ORIGINAL ARTICLE
Evaluation of the antagonistic potential of Trichoderma spp. against Fusarium oxysporum F.28.1A
1
Faculty of Crop Science, College of Agriculture, Can Tho University, Viet Nam
2
Biotechnology Research and Development Institute, Can Tho University, Viet Nam
3
Experimental and Practical Area, An Giang University – Vietnam National University, Ho Chi Minh City, Viet Nam
4
Tropical Crop Science Laboratory, Faculty of Agriculture, Kagoshima University, Japan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-05-27
Acceptance date: 2022-10-10
Online publication date: 2023-02-17
Corresponding author
Journal of Plant Protection Research 2023;63(1):13-26
HIGHLIGHTS
- The isolated strains Trichoderma were able to produce enzymes including chitinase, exo-β-1,3-glucanase, and endo-β-1,3-glucanase at levels of 0.34–0.44, 0.017–0.034, and 0.032–0.121 UI mL-1, respectively.
- The experimental results revealed that supplementing with a mixture of the 5 isolated strains of Trichoderma reduced the disease’s prevalence by 35%.
KEYWORDS
TOPICS
ABSTRACT
The objective of this study was to evaluate the antagonistic activity of Trichoderma spp.
against wild pathogen Fusarium oxysporum F.28.1A, which causes wilt disease on sesame.
Twenty-six isolates of Trichoderma spp. isolated from soil samples were tested to control
F. oxysporum F.28.1A. Prescreening showed that five isolates were T-02B1, T-18B2, T-20B1,
T-28B1, and T-29A1, based on the lowest values of colony radius of F. oxysporum F.28.1A.
The selected isolates were identified by their ITS region as T. yunnanense T-02B1, T. lentiforme
T-18B2, T. asperellum T-20B1, T. hamatum T-28B1, and T. hamatum T-29A1,
with similarities around 96–100%. The isolates selected were able to produce enzymes including
chitinase, exo-β-1,3-glucanase, and endo-β-1,3-glucanase at levels of 0.34–0.44,
0.017–0.034, and 0.032–0.121 UI · ml–1, respectively, which were considered to be a mechanism
to prevent the growth of F. oxysporum F.28.1A. The isolates tested were applied in soil
pots to prevent damage from F. oxysporum F.28.1A as a following experiment. The greenhouse
experiment was arranged in a completely randomized design with 10 treatments,
including a negative control, application of only F. oxysporum F.28.1A, application of both
F. oxysporum F.28.1A and fungicide chemicals, application of both F. oxysporum F.28.1A
and Trichoderma spp. DHCT, application of T. yunnanense T-02B1, application of T. lentiforme
T-18B2, application of T. asperellum T-20B1, application of T. hamatum T-28B1, application
of T. hamatum T-29A1 and a mixture of the five selected isolates of Trichoderma
spp. with their total population equal to that in individual strain application. The results
showed that the five mixed isolates of Trichoderma had a synergistic effect on the reduction
of the disease’s prevalence by 35% compared to the negative control treatment.
FUNDING
This project was funded by the author’s affiliated institution.
RESPONSIBLE EDITOR
Lidia Irzykowska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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