ORIGINAL ARTICLE
First report of Pythium aphanidermatum infecting tomato in Egypt and controlling it using biogenic silver nanoparticles
1
Plant Pathology, National Research Centre, National Research Centre, Dokki, Giza, Egypt.
2
Agricultural Microbiology Department, National Research Centre, 33 El-Buhouth St., (Former El-Tahrir) 12622, Dokki, Giza, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2017-12-03
Acceptance date: 2018-01-15
Corresponding author
Ibrahim Elshahawy
Plant Pathology, National Research Centre, National Research Centre, 33 El-Buhouth St., (Former El-Tahrir) 12622, Dokki, Giza, Egypt., National Research Centre, Giza, Egypt
Plant Pathology, National Research Centre, National Research Centre, 33 El-Buhouth St., (Former El-Tahrir) 12622, Dokki, Giza, Egypt., National Research Centre, Giza, Egypt
Journal of Plant Protection Research 2018;58(2):137-151
KEYWORDS
TOPICS
ABSTRACT
A number of 16 fungal isolates were recovered from tomato plants displaying symptoms of wilting, dead plant, root rot with crown and stem rot. These isolates were classified as belonging to 6 species, namely: Alternaria solani, Chaetomium globosum, Fusarium solani, Fusarium oxysporum, Pythium sp. and Rhizoctonia solani. Isolates of Pythium were prevalence and demonstrated to be high pathogenic than the other fungal isolates. It causes damping-off, root rot, sudden death, stem rot and fruit rot. The pathogen was identified as Pythium aphanidermatum based on morphological, cultural, and molecular characteristics. Biogenic silver nanoparticles were produced using Fusarium oxysporum strain and characterized by TEM microscopy. The size of these particles was ranged between 10-30 nm and the shape was spherical. In vitro, biogenic silver nanoparticles (AgNPs) showed antifungal activity against P. aphanidermatum. In greenhouse and field experiments, AgNPs treatment significantly reduced the incidence of sudden death of tomato plants due to root rot produced by P. aphanidermatum compared to the control. All of the assayed treatments were effective and the treatment of root dipping plus soil drenching was the most effective one. To the best of our knowledge, this report describes the first time that P. aphanidermatum was observed on tomato in Egypt, and the possibility of its control with AgNPs.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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