ORIGINAL ARTICLE
Field survey of Fusarium stem rot of lisianthus (Eustoma grandiflorum) cultivated in Okinawa, Japan
1
Okinawa Agricultural Research Center, Okinawa, Japan
2
TechnoSuruga Laboratory Co. Ltd, Shizuoka, Japan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-02-02
Acceptance date: 2023-04-18
Online publication date: 2023-09-04
Journal of Plant Protection Research 2023;63(3):271-279
HIGHLIGHTS
- The pathogen of Fusarium stem rot (Kukigusare-byo) was identified as Fusarium avenaceum based on multigene sequences analyses. Indeed, the pathogen is clustered separately from the other F. avenaceum strains isolated from lisianthus in USA. The diseased lisianthus plants appeared from November to January. The ratio of farms where the disease occurred against the total number of farms was 43.8% in Okinawa Main Island.
KEYWORDS
TOPICS
ABSTRACT
Lisianthus (Eustoma grandiflorum) has become a major flowering plant in Okinawa, the
southernmost prefecture of Japan. Its cultivation area has increased steadily with each
passing year for two decades. Simultaneously, many types of lisianthus diseases related to
damping-off symptoms have also increased dramatically. To create a strategy for preventing
the disease, disease symptoms and pathogenic organisms of primary problematic disease
with seasonal variation in the emergence were investigated. The symptoms were diagnosed
as Fusarium stem rot (Kukigusare-byo) and the pathogen of the disease was identified as
Fusarium avenaceum based on multigene sequences analyses. Indeed, the PCR result of
the isolated strain in this study was the same as that isolated from lisianthus plants with
Fusarium stem rot in Hokkaido Prefecture. Furthermore, the pathogen is clustered separately
from the other F. avenaceum strains isolated from lisianthus in the USA. Diseased
lisianthus plants spread throughout greenhouses even though several fungicides were applied.
Additionally, they appeared from November to January and increased to 0.3% of the
total number. Fusarium stem rot was found in 43.8% of the total number of farms from
2020–2021 in Okinawa Main Island.
ACKNOWLEDGEMENTS
We would like to thank all the farmers for their cooperation
with our investigation. We are grateful to Mrs.
Oyama, supervisory technician, for her support with
the investigation. We appreciate the technical support
of DNA sequence by Dr. Tarora. We also would like to thank Mr. Takushi and Dr. Kawano for their technical advice.
RESPONSIBLE EDITOR
Ewa Moliszewska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (25)
1.
Arshi J., Shabbir A. 2020. Utilization of chemical fungicides in managing the wilt disease of chickpea caused by Fusarium oxysporum f. sp. cicero. Archives of Phytopathology and Plant Protection 53 (17–18): 876–898. DOI: 10.1080/03235408.2020.1803705.
2.
Bradbury J.F. 1970. Isolation and preliminary study of bacteria from plants. Pest Articles and News Summaries 16: 632–637.
3.
Broders K.D., Lipps P.E., Paul P.A., Dorrance A.E. 2007. Evaluation of Fusarium graminearum associated with corn and soybean seed and seedling disease in Ohio. Plant Disease 91 (9): 1155–1160. DOI: 10.1094/PDIS-91-9-1155.
4.
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.
5.
Harukuni H., Fujio K. 2016. Fusarium diseases on ornamental plants occurred in Hokkaido (1) Lisianthus stem rot. Research Bulletin of the Hokkaido National Agricultural Experiment Station 100: 15–21. Available on: https://www.hro.or.jp/list/agr... (in Japanese, with English Summary).
6.
Horita H., Kodama F. 1995. Fusarium avenaceum, the causal agent of lisianthus stem rot. Japanese Journal of Phytopathology 61 (6): 646. DOI: https://doi.org/10.3186/jjphyt....
7.
Iwata Y. 1991. The wilt symptom of lisianthus by Fusarium avenaceum and Fusarium oxysporum. Japanese Journal of Phytopathology 57 (1): 123. DOI: https://doi.org/10.3186/jjphyt....
8.
Laraba I., Busman M., Geiser D.M., O'Donnell K. 2022. Phylogenetic diversity and mycotoxin potential of emergent phytopathogens within the Fusarium tricinctum species complex. Phytopathology 112: 1284–1298. DOI: https://doi.org/10.1094/PHYTO-....
9.
Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120. DOI: 10.1007/BF01731581.
10.
Kumar S., Stecher G., Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0. for bigger datasets. Molecular Biology and Evolution 33: 1870–1874.
11.
Leslie J.F., Summerell B.A. 2006. The Fusarium laboratory manual. Blackwell Publishing, Oxford Chapter 13: 132–133.
12.
Liu Y.J., Whelen S., Hall B.D. 1999. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16: 1799–1808.
13.
Nalim F.A., Elmer W.H., McGovern R.J., Geiser D.M. 2009. Multilocus phylogenetic diversity of Fusarium avenaceum pathogenic on lisianthus. Phytopathology 99: 462–468. DOI: 10.1094/PHYTO-99-4-0462.
14.
O’Donnell K. 1993. Fusarium and its near relatives. p. 225–233. In: “The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics” (D.R. Reynolds, J. Taylor, eds). CABI, Wallingford, 350 pp.
15.
O'Donnell K., Kistler H.C., Cigelnik E., Ploetz R.C. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences USA 95: 2044–2049. DOI: 10.1073/pnas.95.5.2044.
16.
O'Donnell K., Sutton D.A., Rinaldi M.G., Sarver B.A., Balajee S.A., Schroers H.J., Summerbell R.C., Robert V.A., Crous P.W., Zhang N., Aoki T., Jung K., Park J., Lee Y.H., Kang S., Park B., Geiser D.M. 2010. Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. Journal of Clinical Microbiology 48: 3708–3718. DOI: 10.1128/JCM.00989-10.
17.
Reeb V., Lutzoni F., Roux C. 2004. Contribution of RPB2 to multilocus phylogenetic studies of the euascomycetes (Pezizomycotina, Fungi) with special emphasis on the lichen-forming Acarosporaceae and evolution of polyspory. Molecular Phylogenetics and Evolution 32: 1036–1060. DOI: 10.1016/j.ympev.2004.04.012.
18.
Saitou N., Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4 (4): 406–425. DOI: https://doi.org/10.1093/oxford....
19.
Senatore M.T., Ward T.J., Cappelletti E., Beccari G., McCormick S.P., Busman M., Laraba I., O'Donnell K., Prodi A. 2021. Species diversity and mycotoxin production by members of the Fusarium tricinctum species complex associated with Fusarium head blight of wheat and barley in Italy. International Journal of Food Microbiology 358: 109298. DOI: https://doi.org/10.1016/j.ijfo....
20.
The Phytopathological Society of Japan. 2022. Common Names of Plant Diseases in Japan (2022.8 edition). The Phytopathological Society of Japan, Tokyo.
21.
Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673–4680. DOI: https://doi.org/10.1093/nar/22....
22.
Tomita T., Ogawara T., Ichimura T., Nagatsuka H. 2005. Control of soil diseases and nematode for Russell prairie gentian by soil solarization and hot water injection in greenhouse. Bulletin of the Horticultural Institute, Ibaraki Agricultural Center 13: 43–47.
23.
Turner A.S., Lees A.K., Rezanoor H.N., Nicholson P. 1998. Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathology 47: 278–288. DOI:.
24.
White T.J., Bruns T., Lee S., Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. p. 315–322. In: “PCR Protocols: A Guide to Methods and Applications” (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, eds). Academic Press, San Diego, 482 pp.
25.
Yli-Mattila T., Abbas A., Gavrilova O., Gagkaeva T. 2022. Molecular variation and phylogeny within Fusarium avenaceum and related species. Diversity 14: 574. DOI: https://doi.org/10.3390/d14070....
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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