ORIGINAL ARTICLE
Evaluation of citronella oil nanoemulsion formulation against the insect-stored pest Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae)
1
Research Center for Estate Crops, Organization Research for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong, Indonesia
2
Agricultural Production, Politeknik Negeri Jember, Jember, Indonesia
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-02-14
Acceptance date: 2024-04-19
Online publication date: 2024-08-19
Corresponding author
Journal of Plant Protection Research 2024;64(3):288-297
HIGHLIGHTS
- Essential oils (EOs) from citronella, were tested as biopesticides against the storage pest, Callosobruchus maculatus
- Citronella nanoemulsion has higher repellency and toxic effect compared to the conventional form.
- Citronella nanoemulsion has the potential to develop as an insecticide to control Callosobrochus maculatus
KEYWORDS
TOPICS
ABSTRACT
Citronella (Cymbopogon nardus) produces essential oil and has the potential to be
developed as a botanical insecticide. However, in its development, botanical insecticides
encountered several obstacles. Utilizing nanotechnology in nanoemulsion preparations is
one method to overcome these challenges. This research aimed to determine the contents
of the citronella oil nanoemulsion (CiONano) and citronella oil non-nano emulsion
(CiONonNano) formulations and evaluate the toxicity, repellency, and prevention of
oviposition against female adults of Callosobruchus maculatus. This was the first work
to evaluate the nanoemulsion of citronella oil prepared from spontaneous emulsification
against C. maculatus. Chemical content testing used the GCMS method. A toxicity test
using the contact method (LC50), used a probit program, while testing for repellency and
oviposition deterrence was carried out using the no-choice method. The highest chemical
component of CiONonNano and CiONano was citronella (37.56 and 38.97%, respectively),
followed by citronellol (17.71 and 18.99%, consecutively) and geraniol (14.78 and 15.38%,
respectively). In general, the CiONano formulation showed higher repellency and toxicity
than CiONonNano. The LC50 values of CiONano were 10.03%. These values were 4.49 times
lower than the LC50 of CiONonNano. However, the results of the oviposition inhibition test
showed different results, the CiONonNano formulation had a higher oviposition inhibition
capacity for adult female C. maculatus than CiONano. As a result, it is necessary to optimize
the CiONano formulation to obtain consistent results in controlling C. maculatus.
ACKNOWLEDGEMENTS
The authors wish to thank the Indonesian Assessment
Institute of Instrument and Standard for Spices, Medicinal and Aromatic Crops (formerly the Indonesian Spices and Medicinal Crops Research Institute)
for supporting and facilitating us.
RESPONSIBLE EDITOR
Opender Koul
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (35)
1.
Aswathanarayan J.B., Vittal R.R. 2019. Nanoemulsions and their potential applications in food industry. Frontiers Sustainability in Food System 3: 1–21. DOI: https://doi.org/10.3389/fsufs.....
2.
Baliyarsingh B., Chandan K.P. 2023. Prospects of plant-derived metallic nanopesticides against storage pests – a review. Journal of Agriculture and Food Research 14: 100687. DOI: https://doi.org/10.1016/j.jafr....
3.
Boodram R., Khan A. 2019. Bioactivity of Citrus aurantifolia, Citrus limon and Piper nigrum essential oils on Callosobruchus maculatus (F.) (Coleoptera:Bruchidae). Journal of Biopesticide 12: 76–82. [Available on: http://www.jbiopest.com/users/....] [Accessed 25 November 2023].
4.
Brito N.F., Oliveira D.S., Santos T.C., Moreira M.F., Melo A.C.A. 2020. Current and potential biotechnological applications of odorant-binding proteins. Applied of Microbiology and Biotechnology 104: 8631–8648. DOI: https://doi.org/10.1007/s00253....
5.
Caballero-Gallardo K., Rodriguez-Niño D., Fuentes-Lopez K., Stashenko E., Olivero-Verbel J. 2021. Chemical composition and bioactivity of essential oils from Cymbopogon nardus L. and Rosmarinus officinalis L. against Ulomoides dermestoides (Fairmaire, 1893) (Coleoptera: Tenebrionidae). Journal of Essential Oil-Bearing Plants 24: 547–560. DOI: https://doi.org/10.1080/097206....
6.
Daglish G., Nayak M., Arthur F., Athanassiou C. 2018. Insect pest management in stored grain. p. 45–64. In: "Recent Advances in Stored Product Protection" (C.G. Athanassiou, F.H. Arthur, eds.). Springer-Verlag GmbH Berlin Heidelberg, Berlin. DOI: https://doi.org/10.1007/978-3-....
7.
da Silva Moura E., Faroni L.R.D.A., Zanuncio J.C., Heleno F.F., Prates L.H.F. 2019. Insecticidal activity of Vanillosmopsis arborea essential oil and of its major constituent α-bisabolol against Callosobruchus maculatus (Coleoptera: Chrysomelidae). Scientific Reports - Nature 9: 1–8. DOI: https://doi.org/10.1038/s41598....
8.
de Andrade Rodrigues R.M., da Silva Fontes L., de Carvalho Brito R., de Silva Barbosa D.R., das Graças Lopes Citó A.M., do Carmo I.S., de Jesus Sousa E.M., Silva G.N. 2022. A sustainable approach in the management of Callosobruchus maculatus: essential oil of Protium heptaphyllum and its major compound d‑limonene as biopesticides. Journal of Plant Diseases and Protection 129: 831–841. DOI: https://doi.org/10.1007/s41348....
9.
de Souza Alves M., Campos I.M., de Mello Conde de Brito M.C., Cardoso C.M., Pontes E.G., de Souza M.A.A.D. 2019. Efficacy of lemongrass essential oil and citral in controlling Callosobruchus maculatus (Coleoptera: Chrysomelidae), a post-harvest cowpea insect pest. Crop Protection 119: 191–196. DOI: https://doi.org/10.1016/j.crop....
10.
dos Santos V.S.V., da Silva P.H.S., de Souza Loura D. 2022. Biofumigant and repellent activity of citronella essential oil on cowbee beans. Journal of Agricultural Science Research 2: 2–7. DOI: https://doi.org/ 10.22533/at.ed.973262216063.s.
11.
Du Z., Wang C., Tai X., Wang G., Liu, X. 2016. Optimization and characterization of biocompatible oil-in-water nanoemulsion for pesticide delivery. ACS Sustainable Chemistry & Engineering 4: 983–991. DOI: https://doi.org/10.1021/acssus....
12.
Fajarwati D., Toto H., Ludji P.A. 2015. Repellence testing of kaffir lime (Cytrus hystrix) leaf extract against the rice pest Sitophilus oryzae Linnaeus (Coleoptera: Curculionidae) L. Jurnal HPT (Hama Penyakit Tumbuhan) 3 (1): 102–108. [Available on: https://jurnalhpt.ub.ac.id/ind...] [Accessed on 25 November 2023] (in Indonesian).
13.
Hagstrum D.W., Phillips T.W. 2017. Evolution of stored-product entomology: Protecting the world food supply. Annuals Reviews of Entomology 62: 379–397. DOI: https://doi.org/10.1146/annure....
14.
Hassan W., Harahap I.S., Dadang D., Hidayat P. 2018. Efficacy determination of different essential oils against storage pest Callosobruchus maculatus (Coleoptera : Bruchidae). Journal of Entomology and Zoology Studies 6: 1408–1411. [Available on: https://www.entomoljournal.com...] [Accessed on: 20 November 2023].
15.
Hernandez-Lambraño R., Pajaro-Castro N., Caballero-Gallardo K., Stashenko E., Olivero-Verbel J. 2015. Essential oils from plants of the genus Cymbopogon as natural insecticides to control stored product pests. Journal of Stored Product Research 62: 81–83. DOI: https://doi.org/10.1016/j.jspr....
16.
Ismail S.M. 2022. Fumigant residual impacts of Melaleuca alternifolia (Maid. & Betche) Cheel. (Myrtales: Myrtaceae), terpinen-4-ol, and γ-terpinene on Sitophilus oryzae L. (Coleoptera: Curculionidae) on germination of wheat seeds. Journal of Plant Protection Research 62 (3): 258–264. DOI: https://doi.org/10.24425/jppr.....
17.
Jasrotia P., Nagpal M., Mishra C.N., Sharma A.K., Kumar S., Kamble U., Bhardwaj A.K., Kashyap P.L., Kumar S., Singh G.P. 2022. Nanomaterials for postharvest management of insect pests: current state and future perspectives. Frontiers in Nanotechnology 3: 811056. DOI: https://doi.org/10.3389/fnano.....
18.
Jilani G., Su H.C.F. 1983. Laboratory studies on several plant materials as insect repellants for protection of cereal grains. Journal of Economic Entomology 76: 154–157. DOI: https://doi.org/10.1093/jee/76....
19.
Kalpna, Hajam Y. A., Kumar R. 2022. Management of stored grain pest with special reference to Callosobruchus maculatus, a major pest of cowpea: A review. Heliyon: e08703. DOI: https://doi.org/10.1016/j.heli....
20.
Kaur H., Bhardwaj U., Kaur R., Kaur H. 2021. Chemical composition and antifungal potential of citronella (Cymbopogon nardus) leaves essential oil and its major compounds. Journal of Essential Oil Bearing Plants 24: 571–581. DOI: https://doi.org/10.1080/097206....
21.
Kumar S., Nehra M., Dilbaghi N., Marrazza G., Hassan. A., Kim. K. H. 2019. Nano-based smart pesticide formulations: emerging opportunities for agriculture. Journal of Controlled Release 294: 131–153. DOI: https://doi.org/10.1016/j. jconrel.2018.12.012.
22.
Loko Y.L.E., Medegan F.S., Kassa P., Ahouansou C.A., Toffa J., Glinma B., Dougnon V., Koukoui O., Djogbenou S.L., Tamò M. 2021. Bioactivity of essential oils of Cymbopogon citratus (DC) Stapf and Cymbopogon nardus (L.) W. Watson from Benin against Dinoderus porcellus Lesne (Coleoptera: Bostrichidae) infesting yam chips. International Journal of Tropical Insect Science 41: 511–524. DOI: https://doi.org/10.1007/s42690....
23.
Mansouri S.M., Naseri B., Bidar F. 2022. Oviposition preference, population growth and digestive enzymatic function of Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) on six legume grains. Journal of Stored Product Research 99: 10–20. DOI: https://doi.org/10.1016/j.jspr....
24.
McClements D.J. 2012. Nanoemulsions versus microemulsions: terminology, differences, and similarities. Soft Matter Journal 8: 1719–1729. DOI: https://doi.org/10.1039/C2SM06....
25.
Mossa A.-T.H., Afia S.I., Mohafrash S.M.M., Abou-Awad B.A. 2019. Rosemary essential oil nanoemulsion, formulation, characterization and acaricidal activity against the two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Plant Protection Research 59 (1): 102–112. DOI: https://doi.org/10.24425/jppr.....
26.
Nisar M.S., Haq I.U., Ramzan H., Aljedani D.M., Qasim M., Islam W., Khan K.A. 2021. Screening of different legumes for the developmental preference of Callosobruchus maculatus (Bruchidae: Coleoptera). International of Journal Tropical Insect Science 41: 3129–3136. DOI: https://doi.org/10.1007/s42690....
27.
Nwosu L.K., Ikodie G. 2021. Comparative study on the bionomics of Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae) in cowpea varieties (host) and common bean varieties (non‑host): Findings revealed importantfood security information. International Journal of Tropical Insect Science 41: 2679–2694. DOI: https://doi.org/10.1007/s42690....
28.
Padín S.B., Fusé C., Urrutia M.I., dal Bello G.M. 2013. Toxicity and repellency of nine medicinal plants against Tribolium castaneum in stored wheat. Bulletin Insectology 66: 45–49. [Available on: https://core.ac.uk/download/pd...] [Accessed on 20 November 2023].
29.
Rohimatun, Yuliani S., Winasa I.W.W., Dadang. 2020. Efficacy of selected Piperaceae, Asteraceae, and Zingiberaceae plant extracts against Helopeltis antonii Sign. Journal of International Society for Southeast Asian Agricultural Sciences 26 (2): 145–157. [Available on: http://issaasphil.org/wp-conte...] [Accesseed on 29 September 2023].
30.
Rohimatun, Aisyah M.D.N., Puspasari L.T., Rusmin D. 2023. Toxicity and chemical compounds of Piper aduncum fruits extract against storage pest Sitophilus oryzae and Callosobruchus maculatus. IOP Conference Series: Earth and Environmental Sciences 1253: 012001. DOI: https://doi.org/10.1088/1755-1....
31.
Saed B., Ziaee M., Kiasat A.R., Nasab M.J. 2022. Preparation of nanosilica from sugarcane bagasse ash for enhanced insecticidal activity of diatomaceous earth against two stored-products insect pests. Toxin Reviews 41: 516–522. DOI: https://doi.org/10.1080/155695....
32.
Singh R.P., Handa R., Manchanda G. 2021. Nanoparticles in sustainable agriculture: an emerging opportunity. Journal of Controlled Releease 329: 1234–1248. DOI: https://doi.org/ 10.1016/j.jconrel.2020.10.051.
33.
Wakil W., Kavallieratos N.G., Usman M., Gulzar S., El-Shafie H.A.F. 2021. Detection of phosphine resistance in field populations of four key stored-grain insect pests in Pakistan. Insects 12: 1–12. DOI: https://doi.org/10.3390/insect....
34.
Yuliani S., Noveriza R. 2019. Effect of carrier oil and co-solvent on the formation of clove oil nanoemulsion by phase inversion technique. IOP Conference Series: Earth Environtal Science 309: 012036. DOI: https://doi.org/10.1088/1755-1....
35.
Yuliani S., Wahyuningsih K., Hernani H., Herwati H., Hoerudin H., Rahmini R., Noveriza R. 2023. Spontaneous emulsification of citronella oil: effect of processing conditions and production scale. IOP Conference Series.: Earth and Environment Science 1172: 012053. DOI: https://doi.org/10.1088/1755-1....
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
