ORIGINAL ARTICLE
Genetic diversity of Xanthomonas citri subsp. citri, causal agent of citrus canker
1
Department of Plant Protection, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2
Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Submission date: 2013-04-13
Acceptance date: 2013-09-26
Corresponding author
Farimah Arshadi
Department of Plant Protection, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Department of Plant Protection, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Journal of Plant Protection Research 2013;53(4):312-316
KEYWORDS
TOPICS
ABSTRACT
A total of 25 samples of canker disease from different part of West Malaysia were isolated from three different hosts. After various diagnostic tests, the samples were identified as Xanthomonas citri subsp. citri (Xcc), and were also pathogenic to four tested citrus species. Molecular characterization using rep-PCR fingerprinting was carried out on the isolates. Cluster analysis using the combined banding patterns of ERIC and BOX-PCR clearly divided the isolates into different clusters according to their geographical origin, but not to their host species. A relatively high amount of genetic diversity was observed among isolates, as a group of isolates from a more restricted part of Malaysia separated from the rest with relatively low similarity, indicating that there might be distinct pathotypes of the bacterium present in Malaysia.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (14)
1.
Civerolo E.L. 1984. Bacterial canker disease of citrus. J. Rio Grande Valley Hort. Soc. 37 (8): 127–146.
2.
Cuberto J., Graham J.H. 2002. Genetic relationship among worldwide strains of Xanthomonas causing canker in citrus species and design of new primers. Appl. Environ. Microbiol. 68 (3): 1257–1264.
3.
Dice L.R. 1945. Measures of the amount of ecological association between species. Ecology 26 (3): 297–302.
4.
Gottwald T.R., Graham J.H., Schubert T.S. 2002. Citrus canker: the pathogen and its impact. Plant Health Prog. DOI: 10.1094/PHP-2002-0812-01-RV. http://www.plantmanagementwork...: March 12, 2010].
5.
Graham J.H., Hartung J.S., Chase A.R. 1990. Pathological, restriction fragment length polymorphism and fatty acid profile relationships between Xanthomonas campestris from citrus and noncitrus hosts. Phytopathology 80 (6): 826–836.
6.
Hartung J.S., Civerolo E.L. 1989. Restriction fragment length polymorphisms distinguish Xanthomonas campestris strains isolated from Florida citrus nurseries from Xanthomonas campestris pv. citri . p. 503–508. Proc. 7th Int. Conf. Plant Pathogenic Bacteria. Akademiai Kiado, Budapest, Hungary.
7.
Hartung J.S., Daniel J.F., Pruvost O.P. 1993. Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method. Appl. Environ. Microbiol. 59 (4): 1143–1148.
8.
Khodakaramian G., Swings J. 2002. AFLP fingerprinting of the strains of Xanthomonas axonopodis inducing citrus canker disease in southern Iran. J. Phytopathol. 150 (4–5): 227–231.
9.
Lee Y.H., Lee S., Lee D.H., Ji S.H., Chang H.Y., Heu S., Hyun J.W., Ra D.S., Park E.W. 2007. Differentiation of citrus bacterial canker strains in Korea by host range, rep-PCR fingerprinting and rDNA analysis. Eur. J. Plant Pathol. 121 (1): 97–102.
10.
Lin H.C., Hsu S.T., Hwang A.S., Tzeng K.C. 2005. Phenotypic and genetic characterization of novel strains of Xanthomonas axonopodis p v. citri which induce atypical symptoms on citrus leaves in Taiwan. Plant Pathol. Bull. 14: 227–238.
11.
Mahuku G.S. 2004. A simple extraction method suitable for PCR based analysis of plant, fungal, and bacterial DNA. Plant Mol. Biol. Rep. 22 (1): 1–11.
12.
Trindade L.C., Lima M.F., Ferreira M.A.S.V. 2005. Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting. Fitopatologia Brasileira 30 (1): 46–54.
13.
Vernière C., Hartung J.S., Pruvost O.P., Civerolo E.L., Alvarez A.M., Maestri P., Luisetti J. 1998. Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. citri from southwest Asia. Eur. J. Plant Pathol. 104 (5): 477–487.
14.
Yap I.V., Nelson R.J. 1996. Winboot: A program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrogram. IRRI Res. Pap. Ser. No. 14. International Rice Research Institute, Manila, Philippines, 22 pp.
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
