ORIGINAL ARTICLE
First record of a novel begomovirus and satellites associated with leaf curl disease of passion fruit from India
1
Central Horticultural Experimental Station, Indian Council of Agricultural Research – Indian Institute of Horticultural Research, Chettalli, Madikeri, Karnataka, India
2
Department of Plant Pathology, University of Agricultural Sciences, Gandhi Krishi Vigyana Kendra, Bangalore, Karnataka, India
3
Indian Council of Agricultural Research – Directorate of Cashew Research, Puttur, Karnataka, India
4
Department of Forest Biology and Tree Improvement, University of Agricultural Science, Dharwad, Sirsi, Karnataka, India
5
Division of Plant Pathology, Indian Council of Agricultural Research – Indian Agricultural Research Institute, New Delhi, India
6
Indian Council of Agricultural Research – Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore, Karnataka, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2021-08-11
Acceptance date: 2021-11-19
Online publication date: 2022-03-17
Corresponding author
Venkataravanappa Venkataravanappa
Central Horticultural Experimental Station, Indian Council of Agricultural Research – Indian Institute of Horticultural Research, Chettalli, Madikeri, Karnataka, India
Central Horticultural Experimental Station, Indian Council of Agricultural Research – Indian Institute of Horticultural Research, Chettalli, Madikeri, Karnataka, India
Journal of Plant Protection Research 2022;62(1):78-92
KEYWORDS
TOPICS
ABSTRACT
Passion fruit is an important fruit crop grown in parts of southern and north-eastern states
of India. Leaf curl symptoms typical to begomovirus infection were observed on passion
fruit plants at three locations of Madikeri District, Karnataka State, India. The disease incidence
ranged from 10–20% in all the locations. In order to determine if the begomovirus
was associated with leaf curl disease of passion fruit, 20 infected samples collected from
different locations were subjected to PCR analysis using primers specific to begomovirus.
This resulted in an expected PCR product of ~1.2 kb. Sequence analysis of these products
revealed that they have more than 98% similarity among them and have similarity with
other begomoviruses. Complete genome sequencing of begomovirus associated with one
sample (PF1 collected from CHES, Madikeri) was done using RCA. Further, sequencing
of betasatellite and alphasatellite was done after PCR amplification using specific
primers. Complete DNA-A sequence of PF-isolate with other begomoviruses revealed that
it shared nucleotide (nt) identity of 87.8 to 88.8% with Ageratum enation virus. This indicated
the association of a novel begomovirus with leaf curl disease of passion fruit in
India, for which we propose the name, Passion fruit leaf curl virus (PFLCuV) [IN-Kar-18].
PFLCuV associated betasatellite shared 98.3% sequence identity with Tomato leaf curl
Bangladesh betasatellite, while alphasatellite had 95.7% sequence identity with Cotton leaf
curl Multan alphasatellite. Recombinant analysis indicated a major component of PFLCuV
DNA-A may have originated from a recombination of earlier reported begomoviruses. Recombination
as well as GC plot analysis showed that the recombination occurred in the
genome regions having low GC content regions of PFLCuV. However, there is no evidence
of recombination in alphasatellite and betasatellite associated with leaf curl disease of passion
fruit. This is the first record of a novel begomovirus and satellites associated with leaf
curl disease of passion fruit from India.
ACKNOWLEDGEMENTS
The authors are grateful to the Director, Indian Institute
of Horticultural Research, Bangalore for providing
research facilities.
RESPONSIBLE EDITOR
Julia Minicka
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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