ORIGINAL ARTICLE
Effects of the nutrition of different diets and lipid content of the insect host larvae, Galleria mellonella on the efficacy of indigenous entomopathogenic nematodes
1
Pests and Plant Protection Department, Agricultural and Biological Research Institute, National Research Center, Cairo, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-03-07
Acceptance date: 2022-05-16
Online publication date: 2022-08-31
Corresponding author
Mona Ahmed Hussein
Pests and Plant Protection Department, Agricultural and Biological Research Institute, National Research Center, Cairo, Egypt
Pests and Plant Protection Department, Agricultural and Biological Research Institute, National Research Center, Cairo, Egypt
Journal of Plant Protection Research 2022;62(3):265-271
HIGHLIGHTS
- Impact of four artificial diets and one natural food on producing larvae suitable for pathogenicity by IJs and suitable for nematode production
- Food supplied in D 2 & 3 was efficient to produce a greater number of larvae as well as the natural food
- Beeswax diet was a good production of Galleria and an alternative suitable food
- Natural beeswax produced the highest numbers of nematodes followed by D1; D3
KEYWORDS
dietsGalleria mellonellaHeterorhabditis bacteriophoralipidsmultiplicationpathogenicitySteinernema carpocapsae
TOPICS
ABSTRACT
Entomopathogenic nematodes (EPNs) are promising as biocontrol agents for the most economically
important insect pest attacking a wide range of host plants. Therefore, the aim of
this work was to study the impact of four artificial diets and one natural food on numbers,
weights, and total lipid content of the greater wax moth larvae, Galleria mellonella (Linnaeus)
(Lepidoptera: Pyralidae) as well as the impact of these diets on the ability of nematode
species Heterorhabditis bacteriophora and Steinernema carpocapsae to infect insects
and multiply inside an insect host which had been reared on one of five different diets (D1,
D2, D3, D4 and D5). The correlation between larvae weight and total lipid content, pathogenicity
or multiplication of nematodes was also studied. The obtained results indicated
that D2, D5 and D3 gave the highest growth or weights of larvae. The larvae produced
weighed 3.71, 3.67 and 3.25 g from 50 g media, respectively. Statistically, larvae weights had
a positive and significant correlation with the lipid content in larvae where r = 0.732. On
the other hand, infective juveniles (IJs) of nematodes produced from insect hosts reared on
D2 and D5 revealed more pathogenicity on larvae, since they caused the highest percent
of mortality, 53.33 and 50.0% for H. bacteriophora, and 56.67 and 53.33% for S. carpocapsae,
respectively. The total lipid content had a positive and highly significant correlation
with the pathogenicity of the two nematode species where r = 0.97 and 0.971, respectively.
Ultimately, the supplied foods of the artificial diets D2, D3 and natural beeswax (D5) gave
the most suitable chance for developing insect growth and increasing the EPN quality and
enhancing the potential of EPNs as biological control agents against different insect pests.
ACKNOWLEDGEMENTS
We wish to sincerely thank the National Research Centre
for financial support received through project No.
12050137.
FUNDING
project No. 12050137
RESPONSIBLE EDITOR
Anna Filipiak
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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