput
Miang Mexico (RMM) as a P. hysterophorus species. From the results, the DNA sequence
obtained was 99% matched with P. hysterophorus species database. In the dose-response
bioassay study, the phytotoxicity of PHCE on D. ocimifolia was simulated under laboratory
conditions. Methanolic extract of P. hysterophorus significantly inhibited germination and
growth of shoots and radicles of D. ocimifolia at low concentrations (1−5 g ∙ l−1). A study on
the herbicidal activity of PHCE could be an additional management approach for this phytotoxic
species and an alternative way to naturally control weeds on palm oil plantations.
ACKNOWLEDGEMENTS
The author would like to thank the Ministry of Higher
Education Malaysia for my Ph.D. scholarship for
Zainuddin N.J. and the Department of Agriculture
Malaysia for giving permission and assistance during
the collection of P. hysterophorus plant.
RESPONSIBLE EDITOR
Łukasz Sobiech
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (37)
1.
Adkins S., Shabbir A. 2014. Biology, ecology and management of the invasive parthenium weed (Parthenium hysterophorus L.). Pest Management Science 70: 1023–1029. DOI:
https://doi.org/10.1002/ps.370....
2.
Bajwa A.A., Weston P.A., Gurusinghe S., Latif S., Adkins S.W., Weston L.A. 2020. Toxic potential and metabolic profiling of two Australian biotypes of the invasive plant Parthenium. Toxins 12: 47. DOI:
https://doi.org/https://doi.or....
3.
Baldwin B.G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the compositae. Molecular Phylogenetics and Evolution 1: 3–16. DOI:
https://doi.org/10.1016/1055-7....
4.
Belz R.G., Cedergreen N. 2010. Parthenin hormesis in plants depends on growth conditions. Environmental and Experimental Botany 69: 293–301. DOI:
https://doi.org/10.1016/j.enve....
5.
Cedergreen N., Streibig J.C., Kudsk P., Mathiassen S.K., Duke S.O. 2007. The occurrence of hormesis in plants and algae. Dose-Response: A Publication of International Hormesis Society 5: 150–162. DOI:
https://doi.org/10.2203/dose-r....
6.
Chen S., Yao H., Han J., Liu C., Song J., Shi L., Zhu Y., Ma X., Gao T., Leon C. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS ONE 5: 1–8. DOI:
https://doi.org/10.1371/journa....
7.
De Castro R.D., Van Lammeren A.A. M., Groot S.P.C., Bino R.J., Hilhorst H.W.M. 2000. Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not. Plant Physiology 122: 327–335. DOI:
https://doi.org/10.1104/pp.122....
8.
Dilipkumar M., Chuah T.S., Goh S.S., Sahid I. 2020. Weed management issues, challenges, and opportunities in Malaysia. Crop Protection 134. DOI:
https://doi.org/10.1016/j.crop....
9.
Hazrati H., Saharkhiz M.J., Niakousari M., Moein M. 2017. Natural herbicide activity of Satureja hortensis L. essential oil nanoemulsion on the seed germination and morphophysiological features of two important weed species. Ecotoxicology and Environmental Safety 142: 423–430. DOI:
https://doi.org/10.1016/j.ecoe....
10.
Javaid A., Shafique S., Shafique S. 2011. Management of Parthenium hysterophorus (Asteraceae) by Withania somnifera (Solanaceae). Natural Product Research 25: 407–416. DOI:
https://doi.org/10.1080/147864....
11.
Kader M.A. 2005. A comparison of seed germination calculation formulae and the associated interpretation of resulting data. Journal and Proceeding of the Royal Society of New South Wales 138: 65–75.
12.
Khan N., O’Donnell C., George D., Adkins S.W. 2013. Suppressive ability of selected fodder plants on the growth of Parthenium hysterophorus. Weed Research 53: 61–68. DOI:
https://doi.org/10.1111/j.1365....
13.
Khan Naeem Shabbir A., George D., Hassan G., Adkins S.W. 2014. Suppressive fodder plants as part of an integrated management program for Parthenium hysterophorus L. Field Crops Research 156: 172–179. DOI:
https://doi.org/10.1016/j.fcr.....
14.
Kumar G., Gautam N. 2008. Allelotoxicity of Parthenium leaf extracts on cytomorphological behaviour of sunflower (Helianthus annuus). Journal of Environmental Biology 29: 243–247. DOI:
http://www.ncbi.nlm.nih.gov/pu....
15.
Lim C.J., Basri M., Omar D., Abdul Rahman M.B., Salleh A.B., Raja Abdul Rahman R.N.Z. 2013. Green nanoemulsionladen glyphosate isopropylamine formulation in suppressing creeping foxglove (A. gangetica), slender button weed (D. ocimifolia) and buffalo grass (P. conjugatum). Pest Management Science 69: 104–111. DOI:
https://doi.org/10.1002/ps.337....
16.
Lim C.J., Basri M., Ee G.C.L, Omar D. 2017. Phytoinhibitory activities and extraction optimization of potent invasive plants as eco-friendly weed suppressant against Echinochloa colona (L.) Link. Industrial Crops and Products 100: 19–34. DOI:
https://doi.org/10.1016/j.indc....
17.
Marwat K.B., Khan M.A., Nawaz A., Amin A. 2008. Parthenium hysterophorus L. A Potential source of bioherbicide. Pakistan Journal of Botany 40: 1933–1942.
18.
Msafiri C.J., Tarimo M.T., Ndakidemi P.A. 2013. Allelopathic effects of Parthenium hysterophorus on seed germination, seedling growth, fresh and dry mass production of Alysicurpus glumaceae and Chloris gayana. American Journal of Research Communication 1: 190–205.
19.
Munzuroglu O., Geckil H. 2002. Effects of metals on seed germination, root elongation, and coleoptile and hypocotyl growth in Triticum aestivum and Cucumis sativus. Archives of Environmental Contamination and Toxicology 43: 203–213. DOI:
https://doi.org/10.1007/s00244....
20.
Netsere A., Mendesil E. 2011. Allelopathic effects of Parthenium hysterophorus L. aqueous extracts on soybean (Glycine max L.) and haricot bean (Phaseolus vulgaris L.) seed germination, shoot and root growth and dry matter production. Journal of Applied Botany and Food Quality 84: 219–222.
21.
Ojija F., Arnold S.E.J., Treydte A.C. 2019. Bio-herbicide potential of naturalised Desmodium uncinatum crude leaf extract against the invasive plant species Parthenium hysterophorus. Biol Invasions 21: 3641–3653. DOI:
https://doi.org/10.1007/.s1053....
22.
Ojija F., Arnold S.E., Treydte A.C. 2021. Plant competition as an ecosystem-based management tool for suppressing Parthenium hysterophorus in rangelands. Rangelands 43 (2): 57−64. DOI:
https://doi.org/10.1016/j.rala....
23.
Ojija F., Ngimba C. 2021. Suppressive abilities of legume fodder plants against the invasive weed Parthenium hysterophorus (Asteraceae). Environmental and Sustainability Indicators 10: 100111. DOI:
https://doi.org/10.1016/j.indi....
25.
Poulin R., Presswell B. 2016. Taxonomic quality of species descriptions varies over time and with the number of authors, but unevenly among parasitic taxa. Systematic Biology 65: 1107–1116. DOI:
https://doi.org/10.1093/sysbio....
26.
Reddy D.M., Qazi N.A., Sawant S.D., Bandey A.H., Srinivas J., Shankar M., Sampath Kumar H.M. 2011. Design and synthesis of spiro derivatives of parthenin as novel anti-cancer agents. European Journal of Medicinal Chemistry 46: 3210–3217. DOI:
https://doi.org/10.1016/j.ejme....
27.
Rutherford M., Flood J., Sastroutomo S.S. 2011. Roundtable for Sustainable Palm Oil (RSPO): Research Project on Integrated Weed Management Strategies for Oil Palm. Available on:
https://www.rspo.org/file/RSPO IWM_FINAL REPORT to RSPO 9 5 11.pdf [Accessed: 27 June 2018].
28.
Sameem R. 2018. Application of PCR for the detection of universal fungi in infant food products sold in Sri Lanka. International Journal of Scientific and Research Publication 8: 527–542. DOI:
https://doi.org/10.29322/IJSRP....
29.
Singh H.P., Batish D.R., Kohli R.K., Saxena D.B., Arora V. 2002. Effect of parthenin − a sesquiterpene lactone from Parthenium hysterophorus – on early growth and physiology of Ageratum conyzoides. Journal of Chemical Ecology 28: 2169–2179. DOI:
https://doi.org/10.1023/A:1021....
30.
Soltys D., Krasuska U., Bogatek R., Gniazdowska A. 2013. Allelochemicals as bioherbicides — present and perspectives. In: “Herbicides-Current Research and Case Studies in Use”. (J.P. Andrew, A.K Jessica, eds). IntechOpen. London, United Kingdom. DOI: 10.5772/56185.
31.
Sun Y., Skinner D.Z., Liang G.H., Hulbert S.H. 1994. Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA. Theoretical Applied Genetics 89: 26–32. DOI:
https://doi.org/10.1007/BF0022....
32.
Tan M., Liao F., Hou L., Wang J., Wei L., Jian H., Xu X., Li J., Liu L. 2017. Genome-wide association analysis of seed germination percentage and germination index in Brassica napus L. under salt and drought stresses. Euphytica 213: 1–15. DOI:
https://doi.org/10.1007/s10681....
33.
Tefera T. 2002. Allelopathic effects of Parthenium hysterophorus extracts on seed germination and seedling growth of Eragrostis tef. Journal of Agronomy and Crop Science 188: 306–310.
34.
Tobe K., Zhang L., Omasa K. 2005. Seed germination and seedling emergence of three annuals growing on desert sand dunes in China. Annals of Botany 95: 649−659.
35.
Wagner R., Kogan M., Parada A.M. 2003. Phytotoxic activity of root absorbed glyphosate in corn seedlings (Zea mays L.). Weed Biology and Management 3: 228–232. DOI:
https://doi.org/10.1046/j.1444....
36.
Woittiez L.S., Van Wijk M.T., Slingerland M., Van Noordwijk M., Giller K.E. 2017. Yield gaps in oil palm: A quantitative review of contributing factors. European Journal of Agronomy 83: 57–77. DOI:
https://doi.org/10.1016/j.eja.....
37.
Won H., Renner S.S. 2005. The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum. Molecular Phylogenetics and Evolution 36: 581–597. DOI:
https://doi.org/10.1016/j.ympe....