ORIGINAL ARTICLE
Effects of inoculation with a commercial microbial inoculant Bacillus subtilis C-3102 mixture on rice and barley growth and its possible mechanism in the plant growth stimulatory effect
1
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Japan
2
Process Development Laboratories, Asahi Group Holdings, Japan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-12-25
Acceptance date: 2019-06-04
Online publication date: 2019-06-27
Corresponding author
Koki Toyota
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Naka, 184-8588, Koganei, Japan
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Naka, 184-8588, Koganei, Japan
Journal of Plant Protection Research 2019;59(2):193-205
KEYWORDS
Bacillus subtilis C-3102indole-3-acetic acid (IAA)phosphate solubilizationplant growth promoting rhizobacteria (PGPR)protease
TOPICS
ABSTRACT
The effects of a microbial inoculant (Thervelics®: a mixture of cells of Bacillus subtilis
C-3102 and carrier materials) on rice (Oryza sativa cv. Milkyprincess) and barley (Hordeum
vulgare cv. Sachiho Golden) were evaluated in four pot experiments. In the first and
second experiments, the dry matter production of rice and barley increased significantly
by 10–20% with the inoculation of the mixture at a rate of 107 cfu ⋅ g–1 soil compared with
the non-inoculated control. In the third experiment, the growth promoting effects of the
mixture, the autoclaved mixture and the carrier materials were compared. The dry mater
production of rice grains was the highest in the mixture, and it was significantly higher in
the three treatments than in the control, suggesting that the carrier materials may also have
a plant growth promoting effect and the living cells might have an additional stimulatory
effect. To confirm the efficacy of the living cells in the mixture, only B. subtilis C-3102 cells
were used in the fourth experiment. In addition, to estimate the mechanisms in growth
promotion by B. subtilis C-3102, three B. subtilis strains with similar or different properties
in the production of indole-3-acetic acid (IAA), protease and siderophore and phosphatesolubilizing
ability were used as reference strains. Only B. subtilis C-3102 significantly increased
the dry matter production of rice grains and the soil protease activity was consistently
higher in the soil inoculated with B. subtilis C-3102 throughout the growing period.
These results indicate that the microbial inoculant including live B. subtilis C-3102 may
have growth promoting effects on rice and barley.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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